Effects of Andiroba oil (Carapa guianensis) on wound healing in alloxan-diabetic rats

Purpose: To evaluate wound healing in diabetic rats by using topic Andiroba oil (Carapa guianensis). Methods: Six male, adult, Wistar rats were distributed into three groups: Sham group (wound treatment with distilled water); Collagenase group (treatment with collagenase ointment); and Andiroba group (wound treatment with Andiroba oil). The wound was evaluated considering the macroscopic and microscopic parameters. Results: The results indicated differences in the healing of incisional wounds between treatments when compared to control group. Accelerated wound healing was observed in the group treated with Andiroba oil and Collagenase in comparison to control group, especially after the 14th day. Morphometric data confirmed the structural findings. Conclusion: There was significant effect in topical application of Andiroba oil on wound healing in rats with induced diabetes.   Keywords: Medicinal plants. Diabetes Mellitus. Wound healing. Rats

Matrix Microparticles Of Copaiba Oil (Copaifera Langsdorffii) On Renal Physiology: Patent Review

PURPOSE: to demonstrate the effect of matrix microparticles of Copaifera langsdorffii (aerial parts of copaiba), aiming to evaluate the discussions and results of the works that demonstrate Its function on the kidneys as well as its effectiveness. METHODS: A bibliographic study was carried out by consulting public online databases of patents, such as the United States Patent and Trademark office, World Intellectual Property Organization and the National Intellectual Property Institute; The following keywords were used: copaiba oil, renal function, kidney, kidney stones, matrix microparticles Copaifera langsdorffii and lithiasis. In this study were included patents of invention, innovations and utility models in the period from January 2007 to May 2016. RESULTS: One patent register was included regarding a method for obtaining microparticles and compounds, having antilithiasic, analgesic, antispasmodic, anti-inflammatory, diuretic and antiseptic activity. CONCLUSION: The patent registry survey demonstrates the possibility of beneficial action of matrix microparticles Copaifera langsdorffii on renal physiology, acting as antilithiasis and even with diuretic properties, thus opening up new possibilities of research of this substance on renal physiology

Efeito do microagulhamento na cicatriz de ferida cutânea induzida em ratos

Introdução: A saúde representa estado de completo bem-estar.As cicatrizes são exemplos de afecções que colocam em risco a integridade emocional do paciente, comprometendo sua qualidade de vida. Existem diversas alternativas terapêuticas visando amenizar distúrbios estéticos. O microagulhamento estimula a produção de colágeno, melhorando o efeito cicatricial da lesão provocada. Objetivo: avaliar o efeito do microagulhamento na cicatriz de ferida cutânea induzida cirurgicamente em ratos. Métodos: Foram utilizados 24 ratos, machos, distribuídos em cinco grupos de estudo. Em todos os grupos realizou-se a incisão cutânea induzida cirurgicamente no dorso do ani- mal, aguardando-se a cicatrização completa. Os grupos GC-14 e GC-30 foram controles para os grupos GCM-14 e GCM-30. Resultados: Foi identificada reepitelização e ausência de tecido de granulação em 100% dos grupos. Com relação à proporção de fibrose, observou-se redução média de 19% no grupo GCM-14 e de 4% no grupo GCM-30. Houve estímulo à produção de colágeno tipo I e III nos grupos submetidos ao microagulhamento, observando maior quantificação de colágeno tipo I no grupo GCM-14 (62,1%) em relação a seu controle (37,8%). Conclusões: o microagulhamento mostrou-se eficaz ao estimular maior produção de fibras colágenas em 14 dias, sugerindo tendência à melhora da cicatriz

Effects of hypertonic saline solution associated to remote ischemic perconditioning in kidney ischemia/reperfusion injury in rats

To evaluate the effects of hypertonic saline solution associated to remote ischemic perconditioning in renal ischemia/reperfusion injury in rats. Methods: Twenty five male rats (Wistar) underwent right nephrectomy and were distributed into five groups: Sham group (S); Ischemia/Reperfusion group (I/R) with 30 minutes of renal ischemia; Remote ischemic perconditioning group (Per) with three cycles of 10 minutes of I/R performed during kidney ischemia; Hypertonic saline solution group (HSS) treated with hypertonic saline solution (4ml/kg); remote ischemic perconditioning + Hypertonic saline solution group (Per+HSS) with both treatments. After reperfusion, blood samples were collected for BUN and creatinine serum levels analyzes. TBARS were evaluated in plasma and renal tissue to assess oxidative stress. Kidney histopathological examination were performed. Results: Per+HSS group showed a lower degree of renal dysfunction in relation to I/R group, whereas the technique of remote ischemic perconditioning isolated or associated with saline solution significantly reduced oxidative stress and histological damage. Conclusion:Remote ischemic perconditioning associated or not to saline solution promoted reduction of acute renal injury induced by ischemia/reperfusion.PERCÁRIO, S. Universidade Federal do Par

Efeito do óleo de copaíba associado ao microagulhamento na pele de ratos: um estudo comparativo

Introdução: O microagulhamento é técnica cuja finalidade é o estímulo do colágeno, bem como de drug delivery. O óleo de copaíba apresenta efeitos cicatrizantes e anti-inflamatórios que já foram demonstrados em vários modelos animais. Objetivo: Avaliar o efeito do óleo de copaíba associado ao microagulhamento na pele de ratos. Métodos: Foram utilizados 30 ratos distribuídos em seis grupos com cinco animais cada, submetidos a: microagulhamento isolado, microagulhamento associado a óleo mineral e microagulhamento associado a óleo de copaíba. Foram realizadas biópsias em todos os animais 14 dias e 30 dias após. Os parâmetros avaliados foram presença de colágeno, fibroblastos e vasos, classificada em ausente (0), leve (1), moderada (2) ou intensa (3). Resultados: Houve diferença estatisticamente significante entre os grupos em relação ao colágeno com 14 (p = 0,0091) e 30 dias (p = 0,0357) e fibroblastos com 30 dias (p = 0,0357). O grupo que utilizou microagulhamento e óleo de copaíba, apresentou, após 30 dias, maior produção de colágeno e de fibroblastos. Conclusões: o óleo de copaíba associado ao microagulhamento foi capaz de estimular maior produção de colágeno e de fibroblastos na pele de ratos

Perconditioning associated to hypertonic saline solution on liver function improvement after ischemia/reperfusion injury

<div><p>Abstract Purpose: To evaluate the effects of hypertonic saline solution associated to remote ischemic perconditioning in liver ischemia/reperfusion injury in rats. Methods: 25 male rats (Wistar) were distributed into five groups: Sham group (S); Ischemia/Reperfusion group (I/R) with 30 minutes of liver ischemia; Remote ischemic perconditioning group (Per) with three cycles of 10 minutes of I/R performed during liver ischemia; Hypertonic saline solution group (HSS) treated with hypertonic saline solution (4ml/kg); Remote ischemic perconditioning + Hypertonic saline solution group (Per+HSS) with both treatments. Results: Per+HSS group showed a lower degree of liver dysfunction in relation to I/R group, whereas the technique of remote ischemic perconditioning isolated or associated with saline solution significantly improved liver function and reduced histological damage. Conclusion: Remote ischemic perconditioning associated or not to saline solution promoted reduction of acute liver injury induced by ischemia/reperfusion.</p></div