Methylenetetrahydrofolate reductase gene polymorphisms in cardiometabolic diseases

Although there is growing evidence that C677T polymorphism of the methylenetetrahydrofolate reductase (MTHFR) gene could be considered a risk factor for cardiometabolic diseases associated with elevated levels of homocysteine, the clinical impact and efficiency of therapy remain a matter of debate. The role of A1298C polymorphism of MTHFR in these diseases is still not clearly defined. Most of the studies have shown the correlations between homozygosity for the T677 allele of the MTHFR gene and homocysteine-related cardiometabolic diseases including the metabolic syndrome, diabetes mellitus, ischemic cardiopathy, stroke, and venous thromboembolism. The proposed pathological mechanism of hyperhomocysteinemia involves prothrombotic effects and endothelial dysfunction. Therapy with vitamins B may decrease the homocysteine level in cases with C677T polymorphism whereas the reducing effect on cardiovascular events is not significant.Biomedical Reviews 2008; 19: 49-52

Wood Species Identification, A Challenge of Scientific Conservation

Wood species identification is an important step in the scientific approach of conservation of the wooden cultural heritage. The paper refers to the microscopic identification of the wooden species for two artisanal objects, investigated for conservation purposes. A previous macroscopic analysis of these objects, after thorough cleaning of the surfaces offered some basic information on the possible wood species involved, but due to the degradation of the support this was not conclusive for some elements of these objects, so that relevant samples were taken out, prepared and investigated. The identified wooden species were: poplar (Populus spp), sycamore (Acer pseudoplatanus), fir and beech (Fagus sylvatica). This identification was based on the microscopic keys of wood identification, reference microscopic slides of the respective wood species and microscopic measurements followed by data processing employing the ImageJ software

A collaborative evaluation of LC-MS/MS based methods for BMAA analysis: soluble bound BMAA found to be an important fraction.

Exposure to β-Ν-methylamino-l-alanine (BMAA) might be linked to the incidence of amyotrophic lateral sclerosis, Alzheimer's disease and Parkinson's disease. Analytical chemistry plays a crucial role in determining human BMAA exposure and the associated health risk, but the performance of various analytical methods currently employed is rarely compared. A CYANOCOST initiated workshop was organized aimed at training scientists in BMAA analysis, creating mutual understanding and paving the way towards interlaboratory comparison exercises. During this workshop, we tested different methods (extraction followed by derivatization and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) analysis, or directly followed by LC-MS/MS analysis) for trueness and intermediate precision. We adapted three workup methods for the underivatized analysis of animal, brain and cyanobacterial samples. Based on recovery of the internal standard D3BMAA, the underivatized methods were accurate (mean recovery 80%) and precise (mean relative standard deviation 10%), except for the cyanobacterium Leptolyngbya. However, total BMAA concentrations in the positive controls (cycad seeds) showed higher variation (relative standard deviation 21%-32%), implying that D3BMAA was not a good indicator for the release of BMAA from bound forms. Significant losses occurred during workup for the derivatized method, resulting in low recovery ( < 10%). Most BMAA was found in a trichloroacetic acid soluble, bound form and we recommend including this fraction during analysis

Experimental Analyses for The Mechanical Behavior of Pressed All-Ceramic Molar Crowns with Anatomical Design

Ceramic restorations show considerable variation in strength and structural reliability regarding to the type of material, and design characteristics. The fracture of ceramics occurs with little or no plastic deformation, with cracks propagated in an unstable manner under applied tensile stresses. The aim of the study was to assess experimental analyses of pressed monolithic ceramic crowns with anatomical design used in the posterior areas in order to understand their mechanical behavior before following their clinical use. Experiments were conducted on a complete molar crown preparation. Experiments show different modes of fracture for the tested samples. Digital images from the fractured pieces of the crowns were used to verify the fragments in all cases final fracture occurred by splitting into two and often more parts. The graphically representation of the displacement depending on the load highlights a series of peaks that can be correlated with cracks occurred in crowns. The development of well-designed mechanical experiments could be useful to help to predict clinical survival of these new all-ceramic restorative techniques and materials. Because failure is often accompanied by complete cracking of the crowns, preliminary research should represents a compulsory goal

Experimental Analyses for The Mechanical Behavior of Pressed All-Ceramic Molar Crowns with Anatomical Design

Ceramic restorations show considerable variation in strength and structural reliability regarding to the type of material, and design characteristics. The fracture of ceramics occurs with little or no plastic deformation, with cracks propagated in an unstable manner under applied tensile stresses. The aim of the study was to assess experimental analyses of pressed monolithic ceramic crowns with anatomical design used in the posterior areas in order to understand their mechanical behavior before following their clinical use. Experiments were conducted on a complete molar crown preparation. Experiments show different modes of fracture for the tested samples. Digital images from the fractured pieces of the crowns were used to verify the fragments in all cases final fracture occurred by splitting into two and often more parts. The graphically representation of the displacement depending on the load highlights a series of peaks that can be correlated with cracks occurred in crowns. The development of well-designed mechanical experiments could be useful to help to predict clinical survival of these new all-ceramic restorative techniques and materials. Because failure is often accompanied by complete cracking of the crowns, preliminary research should represents a compulsory goal

Micro-CT Marginal and Internal Fit Evaluation of CAD/CAM High-Performance Polymer Onlay Restorations

(1) Background: The use of high-performance polymers for fixed restorations requires additional studies regarding their adaptability and processing with CAD/CAM technology. This in vitro study aims to assess the marginal and internal fit of PEEK and PEKK materials using microcomputed tomography. (2) Methods: Twenty-four (n = 8) MOD onlays made of PEKK (Pekkton ivory), unmodified PEEK (Juvora medical), and modified PEEK (BioHPP) were investigated. A typodont mandibular left first molar was scanned to achieve 24 resin, 3D printed abutment teeth. The onlays were fabricated with a five-axis milling machine, and after cementation of the specimens, the marginal (MG) and internal gaps (IG) were evaluated at twelve points in the mesio-distal section and thirteen points in the bucco-lingual section using microcomputed tomography. For statistical data analysis, Wilcoxon signed-rank/paired Student t-Test, Mann–Whitney/unpaired Student t-Test, and one-way ANOVA test were applied. (3) Results: Significant differences (p < 0.05; α = 0.05) were reported between the MG and IG for each material for all three polymers and also among two materials in terms of the MG and IG (except Juvora-BioHPP). The highest IG values were recorded in angular areas (axio-gingival line angle) in the mesio-distal section for all the polymers. (4) Conclusions: For all the materials, MG < IG. The type of polymer influenced the adaptability; the lowest marginal and internal gap mean values were recorded for BioHPP. The analyzed polymer used for onlays are clinically acceptable in terms of adaptability

Adaptability Evaluation of Metal-Ceramic Crowns Obtained by Additive and Subtractive Technologies

(1) Background: Traditional metal-ceramic restorations are considered as a standard in the evaluation of new technologies. A critical factor in their longevity is represented by their adaptability; The purpose of this study was to evaluate the marginal and internal gap of ceramic-fused to metal crowns with frameworks obtained by additive manufacturing (AM) technologies and the influence of veneering process on their fit; (2) Methods: Metal-ceramic crowns have been produced by conventional lost-wax technology (T), digital milling (F), selective laser sintering (SLS) and selective laser melting (SLM). The adaptability was assessed using silicone replicas before and after ceramic veneering; (3) Results: The best values were obtained for the milled group followed closely by SLM and SLS, and a significantly higher gap for casted copings. The veneering process did not significantly influence the adaptability of the crowns, regardless of the manufacturing process used for frameworks. The present study promotes additive technologies (AT) as a fast, efficient, and cost-effective alternative to traditional technology. There are fewer steps in which errors can occur when digital technologies are used and the risk of distortion is diminished. (4) Conclusions: CAD/CAM technologies, both additive and subtractive, represent an excellent option to produce time-effective, precise metal-ceramic crowns with excellent adaptation

Selected Physical and Mechanical Properties of Subfossil Oak (<i>Quercus</i> spp.) Compared to Aged Oak and Recent Oak

Subfossil oak (SO) wood material, originating from three different buried trunks discovered in recent years by excavations in riverbanks on Romanian territory, was analysed in this research. Aged oak recovered from constructions (AO_C) and recent/new oak wood material (NO) were also investigated to provide comparative data for the SO. The oven-dry density and the basic density, the total volumetric and linear swelling and shrinkage coefficients and the compression strength parallel to the grain were the selected physical and mechanical properties considered. The experimental results showed a lower density of SO compared to NO and AO_C tested by up to about 19–20%, alongside a trend of increased dimensional instability, with variability among the tested assortments. The compression strength parallel to the grain was reduced by 19–31% compared to NO. The properties of AO-C were closer to those of NO, but differences between wood materials from different sources and of different ages were registered. A positive linear correlation was found between compression strength parallel to grain and the basic density for all types of material and assortments tested. These comparative results have to be considered by designers and engineers in the valorisation of SO in furniture design and other applications

ARGUMENTS FOR REUSING OLD OAK WOOD RECOVERED FROM DEMOLITION

The main objective of this study was to find scientific arguments in favour of reusing recovered oak wood in order to reintegrate it in new types of structures. For this study six beams were selected, which had been recovered from a house built in 1831. Visual analysis revealed several defects and signs of degradation in the sapwood and inside the cracks. The recovered beams hadn’t been treated with any toxic chemicals. Bending strength, modulus of elasticity and compression strength parallel to the grain were investigated and microscopy and FTIR-ATR spectroscopy were used to check the quality of the apparently sound beam areas also used for the determination of mechanical properties. Macroscopic and microscopic analysis may not reveal any incipient fungal attack but FTIR analysis was proven to be effective. The obtained results open a new perspective for the future use of recovered old oak wood, due to the adequate mechanical properties and to the assumed means of eliminating degraded areas

Surface Characteristics of High Translucent Multilayered Dental Zirconia Related to Aging

(1) Background: The purpose of this study was to evaluate the differences in terms of surface characteristics (roughness, topography, microhardness) among layers for multi-layered high translucent and super-high translucent zirconia and the influence of finishing and aging on surface characteristics and microstructure. (2) Methods: Three types of translucent multilayer zirconia were evaluated: STML (4Y-TZP); IPS e.maxZirCAD CEREC/in Lab MT Multi (4Y-TZP + 5Y-TZP); CeramillZolidfx ML (5Y-TZP). Ninety-six plate-shaped samples (32 for a material), 16 mm × 14 mm × 1 mm size, were cut with a precision disc, polished on both sides with sand papers and sintered respecting the manufacturer’s protocol. Half of the specimens (16) were finished by polishing and the other half by glazing and then equally divided into one control group and one group subject to aging by autoclaving (1 h, 134 °C, 0.2 MPa), resulting in four groups of eight samples, for each zirconia. The specimens were evaluated in three areas: cervical, medium, incisal-of each glazed or polished surface, before and after aging. Tests were performed to determine the surface roughness using a profilometer; the surface topography by an atomic force microscope (AFM) and a scanning electron microscope (SEM). Microhardness was recorded using a microhardness tester. Statistical analyses were performed using two-way ANOVA test, unpaired sample t-Test, paired sample t-Test (α = 0.05) and Pearson’s correlation. (3) Results: Before and after autoclaving, for glazed samples significance (p p > 0.05) were reported between the three areas, on glazed or polished surfaces of a material. Although, after aging an increase in surface roughness was observed both on glazed and polished samples, statistical differences were found for STML (p 0.05). No significant differences (p> 0.05) concerning microhardness among the same areas, on glazed and polished surfaces, recorded before and after aging, except CeramillZolidfx ML glazed samples. (4) Conclusions: For tested zirconia materials no significant differences among layers were registered regarding surface characteristics. Surface treatment (glazing or polishing) has a significant impact on surface roughness and microhardness. Both before and after aging, the surface roughness values for the glazed samples were higher than for those polished. The super translucent 4Y-TZP material was more affected by aging compared to the super-high translucent 5Y-TZP material. The combined material revealed similarities for each layer corresponding to the microstructure