Measurement of long-range steric repulsions between microspheres due to an adsorbed polymer

We have measured the interparticle potential between pairs of micron-sized silica spheres induced by adsorbed polyethylene oxide polymer using a line-scanned optical tweezer. We found this long-range steric repulsion to be exponential over the range of energies (0.1kBT–5kBT) and polymer molecular weights (452 000–1 580 000) studied, and that the potential scaled with the polymer’s radius of gyration RG. The potential’s exponential decay length was about 0.6RG and its range was about 4RG, although both parameters varied significantly from one pair of spheres to another. The potential’s exponential prefactor was greater than mean-field predictions

Interaction between Faraday rotation and Cotton-Mouton effects in polarimetry modeling for NSTX

The evolution of electromagnetic wave polarization is modeled for propagation in the major radial direction in the National Spherical Torus Experiment (NSTX) with retroreflection from the center stack of the vacuum vessel. This modeling illustrates that the Cotton-Mouton effect-elliptization due to the magnetic field perpendicular to the propagation direction-is shown to be strongly weighted to the high-field region of the plasma. An interaction between the Faraday rotation and Cotton-Mouton effects is also clearly identified. Elliptization occurs when the wave polarization direction is neither parallel nor perpendicular to the local transverse magnetic field. Since Faraday rotation modifies the polarization direction during propagation, it must also affect the resultant elliptization. The Cotton-Mouton effect also intrinsically results in rotation of the polarization direction, but this effect is less significant in the plasma conditions modeled. The interaction increases at longer wavelength, and complicates interpretation of polarimetry measurements.Comment: Contributed paper published as part of the Proceedings of the 18th Topical Conference on High-Temperature Plasma Diagnostics, Wildwood, New Jersey, May, 201

German morphosyntactic gender and lexical access

Previous eye-tracking research has shown that, during spoken-word recognition, gender marking on preceding articles restricts the competitor set to gender-matching nouns: Upon hearing “Cliquez sur le[masc] bouton” (‘Click on the button’), French listeners did not take the picture of a gender-mismatching ‘bottle’ (bouteille[fem]) into consideration, despite onset similarity between bouton and bouteille (Dahan et al., 2000) In the interpretation of the gender effect, two issues need to be distinguished: When does gender information infl uence noun recognition? Do gender cues pre-activate gendermatching nouns, or does the effect set in when the noun onset is heard? At what level of processing does gender have an effect? What types of representations are involved: shallow co-occurence frequencies or deeper morphosyntactic gender categories? In another experiment, Dahan et al. (2000) found that, by itself, a gender marked article does not seem to prime all gender-matching nouns: Hearing “la[fem] louche” (‘the ladle’) did not increase fixations to a non-onsetoverlapping ‘sock’, chaussette[fem]. This goes against a pre-activation account

The Military : Schottische

https://digitalcommons.library.umaine.edu/mmb-ps/1819/thumbnail.jp

Activation of regulatory T cells triggers specific changes in glycosylation associated with Siglec-1-dependent inflammatory responses [version 1; peer review: 2 approved]

Background: Siglec-1 is a macrophage lectin-like receptor that mediates sialic acid-dependent cellular interactions. Its upregulation on macrophages in autoimmune disease was shown previously to promote inflammation through suppressing the expansion of regulatory T cells (Tregs). Here we investigate the molecular basis for Siglec-1 binding to Tregs using in vitro-induced cells as a model system. Methods: Glycosylation changes that affect Siglec‑1 binding were studied by comparing activated and resting Tregs using RNA-Seq, glycomics, proteomics and binding of selected antibodies and lectins. A proximity labelling and proteomics strategy was used to identify Siglec-1 counter-receptors expressed on activated Tregs. Results: Siglec-1 binding was strongly upregulated on activated Tregs, but lost under resting conditions. Glycomics revealed changes in N-glycans and glycolipids following Treg activation and we observed changes in expression of multiple 'glycogenes' that could lead to the observed increase in Siglec-1 binding. Proximity labelling of intact, living cells identified 49 glycoproteins expressed by activated Tregs that may function as Siglec-1 counter-receptors. These represent ~5% of the total membrane protein pool and were mainly related to T cell activation and proliferation. We demonstrate that several of these counter-receptors were upregulated following activation of Tregs and provide initial evidence that their altered glycosylation may also be important for Siglec-1 binding. Conclusions: We provide the first comprehensive analysis of glycan changes that occur in activated Tregs, leading to recognition by the macrophage lectin, Siglec-1 and suppression of Treg expansion. We furthermore provide insights into glycoprotein counter-receptors for Siglec-1 expressed by activated Tregs that are likely to be important for suppressing Treg expansion

Hydrodynamic Coupling of Two Brownian Spheres to a Planar Surface

We describe direct imaging measurements of the collective and relative diffusion of two colloidal spheres near a flat plate. The bounding surface modifies the spheres' dynamics, even at separations of tens of radii. This behavior is captured by a stokeslet analysis of fluid flow driven by the spheres' and wall's no-slip boundary conditions. In particular, this analysis reveals surprising asymmetry in the normal modes for pair diffusion near a flat surface.Comment: 4 pages, 4 figure

Like-charge attraction through hydrodynamic interaction

We demonstrate that the attractive interaction measured between like-charged colloidal spheres near a wall can be accounted for by a nonequilibrium hydrodynamic effect. We present both analytical results and Brownian dynamics simulations which quantitatively capture the one-wall experiments of Larsen and Grier (Nature 385, p. 230, 1997).Comment: 10 pages, 4 figure

New Pseudo-Phase Structure for α\alpha-Pu

In this paper we propose a new pseudo-phase crystal structure, based on an orthorhombic distortion of the diamond structure, for the ground-state $\alpha$-phase of plutonium. Electronic-structure calculations in the generalized-gradient approximation give approximately the same total energy for the two structures. Interestingly, our new pseudo-phase structure is the same as the Pu $\gamma$-phase structure except with very different b/a and c/a ratios. We show how the contraction relative to the $\gamma$ phase, principally in the $z$ direction, leads to an $\alpha$-like structure in the [0,1,1] plane. This is an important link between two complex structures of plutonium and opens new possibilities for exploring the very rich phase diagram of Pu through theoretical calculations