42 research outputs found
A Novel Obligate Intracellular Gamma-Proteobacterium Associated with Ixodid Ticks, Diplorickettsia massiliensis, Gen. Nov., Sp. Nov
Background: Obligate intracellular bacteria of arthropods often exhibit a significant role in either human health or arthropod ecology. Methodology/Principal Findings: An obligate intracellular gamma-proteobacterium was isolated from the actively questing hard tick Ixodes ricinus using mammalian and amphibian cell lines. Transmission electron microscopy revealed a unique morphology of the bacterium, including intravacuolar localization of bacteria grouped predominantly in pairs and internal structures composed of electron-dense crystal-like structures and regular multilayer sheath-like structures. The isolate 20B was characterized to determine its taxonomic position using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that this strain belongs to the family Coxiellaceae, order Legionellales of Gamma-proteobacteria, and the closest relatives are different Rickettsiella spp. The level of 16S rRNA gene sequence similarity between strain 20B and other recognized species of the family was below 94.5%. Partial sequences of the rpoB, parC and ftsY genes confirmed the phylogenetic position of the new isolate. The G+C content estimated on the basis of whole genome analysis of strain 20B was 37.88%. On the basis of its phenotypic and genotypic properties, together with phylogenetic distinctiveness, we propose that strain 20B to be classified in the new genus Diplorickettsia as the type strain of a novel species named Diplorickettsia massiliensis sp. nov. Conclusions/Significance: Considering the source of its isolation (hard tick, often biting humans) the role of this bacterium in the pathology of humans, animals and ticks should be further investigated
The Burma-China Railway: A Case Study of the Commercial and Official Mentality in Nineteenth Century Empire Building
Étude du phénomène de l’induction de facteurs bactériolytiques chez les lépidoptères: Inhibition de la production du lysozyme chez les larves deGalleria mellonella [Lep.: Pyralidae] par l’Actinomycine D et la Cycloheximide
Evolution du taux de gammaglobulines chez les patients atteints de leucémie lymphoïde chronique traités par ibrutinib
# 11-12International audienc
Facettes de la recherche médicale et de la gestion du VIH-sida dans le système de santé chinois : un autre exemple d'adaptation locale de la biomédecine
Essai en culture de choux-fleurs du virus de la polyédrose nucléaire deMamestra brassicae [Lep.: Noctuidae]
Une faible charge allélique JAK2V617F pourrait aider à identifier de nouveaux sous-groupes de polyglobulie de Vaquez et de thrombocytémie essentielle
# 10-12International audienc