1,300 research outputs found
Resegmentation is an ancestral feature of the gnathostome vertebral skeleton
Ā© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Criswell, K. E., & Gillis, J. A. Resegmentation is an ancestral feature of the gnathostome vertebral skeleton. Elife, 9, (2020): e51696, doi:10.7554/elife.51696.The vertebral skeleton is a defining feature of vertebrate animals. However, the mode of vertebral segmentation varies considerably between major lineages. In tetrapods, adjacent somite halves recombine to form a single vertebra through the process of āresegmentationā. In teleost fishes, there is considerable mixing between cells of the anterior and posterior somite halves, without clear resegmentation. To determine whether resegmentation is a tetrapod novelty, or an ancestral feature of jawed vertebrates, we tested the relationship between somites and vertebrae in a cartilaginous fish, the skate (Leucoraja erinacea). Using cell lineage tracing, we show that skate trunk vertebrae arise through tetrapod-like resegmentation, with anterior and posterior halves of each vertebra deriving from adjacent somites. We further show that tail vertebrae also arise through resegmentation, though with a duplication of the number of vertebrae per body segment. These findings resolve axial resegmentation as an ancestral feature of the jawed vertebrate body plan.Royal Society (NF160762)
Katharine E Criswell
Royal Society (UF130182)
J. Andrew Gillis
Marine Biological Laboratory
Katharine E. Criswel
Lunar interactions: Abstracts of papers presented at the Conference on Interactions of the Interplanetary Plasma with the Modern and Ancient Moon
Reviewed are the active mechanisms relating the moon to its environment and the linkage between these mechanisms and their records in the lunar sample and geophysical data. Topics: (1) large scale plasma interactions with the moon and non-magnetic planets; (2) ancient and present day lunar surface magnetic and electric fields; (3) dynamics and evolution of the lunar atmosphere; (4) evolution of the solar plasma; (5) lunar record of solar radiations; (6) non-meteoritic and meteoritic disturbance and transport of lunar surface materials; and (7) future lunar exploration
Deconstructing Social Media: An Analysis of Twitter and Facebook Use in the Publishing Industry
The article analyses the social media activity around two genre fiction titles published in the UK. The research is focused on the platforms Twitter and Facebook as they are the sites currently most used as marketing tools by the publishing industry. Over 10,000 social media posts were collected and categorised to create a timeline of social media activity for two case studies. The findings were then compared to sales data from Nielsen BookScan to give an understanding to the value of social media marketing in the publishing industry. The findings show that social media is most effective as a marketing platform when there is already an established community, allowing publishers to converse with readers. While social media is less effective at marketing new books written by debut authors with no existing readership, it is none the less an important tool in the marketing plan as it provides a platform to engage with readers around significant events
Early detection of disease program: Evaluation of the cellular immune response
Surfaces of normal, cultured, and mitogen-stimulated mouse lymphoid cells were examined by scanning electron microscopy (SEM). Lymphocytes with smooth, highly villous and intermediate surfaces were observed in cell suspensions from both spleens and thymuses of normal mice and from spleens of congenitally athymic (nude) mice. Several strain-specific surface features were noted, including the spine-like appearance of microvilli on C57B1/6 lymphocytes. Although thymus cell suspensions contained somewhat more smooth cells than did spleen cell preparations, lymphocyte derivation could not be inferred from SEM examination. Studies of cells stimulated with mitogenic agents for thymus-derived lymphocytes (concanavalin A) or for bone marrow-derived lymphocytes (lipopolysaccharide) suggested that, in the mouse, development of a complex villous surface is a general concomitant of lymphocyte activation and transformation
Cost Comparison of Methyl Bromide and Sulfuryl Fluoride (ProFumeĀ®) for Fumigating Food Processing Facilities, Warehouses, and Cocoa Beans
Costs of fumigating a food processing/warehouse facility using methyl bromide and sulfuryl fluoride (ProFumeĀ®) were compared using an economic-engineering approach. The two fumigants were also compared for fumigating cocoa beans. Sulfuryl fluoride is economically feasible for cocoa beans. It is economically feasible for some, but not all, applications in food processing facilities and warehouses. Keywords: Economics, Cost, Benefit, Fumigation methyl bromide, Sulfuryl fluoride, ProFume
Material variability and wood joist floor response
CER75-76RVG-MEC-JRG-MDV-JB46.May 1976.Includes bibliographical references
Conserved and unique transcriptional features of pharyngeal arches in the skate (Leucoraja erinacea) and evolution of the jaw
Ā© The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Hirschberger, C., Sleight, V. A., Criswell, K. E., Clark, S. J., & Gillis, J. A. Conserved and unique transcriptional features of pharyngeal arches in the skate (Leucoraja erinacea) and evolution of the jaw. Molecular Biology and Evolution, (2021): msab123, https://doi.org/10.1093/molbev/msab123The origin of the jaw is a long-standing problem in vertebrate evolutionary biology. Classical hypotheses of serial homology propose that the upper and lower jaw evolved through modifications of dorsal and ventral gill arch skeletal elements, respectively. If the jaw and gill arches are derived members of a primitive branchial series, we predict that they would share common developmental patterning mechanisms. Using candidate and RNAseq/differential gene expression analyses, we find broad conservation of dorsoventral patterning mechanisms within the developing mandibular, hyoid and gill arches of a cartilaginous fish, the skate (Leucoraja erinacea). Shared features include expression of genes encoding members of the ventralising BMP and endothelin signalling pathways and their effectors, the joint markers nkx3.2 and gdf5 and pro-chondrogenic transcription factor barx1, and the dorsal territory marker pou3f3. Additionally, we find that mesenchymal expression of eya1/six1 is an ancestral feature of the mandibular arch of jawed vertebrates, while differences in notch signalling distinguish the mandibular and gill arches in skate. Comparative transcriptomic analyses of mandibular and gill arch tissues reveal additional genes differentially expressed along the dorsoventral axis of the pharyngeal arches, including scamp5 as a novel marker of the dorsal mandibular arch, as well as distinct transcriptional features of mandibular and gill arch muscle progenitors and developing gill buds. Taken together, our findings reveal conserved patterning mechanisms in the pharyngeal arches of jawed vertebrates, consistent with serial homology of their skeletal derivatives, as well as unique transcriptional features that may underpin distinct jaw and gill arch morphologies.This work was supported by a Biotechnology and Biological Sciences Research Council Doctoral Training Partnership studentship to CH, by a Wolfson College Junior Research Fellowship and MBL Whitman Early Career Fellowship to VAS, and by a Royal Society
University Research Fellowship (UF130182 and URF\R\191007), Royal Society Research Grant (RG140377) and University of Cambridge Sir Isaac Newton Trust Grant (14.23z) to JAG
Layered beam systems with interlayer slip
CER72-73MFK-JRG-MDV-MEC-JB27.December, 1972.Includes bibliographical references (pages 118-120).This report describes a portion of a study on wood joist floor systems. The objective of this portion of the study is to develop a general theory for the analysis of the layered beams within the system including the effects of interlayer slip. The development leads to the governing equations for beams having a single axis of symmetry and an arbitrary number of layers and fastened together with mechanical connectors. Solutions to the governing equations are presented in closed form and in finite difference approximation. These solutions show the effect of interlayer connection on the deflection. In all cases, consideration is given to variation of properties along the beam length. The results of some tests are presented. The proposed theory is shown to agree favorably with the results obtained in the tests
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