Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection

Altres ajuts: China Scholarship Council 201608150108Middle East respiratory syndrome coronavirus (MERS-CoV) poses a serious threat to public health. Here, we established an ex vivo alpaca tracheal explant (ATE) model using an air-liquid interface culture system to gain insights into MERS-CoV infection in the camelid lower respiratory tract. ATE can be infected by MERS-CoV, being 103 TCID50/mL the minimum viral dosage required to establish a productive infection. IFNs and antiviral ISGs were not induced in ATE cultures in response to MERS-CoV infection, strongly suggesting that ISGs expression observed in vivo is rather a consequence of the IFN induction occurring in the nasal mucosa of camelids

Evaluation of alpaca tracheal explants as an ex vivo model for the study of Middle East respiratory syndrome coronavirus (MERS-CoV) infection

Middle East respiratory syndrome coronavirus (MERS-CoV) poses a serious threat to public health. Here, we established an ex vivo alpaca tracheal explant (ATE) model using an air-liquid interface culture system to gain insights into MERS-CoV infection in the camelid lower respiratory tract. ATE can be infected by MERS-CoV, being 103 TCID50/mL the minimum viral dosage required to establish a productive infection. IFNs and antiviral ISGs were not induced in ATE cultures in response to MERS-CoV infection, strongly suggesting that ISGs expression observed in vivo is rather a consequence of the IFN induction occurring in the nasal mucosa of camelids.info:eu-repo/semantics/publishedVersio

Desenvolupen un nou model per estudiar la infecció del MERS-coronavirus al laboratori

Investigadors de la Unitat Mixta IRTA-UAB en Salut Animal han desenvolupat i validat un tècnica de cultiu de teixit respiratori de llama que permet estudiar per què els camèlids superen la infecció causada pel MERS-CoV sense desenvolupar signes clínics. Aquesta metodologia permetrà testar productes terapèutics per als humans front a aquest coronavirus, reduint així l'ús d'animals amb finalitats experimentals.Investigadores de la Unidad Mixta IRTA-UAB en Salud Animal han desarrollado y validado un técnica de cultivo de tejido respiratorio de llama que permite estudiar por qué los camélidos superan la infección causada por el MERS-CoV sin desarrollar signos clínicos. La metodología permitirá testar productos terapéuticos para humanos frente a este coronavirus, reduciendo así el uso de animales con fines experimentales.Researchers at the IRTA-UAB Joint Research Unit in Animal Health have developed and validated a technique for the culture of llama respiratory tissue that permits to study why camelids overcome infection caused by MERS-CoV without developing clinical signs. The methodology will allow to test therapeutic products for humans against this coronavirus, thus reducing the use of animals for experimental purposes