3 research outputs found
Co-infeccao por virus dengue, sorotipos 1 e 4, em paciente de cidade de porte medio no Brasil
RESUMO A co-infecção por dengue vÃrus pode ocorrer em áreas com circulação endêmica, nas quais diferentes sorotipos vêm circulando durante muitos anos. Neste trabalho relatamos um caso de co-infecção por DENV-1/DENV-4 em soro humano, detectado por testes moleculares. Análises filogenéticas das sequências obtidas indicaram a presença do genótipo V e II de DENV-1 e DENV-4, respectivamente.SUMMARY The natural co-infection with dengue virus can occur in highly endemic areas where different serotypes have been observed for many years. We report one case of DENV-1/DENV-4 co-infection in human serum detected by molecular tests. Phylogenetic analysis of the sequences obtained indicated the presence of genotype V and II for DENV-1 and DENV-4, respectively
CO-INFECTION OF DENGUE VIRUS BY SEROTYPES 1 AND 4 IN PATIENT FROM MEDIUM SIZED CITY FROM BRAZIL
Dengue-4 false negative results by Panbio (R) Dengue Early ELISA assay in Brazil
Background: Dengue is a serious public health problem in numerous countries. the ability to rapidly diagnosis dengue is important for patient triage and management. Detection of dengue viral protein, NS1, represents a new approach to dengue diagnosis.Objective: the present study aims to evaluate if there are false negative results using the NS1 Ag rapid assay (Panbio (R) Dengue Early ELISA) in two different epidemiological situations (epidemic and non-epidemic).Study design: 220 serum samples from patients with clinical symptoms of classical dengue fever were tested by NS1 antigen capture ELISA and Multiplex-Nested-PCR.Results: in samples collected in a non-epidemic period we found a 100% agreement of ELISA and RTPCR in dengue negative samples and 85% agreement of ELISA and RT-PCR in dengue positive samples. But when we tested samples during an epidemic period (large DENV-4 outbreak) we found 15% false negative results (p<0.05) in dengue negative samples.Conclusions: Due to false negative results for DENV-4, the sole use of the Panbio Dengue Early ELISA assay as a screening method for monitoring circulating dengue serotypes must be reevaluated. (C) 2013 Elsevier B.V. All rights reserved.Conselho Nacional de Desenvolvimento CientÃfico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de NÃvel Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fac Med Sao Jose do Rio Preto FAMERP, BR-15090000 Sao Jose Do Rio Preto, SP, BrazilUniv Estadual Paulista Julio de Mesquitta & Filho, Sao Jose Do Rio Preto, SP, BrazilUniversidade Federal de São Paulo UNIFESP, São Paulo, BrazilPrefeitura Sao Jose do Rio Preto, Dept Vigilancia Saude, Sao Jose Do Rio Preto, SP, BrazilUniv Fed Mato Grosso, Sinop, Mato Grosso, BrazilTufts Univ, Cummings Sch Vet Med, Dept Infect Dis & Global Hlth, North Grafton, MA USAUniversidade Federal de São Paulo UNIFESP, São Paulo, BrazilFAPESP: 2012/11733-6FAPESP: 2011/10458-9Web of Scienc