A review of research ethics in internet-based research

Internet-based research methods can include; on-line surveys, web page content analysis, videoconferencing for on-line focus groups and/ or interviews, analysis of ‘e’ conversations through social networking sites, email, chat rooms, discussion boards and/ or blogs. Over the last ten years an upsurge in Internet Based Research (IBR) has led to increased interest in IBR and research ethics. Here we present some ethical guidelines for IBR whilst at the same time accepting that it would be unrealistic to expect that any single set of guidelines can cover all ethical situations concerning IBR). There is simply too much diversity across internet cultures, values and modes of operation for that to be the case. Perhaps the most useful solution to the complex challenges of IRB lies with a form of ‘negotiated ethics’, a situated approach grounded in the specifics of the online community, the methodology and the research question(s). This does not mean an ‘anything goes’ relativist approach, rather an open, pluralistic policy in relation to IBR ethical issues (Ess, 2009; AoIR, 2002)

EU migrants as entrepreneurs in Lincolnshire: exploiting the enterprise culture

The arrival of unprecedented numbers of migrant workers in the rural counties of the UK, due to EU enlargement, has created a unique opportunity. Many of these migrants wish to become more than just a source of cheap labour; they aspire to become entrepreneurs. Lincolnshire has already seen a burgeoning of migrant businesses, but these have prospered largely by serving their co-ethnic market. This article examines the barriers and drivers for establishing businesses in the wider regional market and observes the first attempts at providing adequate support mechanisms. With improved language skills, there is potential for migrants to break out of the co-ethnic market and develop sustainable businesses

Rock avalanches and other landslides in the central Southern Alps of New Zealand: a regional study considering possible climate change impacts

Slope instabilities in the central Southern Alps, New Zealand, are assessed in relation to their geological and topographic distribution, with emphasis given to the spatial distribution of the most recent failures relative to zones of possible permafrost degradation and glacial recession. Five hundred nine mostly late-Pleistocene- to Holocene-aged landslides have been identified, affecting 2% of the study area. Rock avalanches were distinguished in the dataset, being the dominant failure type from Alpine slopes about and east of the Main Divide of the Alps, while other landslide types occur more frequently at lower elevations and from schist slopes closer to the Alpine Fault. The pre-1950 landslide record is incomplete, but mapped failures have prevailed from slopes facing west-northwest, suggesting a structural control on slope failure distribution. Twenty rock avalanches and large rockfalls are known to have fallen since 1950, predominating from extremely steep east-southeast facing slopes, mostly from the hanging wall of the Main Divide Fault Zone. Nineteen occurred within 300 vertical metres above or below glacial ice; 13 have source areas within 300 vertical metres of the estimated lower permafrost boundary, where degrading permafrost is expected. The prevalence of recent failures occurring from glacier-proximal slopes and from slopes near the lower permafrost limit is demonstrably higher than from other slopes about the Main Divide. Many recent failures have been smaller than those recorded pre-1950, and the influence of warming may be ephemeral and difficult to demonstrate relative to simultaneous effects of weather, erosion, seismicity, and uplift along an active plate margi

Interview with Ian Cox

Don Dunstan Oral History Project interview transcripts. No conditions are imposed on the reuse of this transcript by the interviewee.Interview with Ian Cox by Bruce Guerin on 5 July 2007. Ian Cox became Director of Aboriginal Affairs and Social Welfare in 1970 and continued to work in this area during the Dunstan era

Identification of Carbohydrate Metabolism Genes in the Metagenome of a Marine Biofilm Community Shown to Be Dominated by Gammaproteobacteria and Bacteroidetes

Polysaccharides are an important source of organic carbon in the marine environment and degradation of the insoluble and globally abundant cellulose is a major component of the marine carbon cycle. Although a number of species of cultured bacteria are known to degrade crystalline cellulose, little is known of the polysaccharide hydrolases expressed by cellulose-degrading microbial communities, particularly in the marine environment. Next generation 454 Pyrosequencing was applied to analyze the microbial community that colonizes and degrades insoluble polysaccharides in situ in the Irish Sea. The bioinformatics tool MG-RAST was used to examine the randomly sampled data for taxonomic markers and functional genes, and showed that the community was dominated by members of the Gammaproteobacteria and Bacteroidetes. Furthermore, the identification of 211 gene sequences matched to a custom-made database comprising the members of nine glycoside hydrolase families revealed an extensive repertoire of functional genes predicted to be involved in cellulose utilization. This demonstrates that the use of an in situ cellulose baiting method yielded a marine microbial metagenome considerably enriched in functional genes involved in polysaccharide degradation. The research reported here is the first designed to specifically address the bacterial communities that colonize and degrade cellulose in the marine environment and to evaluate the glycoside hydrolase (cellulase and chitinase) gene repertoire of that community, in the absence of the biases associated with PCR-based molecular techniques

Human platelet activation by Escherichia coli: roles for FcγRIIA and integrin αIIbβ3

Gram-negative Escherichia coli cause diseases such as sepsis and hemolytic uremic syndrome in which thrombotic disorders can be found. Direct platelet–bacterium interactions might contribute to some of these conditions; however, mechanisms of human platelet activation by E. coli leading to thrombus formation are poorly understood. While the IgG receptor FcγRIIA has a key role in platelet response to various Gram-positive species, its role in activation to Gram-negative bacteria is poorly defined. This study aimed to investigate the molecular mechanisms of human platelet activation by E. coli, including the potential role of FcγRIIA. Using light-transmission aggregometry, measurements of ATP release and tyrosine-phosphorylation, we investigated the ability of two E. coli clinical isolates to activate platelets in plasma, in the presence or absence of specific receptors and signaling inhibitors. Aggregation assays with washed platelets supplemented with IgGs were performed to evaluate the requirement of this plasma component in activation. We found a critical role for the immune receptor FcγRIIA, αIIbβ3, and Src and Syk tyrosine kinases in platelet activation in response to E. coli. IgG and αIIbβ3 engagement was required for FcγRIIA activation. Moreover, feedback mediators adenosine 5’-diphosphate (ADP) and thromboxane A₂ (TxA₂) were essential for platelet aggregation. These findings suggest that human platelet responses to E. coli isolates are similar to those induced by Gram-positive organisms. Our observations support the existence of a central FcγRIIA-mediated pathway by which human platelets respond to both Gram-negative and Gram-positive bacteria