Il reddito d’impresa

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Antineoplastic therapy and urinary enzymes. Preliminary note on the determination of urinary N-acetyl-beta-D-glucosaminidase in patients treated with cisplatin

We discussed the diagnostic value of urinary enzymes as non invasive test of renal integrity in medicine. Then we assayed the N-Acetyl-beta-D-glucosaminidase (NAG) excretion in urines of inpatients receiving cis-platinum, a useful anti-cancer but nephrotoxic agent fort the proximal tubule; we used the colorimetric instead of fluorimetric procedure, recently described in detail by Price who tested it on patients with a variety of renal diseases. The results demonstrated the sensibility and the specificity of colorimetric procedure, and therefore we concluded that the assay of urinary NAG activity may be used in the clinical chemistry and toxicology laboratory, because the test is simple to perform

Cadmium distribution within corn plants as a function of cadmium loading of the soil.

Corn is a widespread crop in some industrialized areas of Northern Italy and Europe where it serves for both animal and human nutrition. In the present paper we examined the distribution of Cd within corn plants grown under both hydroponic and field conditions. The results obtained show that Cd accumulation by different plant tissues may reach saturation without sign of phytoxicity. Thus cadmium pollution can be undetected even when corn plants are grown in the presence of a consistent amount of Cd. Its accumulation within plant tissues is responsible for the entry of the metal into the food chain. Implication for human and animal nutrition are discussed

Adipose derived stem cell therapy for intervertebral disc regeneration : an in vitro tissue in alginate capsules

The degenerative pathologies of the intervertebral disc have a remarkable social impact in the industrialized countries and can provide serious disabilities in the population. The current treatment consists of conservative treatments (such as symptomatic pharmacological therapies and physiokinetic therapy) and surgical treatments (intervertebral fusion, total disc replacement, nucleus pulposus (NP) replacement, or surgical exeresis). Recent advances in cell therapy foresee the possibility of regenerating the damaged disc; the autologous disc tissue can be withdrawn, in vitro regenerated, and re-implanted. The aim of this work was to verify whether autologous adipose-derived adult stem cells can improve the quality of an in vitro reconstructed nucleus pulposus tissue. A three-dimensional (3D) co-culture of NP cells and adipose tissue non-adipocyte fraction cells (nAFs) was assessed in a previously developed alginate 3D culture system following the good manufacturing practice guidelines to ensure patient safety for clinical studies. Morphological investigation of cultured and co-cultured cells was performed using transmission electron microscopy and immunofluorescence for collagen type I, aggrecan, CD90, CD34, and vimentin. Results indicate that co-culture of NP and nAFs improves the quality of the in vitro reconstructed tissue in term of extracellular matrix production and 3D cell organization. Technological resources are available for NP cell encapsulation intended for regenerating the intervertebral dis

Adipose Derived Stem Cell Therapy for Intervertebral Disc Regeneration: an in Vitro Reconstructed Tissue in Alginate Capsules

The degenerative pathologies of the intervertebral disc have a remarkable social impact in the industrialized countries and can provide serious disabilities in the population. The current treatment consists of conservative treatments (such as symptomatic pharmacological therapies and physiokinetic therapy) and surgical treatments (intervertebral fusion, total disc replacement, nucleus pulposus (NP) replacement, or surgical exeresis). Recent advances in cell therapy foresee the possibility of regenerating the damaged disc; the autologous disc tissue can be withdrawn, in vitro regenerated, and re-implanted. The aim of this work was to verify whether autologous adipose-derived adult stem cells can improve the quality of an in vitro reconstructed nucleus pulposus tissue. A three-dimensional (3D) co-culture of NP cells and adipose tissue non-adipocyte fraction cells (nAFs) was assessed in a previously developed alginate 3D culture system following the good manufacturing practice guidelines to ensure patient safety for clinical studies. Morphological investigation of cultured and co-cultured cells was performed using transmission electron microscopy and immunofluorescence for collagen type I, aggrecan, CD90, CD34, and vimentin. Results indicate that co-culture of NP and nAFs improves the quality of the in vitro reconstructed tissue in term of extracellular matrix production and 3D cell organization. Technological resources are available for NP cell encapsulation intended for regenerating the intervertebral disc

Canine circovirus in foxes from northern Italy: Where did it all begin?

Canine circovirus (CanineCV) is a recently identified virus affecting both domestic and wild carnivores, including foxes, sometimes in presence of severe clinical signs. Its circulation in wild animals can thus represent a potential threat for endangered species conservation and an infection source for dogs. Nevertheless, no data were available on its circulation in the Alps region of Northern Italy. In the present study, samples collected from 186 foxes in the period 2009\u20132020 from Valle d\u2019Aosta and Veneto regions were tested using a real-time PCR assay, demonstrating a viral circulation of approximatively 2\u20135%, depending on the considered regions. Two complete or almost complete genome sequences were obtained, highlighting that the detected strains were part of a so defined \u201cfox only\u201d clade, which suggests that, despite common contact opportunities, Alps foxes are not involved in frequent transmission events to domestic dogs. Such genetic isolation could be at least partially attributed to some sort of independent evolution occurred in the foxes, leading to species barrier. Additionally, CanineCV strains in foxes from Italy were unexpectedly related to those previously identified in foxes from the United Kingdom and Scandinavian area. Combining the history of fox distribution in Europe since the last glacial maximum (LGM) with the viral history allowed us to speculate a long-standing coexistence between European canine circovirus and this host, justifying the peculiar geographic distribution and evolutionary paths of the fox infecting clade