1,225 research outputs found
Dihydropteroate synthase gene mutations in Pneumocystis and sulfa resistance
Pneumocystis pneumonia (PCP) remains a major cause of illness and death in HIV-infected persons. Sulfa drugs, trimethoprim-sulfamethoxazole (TMP-SMX) and dapsone are mainstays of PCP treatment and prophylaxis. While prophylaxis has reduced the incidence of PCP, its use has raised concerns about development of resistant organisms. The inability to culture human Pneumocystis, Pneumocystis jirovecii, in a standardized culture system prevents routine susceptibility testing and detection of drug resistance. In other microorganisms, sulfa drug resistance has resulted from specific point mutations in the dihydropteroate synthase (DHPS) gene. Similar mutations have been observed in P. jirovecii. Studies have consistently demonstrated a significant association between the use of sulfa drugs for PCP prophylaxis and DHPS gene mutations. Whether these mutations confer resistance to TMP-SMX or dapsone plus trimethoprim for PCP treatment remains unclear. We review studies of DHPS mutations in P. jirovecii and summarize the evidence for resistance to sulfamethoxazole and dapsone
Post-Prior discrepancies in CDW-EIS calculations for ion impact ionization fully differential cross sections
In this work we present fully differential cross sections (FDCSs)
calculations using post and prior version of CDW--EIS theory for helium single
ionization by 100 MeV C amu and 3.6 MeV amu Au and
Au ions. We performed our calculations for different momentum transfer
and ejected electron energies. The influence of internuclear potential on the
ejected electron spectra is taken into account in all cases. We compare our
calculations with absolute experimental measurements. It is shown that prior
version calculations give better agreement with experiments in almost all
studied cases.Comment: 9 pages, 7 figure
Adiabatic following criterion, estimation of the nonadiabatic excitation fraction and quantum jumps
An accurate theory describing adiabatic following of the dark, nonabsorbing
state in the three-level system is developed. An analytical solution for the
wave function of the particle experiencing Raman excitation is found as an
expansion in terms of the time varying nonadiabatic perturbation parameter. The
solution can be presented as a sum of adiabatic and nonadiabatic parts. Both
are estimated quantitatively. It is shown that the limiting value to which the
amplitude of the nonadiabatic part tends is equal to the Fourier component of
the nonadiabatic perturbation parameter taken at the Rabi frequency of the
Raman excitation. The time scale of the variation of both parts is found. While
the adiabatic part of the solution varies slowly and follows the change of the
nonadiabatic perturbation parameter, the nonadiabatic part appears almost
instantly, revealing a jumpwise transition between the dark and bright states.
This jump happens when the nonadiabatic perturbation parameter takes its
maximum value.Comment: 33 pages, 8 figures, submitted to PRA on 28 Oct. 200
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