Critical appraisal of systematic reviews of intervention studies in periodontology using AMSTAR 2 and ROBIS tools

Systematic reviews of intervention studies are used to support treatment recommendations. The aim of this study was to assess the methodological quality and risk of bias of systematic reviews of intervention studies in in the field of periodontology using AMSTAR 2 and ROBIS.Systematic reviews of randomized and non-randomized clinical trials, published between 2019 and 2020, were searched at MedLine, Embase, Web of Science, Scopus, Cochrane Library, LILACS with no language restrictions between October 2019 to October 2020. Additionally, grey literature and hand search was performed. Paired independent reviewers screened studies, extracted data and assessed the methodological quality and risk of bias through the AMSTAR 2 and ROBIS tools.One hundred twenty-seven reviews were included. According to AMSTAR 2, the methodological quality was mainly critically low (64.6%) and low (24.4%), followed by moderate (0.8%) and high (10.2%). According to ROBIS, 90.6% were at high risk of bias, followed by 7.1% low, and 2.4% unclear risk of bias. The risk of bias decreased with the increased in the impact factor of the journal.Current systematic reviews of intervention studies in periodontics were classified as low or critically low methodological quality and high risk of bias. Both tools led to similar conclusions. Better adherence to established reporting guidelines and stricter research practices when conducting systematic reviews are needed

Periodontal Therapy Effects on Nitrite Related to Oral Bacteria: A 6-Month Randomized Clinical Trial

Made available in DSpace on 2019-09-12T16:56:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2015Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Background: Nitrite is a biologic factor relevant to oral and systemic homeostasis. Through an oral bacteria reduction process, it was suggested that periodontal therapy and chlorhexidine (CHX) rinse could affect nitrite levels, leading to negative effects, such as an increase in blood pressure. This 6-month randomized clinical trial evaluated the effects of periodontal therapeutic protocols on salivary nitrite and its relation to subgingival bacteria. Methods: One hundred patients with periodontitis were allocated randomly to debridement procedures in four weekly sections (quadrant scaling [QS]) or within 24 hours (full-mouth scaling [FMS]) in conjunction with a 60-day CHX (QS + CHX and FMS + CHX), placebo (QS + placebo and FMS + placebo), or no mouthrinse (QS + none and FMS + none) use. Real-time polymerase chain reaction determined total bacterial, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Streptococcus oralis, and Actinomyces naeslundii levels. Salivary nitrite concentration was determined with Griess reagent. Data were analyzed statistically at baseline and 3 and 6 months by analysis of variance, Kruskal-Wallis, Mann-Whitney U, and Spearman correlation tests (P <0.05). Results: Nitrite concentrations did not tend to change over time. Regarding CHX use, there was a negative correlation between nitrite and total bacterial load at 6 months (FMS + CHX) and one positive correlation between P. gingivalis and nitrite at baseline (QS + CHX). Independently of rinse type, in the FMS group, nitrite correlated negatively with several microbial parameters and also with a higher percentage of deep periodontal pockets. Conclusions: The relationship between nitrite and bacterial levels appears weak. Short-term scaling exhibited a greater influence on nitrite concentrations then long-term CHX use.[Cortelli, Sheila C.; Cortelli, Jose R.] Universidade de Taubaté (Unitau), Sch Dent, Nucleus Periodontal Res, Sao Paulo, Brazil[Cortelli, Sheila C.] Univ Fed Minas Gerais, Sch Dent, Belo Horizonte, MG, Brazil[Costa, Fernando O.; Cota, Luis O. M.] Univ Fed Minas Gerais, Sch Dent, Periodontal Dept, Belo Horizonte, MG, Brazil[Rodrigues, Edson] Universidade de Taubaté (Unitau), Inst Biosci, Dept Biochem, Sao Paulo, Brazi

Gingival Overgrowth in Renal Transplant Subjects: A 44-Month Follow-Up Study

Made available in DSpace on 2019-09-12T16:53:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2013Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Background. To assess changes in gingival status of renal transplant subjects under immunosuppressive regimens based on cyclosporine (CsA), tacrolimus (Tcr), and sirolimus (Sir). Methods. From a sample of 135 subjects (baseline examination [BE]), 89 without periodontal treatment, who maintained the immunosuppressive regimen based on the same main agent (CsA=23, Tcr=31, and Sir=35), were reexamined after 44 months (follow-up examination [FE]). Demographic, pharmacologic, and periodontal variables were collected and gingival overgrowth (GO) was assessed by visual examination. Results. In Tcr and CsA groups, although not significant, occurrence of GO decreased (CsA [BE=56.5% and FE=34.8%; P=0.063] and Tcr [BE=19.4% and FE=12.9%; P=0.500]). In addition, the severity of GO decreased significantly in CsA group (mean score BE=10.29-7.70 and mean score FE=0.78-1.38; P=0.003). In Sir group, GO decreased from 17.1% (BE) to 0.0% (FE) (P=not applicable). In total sample, GO was associated with papillary bleeding index (P=0.001) and concomitant use of calcium channel blockers (P=0.029); in CsA and Tcr groups, GO was associated with papillary bleeding index (P=0.029 and 0.033, respectively). Conclusions. There was no incidence of GO, and a decrease in the occurrence and severity of GO was significant in total sample. This decrease can be attributed to changes in pharmacologic and periodontal variables over this period of time.[Costa, Lidiane C. M.; Costa, Fernando Oliveira; Cota, Luis O. M.] Univ Fed Minas Gerais, Sch Dent, Dept Periodontol, BR-31270901 Belo Horizonte, MG, Brazil[Cortelli, Sheila C.; Cortelli, Jose R.] Universidade de Taubaté (Unitau), Dept Dent, Periodont Res Di

Periodontal condition and levels of bacteria associated with periodontitis in individuals with bipolar affective disorders: A case-control study

Made available in DSpace on 2019-09-12T16:56:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2019Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig)Objective To evaluate the epidemiological and microbiological aspects of the potential association between bipolar affective disorder (BAPD) and periodontitis. Methodology The present case-control study comprised 176 individuals with BAPD and 176 controls. All individuals underwent a complete full-mouth periodontal examination and microbiological sampling. Data on bleeding on probing, probing depth, and clinical attachment level in all present teeth were recorded. Quantification of total bacterial load and Aggregatibacter actinomycetemcomitans, Treponema denticola, and Porphyromonas gingivalis counts were performed through qPCR. Data were analyzed using univariate analysis, Spearman correlation and multivariate logistic regression. Results The prevalence of periodontitis was 39.7% among controls and 58.5% among individuals with BAPD (OR = 2.13; 95% CI 1.39-3.27). A. actinomycetemcomitans and P. gingivalis counts were significantly higher in individuals with BPAD and periodontitis. The final multivariate logistic regression revealed that periodontitis was strongly associated with the total bacterial load (OR = 1.91; 95% IC = 1.0-1.99; P < 0.001) and the depressive phase of BPAD (OR = 28.94; 95% IC = 4.44-177.27; P < 0.001). Conclusion BAPD was associated with increased risk for periodontitis. Individuals with BPAD presented higher levels of A. actinomycetemcomitans and P. gingivalis, suggesting that periodontitis could be a co-morbidity frequently found in individuals with BAPD.[Cunha, Fabiano A.; Cota, Luis O. M.; Neves, Fernando S.; Costa, Fernando O.] Univ Fed Minas Gerais, Belo Horizonte, MG, Brazil[Cortelli, Sheila C.; Miranda, Tais B.; Cortelli, Jose R.] Universidade de Taubaté (Unitau

Risk variables in the association between frequency of alcohol consumption and periodontitis

Made available in DSpace on 2019-09-12T16:56:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2012Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Objective: Investigate the association between the frequency of alcohol consumption and periodontitis. Moreover, evaluate the influence of biological, behavioural, and social risk variables in this association. Methods: Sample was comprised by 542 subjects of both genders, 35-55 years of age, who underwent a complete periodontal examination, and was divided into four groups according to the frequency of alcohol use, based on alcohol use disorders identification test (AUDIT) and Cut-down, Annoyed, Guilty, Eye-opener (CAGE) instruments: (1) no or occasional alcohol use (NA), (2) moderate alcohol use (MA), (3) intense alcohol use (IA) and (4) alcohol dependence (DA). Associations between the occurrence of periodontitis and potential risk variables were analysed by univariate and multivariate logistic regression stratified by smoking status when appropriate. Results: The prevalence of periodontitis in NA, MA, IA and DA groups were 17.2%, 24.0%, 29.6% and 53%, respectively. Alcohol odds ratio (OR) estimates significantly increased with an increase in consumption frequency (DA>IA>MA>NA) and were approximately two times higher in smokers (OR = 3.43 to 7.91) compared to non-smokers (OR = 1.22 to 3.02). Conclusion: Occurrence of periodontitis among alcohol users were high and the frequency of alcohol consumption increased the odds of periodontitis incrementally mainly in smokers.[Lages, Eugenio J. P.; Costa, Fernando O.; Lages, Elizabeth M. B.; Cota, Luis O. M.; Cyrino, Renata M.] Univ Fed Minas Gerais, Dept Periodontol, Sch Dent, Antonio Carlos Ave 6627,Pampulha POB 359, BR-31270901 Belo Horizonte, MG, Brazil[Cortelli, Sheila C.; Nobre-Franco, Gilson C.; Cortelli, Jose R.] Universidade de Taubaté (Unitau), Dept Dent, Periodont Res Div, Sao Paulo, Brazi

Re: Alcohol Consumption and Periodontitis: Quantification of Periodontal Pathogens and Cytokines. Lages EJ, Costa FO, Cortelli SC, et al. (J Periodontol 2015; 86: 1058-1068.) Response

Made available in DSpace on 2019-09-12T16:56:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2016[Lages, Eugenio J. P.; Costa, Fernando O.; Cota, Luis O. M.; Cyrino, Renata Magalhaes; Lages, Elizabeth M. B.; Brito, Joao A. R.; Gomez, Ricardo S.] Univ Fed Minas Gerais, Fac Dent, Dept Dent Clin Oral Pathol & Dent Surg, Belo Horizonte, MG, Brazil[Cortelli, Sheila C.; Cortelli, Jose R.; Nobre-Franco, Gilson C.] Universidade de Taubaté (Unitau), Periodont Res Div, Dept Den

Alcohol Consumption and Periodontitis: Quantification of Periodontal Pathogens and Cytokines

Made available in DSpace on 2019-09-12T16:56:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2015Fundação de Amparo à Pesquisa do Estado de Minas Gerais (Fapemig)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Background: There are few studies on periodontal status related to microbiologic and immunologic profiles among individuals not or occasionally using alcohol and those with alcohol dependence. The aim of this study is to determine the effect of alcohol consumption on the levels of subgingival periodontal pathogens and proinflammatory cytokines (interleukin [IL]-1 beta and tumor necrosis factor [TNF]-alpha) in the gingival fluid among individuals with and without periodontitis. Methods: This observational analytic study includes 88 volunteers allocated in four groups (n = 22): individuals with alcohol dependence and periodontitis (ADP), individuals with alcohol dependence and without periodontitis (ADNP), individuals not or occasionally using alcohol with periodontitis (NAP), and individuals not or occasionally using alcohol without periodontitis (NANP). Levels of Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Prevotella intermedia, Eikenella corrodens, and Fusobacterium nucleatum were determined by real-time polymerase chain reaction on the basis of the subgingival biofilm, and IL-1 beta and TNF-alpha were quantified by enzyme-linked immunosorbent assay in gingival fluid samples. Results: Individuals with alcohol dependence showed worse periodontal status and higher levels of P. intermedia, E. corrodens, F. nucleatum, and IL-1 beta than non-users. No significant correlations between TNF-alpha and bacterial levels were observed. However, in the ADP group, higher levels of E. corrodens were correlated with higher levels of IL-1 beta. Conclusion: A negative influence of alcohol consumption was observed on clinical and microbiologic periodontal parameters, as well as a slight influence on immunologic parameters, signaling the need for additional studies.[Lages, Eugenio J. P.; Costa, Fernando O.; Cota, Luis O. M.; Cyrino, Renata Magalhaes; Lages, Elizabeth M. B.; Brito, Joao A. R.; Gomez, Ricardo S.] Univ Fed Minas Gerais, Dept Dent Clin Oral Pathol & Dent Surg, Fac Dent, BR-31270901 Belo Horizonte, MG, Brazil[Cortelli, Sheila C.; Cortelli, Jose R.; Nobre-Franco, Gilson C.] Universidade de Taubaté (Unitau), Periodont Res Div, Dept Dent, Sao Paulo, Brazi