Co-existing with the alien: evidence for environmental control on trophic interactions between a native (Atherina boyeri) and a non-indigenous fish species (Gambusia holbrooki) in a Mediterranean coastal ecosystem

Biological invasions are a widespread problem worldwide, as invasive non-indigenous species (NIS) may affect native populations through direct (e. g., predation) or indirect (e.g., competition) trophic interactions, leading to changes in the food web structure. The trophic relationships of the invasive eastern mosquitofish Gambusia holbrooki and the native big-scale sand smelt Atherina boyeri coexisting in three Mediterranean coastal ponds characterized by different trophic statuses (from oligotrophic to hypereutrophic) were assessed in spring through isotopic niche analysis and Bayesian mixing models. The two fish relied on the distinctive trophic pathways in the different ponds, with the evidence of minimal interspecific niche overlap indicating site-specific niche divergence mechanisms. In more detail, under hypereutrophic and mesotrophic conditions, the two species occupied different trophic positions but relying on a single trophic pathway, whereas, under oligotrophic conditions, both occupied a similar trophic position but belonging to distinct trophic pathways. Furthermore, the invaders showed the widest niche breadth while the native species showed a niche compression and displacement in the ponds at a higher trophic status compared to the oligotrophic pond. We argue that this may be the result of an asymmetric competition arising between the two species because of the higher competitive ability of G. holbrooki and may have been further shaped by the trophic status of the ponds, through a conjoint effect of prey availability and habitat complexity. While the high trophic plasticity and adaptability of both species to different environmental features and resource availability may have favored their coexistence through site-specific mechanisms of niche segregation, we provide also empirical evidence of the importance of environmental control in invaded food webs, calling for greater attention to this aspect in future studies

Resistance of seagrass habitats to ocean acidification via altered interactions in a tri-trophic chain

Despite the wide knowledge about prevalent effects of ocean acidification on single species, the consequences on species interactions that may promote or prevent habitat shifts are still poorly understood. Using natural CO2 vents, we investigated changes in a key tri-trophic chain embedded within all its natural complexity in seagrass systems. We found that seagrass habitats remain stable at vents despite the changes in their tri-trophic components. Under high pCO2, the feeding of a key herbivore (sea urchin) on a less palatable seagrass and its associated epiphytes decreased, whereas the feeding on higher-palatable green algae increased. We also observed a doubled density of a predatory wrasse under acidified conditions. Bottom-up CO2 effects interact with top-down control by predators to maintain the abundance of sea urchin populations under ambient and acidified conditions. The weakened urchin herbivory on a seagrass that was subjected to an intense fish herbivory at vents compensates the overall herbivory pressure on the habitat-forming seagrass. Overall plasticity of the studied system components may contribute to prevent habitat loss and to stabilize the system under acidified conditions. Thus, preserving the network of species interactions in seagrass ecosystems may help to minimize the impacts of ocean acidification in near-future oceans.The research was funded by an ASSEMBLE access project within the EU FP7/2007–2013 program (grant agreement n° 227799) hosted by MCG at the SZN. The Portuguese FCT– Fundação para a Ciência e a Tecnologia funded BMC in the ambit of the contract program DL57/2016/CP1361/CT0004 and CCMAR through the project UID/Multi/04326/2019.info:eu-repo/semantics/publishedVersio

Ontogenetic trophic segregation between two threatened smooth-hound sharks in the Central Mediterranean Sea

Elasmobranchs are among the species most threatened by overfishing and a large body of evidence reports their decline around the world. As they are large predators occupying the highest levels of marine food webs, their removal can alter the trophic web dynamic through predatory release effects and trophic cascade. Suitable management of threatened shark species requires a good understanding of their behaviour and feeding ecology. In this study we provide one of the first assessments of the trophic ecology of the \u201cvulnerable\u201d smooth-hounds Mustelus mustelus and M. punctulatus in the Central Mediterranean Sea, based on stomach contents and stable isotope analyses. Ontogenetic diet changes were addressed by comparing the feeding habits of three groups of individuals: juveniles, maturing and adults. Our results highlighted that the two species share a similar diet based mostly on the consumption of benthic crustaceans (e.g. hermit crabs). Their trophic level increases during ontogeny, with adults increasing their consumption of large-sized crustaceans (e.g. Calappa granulata, Palinurus elephas), cephalopods (e.g. Octopus vulgaris) and fish (e.g. Trachurus trachurus). Our results provide also evidence of ontogenetic shifts in diet for both species showing a progressive reduction of interspecific trophic overlap during growth. The results of this study contribute to improve the current knowledge on the trophic ecology of these two threatened sharks in the Strait of Sicily, thus providing a better understanding of their role in the food web

Decline of cardiomyocyte contractile performance and bioenergetic function in socially stressed male rats

Chronic social stress has been epidemiologically linked to increased risk for cardiovascular disease, yet the underlying pathophysiological mechanisms are still largely elusive. Mitochondrial (dys)function represents a potential intersection point between social stress exposure and (mal)adaptive cardiac responses. In this study, we used a rodent model of social stress to study the extent to which alterations in the cellular mechanical properties of the heart were associated with changes in indexes of mitochondrial function. Male adult rats were exposed to repeated episodes of social defeat stress or left undisturbed (controls). ECG signals were recorded during and after social defeat stress. Twenty-four hours after the last social defeat, cardiomyocytes were isolated for analyses of mechanical properties and intracellular Ca(2+) dynamics, mitochondrial respiration, and ATP content. Results indicated that social defeat stress induced potent cardiac sympathetic activation that lasted well beyond stress exposure. Moreover, cardiomyocytes of stressed rats showed poor contractile performance (e.g., slower contraction and relaxation rates) and intracellular Ca(2+) derangement (e.g., slower Ca(2+) clearing), which were associated with indexes of reduced reserve respiratory capacity and decreased ATP production. In conclusion, this study suggests that repeated social stress provokes impaired cardiomyocyte contractile performance and signs of altered mitochondrial bioenergetics in the rat heart. Future studies are needed to clarify the causal link between cardiac and mitochondrial functional remodeling under conditions of chronic social stress

Macroalgae transplant to detect the occurrence of anthropogenic nutrients in seawater of highly tourist beaches in Mediterranean islands

In the Mediterranean region, islands are among the most important tourist destinations, being sites of considerable naturalistic, historical and cultural importance. This is highly beneficial for local economies, but may also represent a threat for the environment, especially during the peak season (i.e. summer). Indeed, the sharp demographic increase concentrated in a relatively short period may negatively affect the quality of coastal marine systems and the provision of ecosystem services, producing in turn negative feedbacks on tourism industry. Nevertheless, the assessment of the environmental impact of tourism on coastal seawater has been seldom addressed. Here, we show the results of the biomonitoring approach adopted in the tourist Island of Rhodes (Greece), in the context of the Interreg Med BLUEISLANDS project. The study involved short-term macroalgae transplantation and incubation, and, through the analysis of nitrogen stable isotopes, provided a time-integrated picture of the occurrence of anthropogenic nutrients, which are indicators of water quality and might be missed by routine water quality monitoring programs. Main findings ruled out a marked input of anthropogenic nutrients potentially threatening the functioning of coastal ecosystems and highlighted overall good environmental conditions. In addition, this approach provided spatial data useful to produce GIS maps, useful tools that may help the decisional process of policy-makers, for adopting management practices to mitigate the environmental impact and foster sustainable touris

Ocean acidification as a driver of community simplification via the collapse of higher-order and rise of lower-order consumers

Increasing oceanic uptake of CO2 is predicted to drive ecological change as both a resource (i.e. CO2 enrichment on primary producers) and stressor (i.e. lower pH on consumers). We use the natural ecological complexity of a CO2 vent (i.e. a seagrass system) to assess the potential validity of conceptual models developed from laboratory and mesocosm research. Our observations suggest that the stressor-effect of CO2 enrichment combined with its resource-effect drives simplified food web structure of lower trophic diversity and shorter length. The transfer of CO2 enrichment from plants to herbivores through consumption (apparent resource-effect) was not compensated by predation, because carnivores failed to contain herbivore outbreaks. Instead, these higher-order consumers collapsed (apparent stressor-effect on carnivores) suggesting limited trophic propagation to predator populations. The dominance of primary producers and their lower-order consumers along with the loss of carnivores reflects the duality of intensifying ocean acidification acting both as resource-effect (i.e. bottom-up control) and stressor-effect (i.e. top-down control) to simplify community and trophic structure and function. This shifting balance between the propagation of resource enrichment and its consumption across trophic levels provides new insights into how the trophic dynamics might stabilize against or propagate future environmental change

Effects of anthropogenic pressures on the seagrass Halophila stipulacea and its associated macrozoobenthic communities in the northern Gulf of Aqaba

Halophila stipulacea is a tropical seagrass species, native to the Red Sea, Persian Gulf, and Indian Ocean, while invasive to the Mediterranean and Caribbean Seas. The benthic fauna assemblages associated with H. stipulacea in its native habitats and the potential effects of anthropogenic stressors on these assemblages remain unknown. We compared meadow characteristics, associated fauna assemblages and trophic niche structures of H. stipulacea from an impacted and a pristine site in the northern Red Sea. Seagrass cover and biomass were higher in the impacted site, however, the associated fauna community was more abundant and diverse in the pristine site. Both meadows showed comparable trophic niches based on stable isotope analysis. This study provides first insights into the macrozoobenthos associated with H. stipulacea in its native habitat and highlights the importance of better understanding the relationship between seagrasses and their associated biota and the potential effects of urbanization on this relationship

Trophic niche influences ingestion of micro- and mesoplastics in pelagic and demersal fish from the Western Mediterranean Sea

Plastic pollution has been extensively documented in the marine food web, but targeted studies focusing on the relationship between microplastic ingestion and fish trophic niches are still limited. In this study we investigated the frequency of occurrence and the abundance of micro- and mesoplastics (MMPs) in eight fish species with different feeding habits from the western Mediterranean Sea. Stable isotope analysis (δ13C and δ15N) was used to describe the trophic niche and its metrics for each species. A total of 139 plastic items were found in 98 out of the 396 fish analysed (25%). The bogue revealed the highest occurrence with 37% of individuals with MMPs in their gastrointestinal tract, followed by the European sardine (35%). We highlighted how some of the assessed trophic niche metrics seem to influence MMPs occurrence. Fish species with a wider isotopic niche and higher trophic diversity were more probable to ingest plastic particles in pelagic, benthopelagic and demersal habitats. Additionally, fish trophic habits, habitat and body condition influenced the abundance of ingested MMPs. A higher number of MMPs per individual was found in zooplanktivorous than in benthivore and piscivorous species. Similarly, our results show a higher plastic particles ingestion per individual in benthopelagic and pelagic species than in demersal species, which also resulted in lower body condition. Altogether, these results suggest that feeding habits and trophic niche descriptors can play a significant role in the ingestion of plastic particles in fish species

Interactions between experience, genotype and sex in the development of individual coping strategies

Coping strategies, the first line of defense against adversities, develop through experience. There is consistent evidence that both genotype and sex contribute to the development of dysfunctional coping, leading to maladaptive outcomes of adverse experiences or to adaptive coping that fosters rapid recovery even from severe stress. However, how these factors interact to influence the development of individual coping strategies is just starting to be investigated. In the following review, we will consider evidence that experience, sex, and genotype influence the brain circuits and neurobiological processes involved in coping with adversities and discuss recent results pointing to the specific effects of the interaction between early experiences, genotype, and stress in the development of functional and dysfunctional coping styles