The diameter of the acyclic Birkhoff polytope

In this work we give an interpretation of vertices and edges of the acyclic Birkhoff polytope, , where T is a tree with n vertices, in terms of graph theory. We generalize a recent result relatively to the diameter of the graph .http://www.sciencedirect.com/science/article/B6V0R-4R70RHM-1/1/4f38cb080e47b5fa8e0d6c36588d41a

Simulated Vaginal Fluid: Candida resistant strains biofilm characterization and vapor phase of essential oil effect

Introduction : Vulvovaginal candidiasis is a disease that affects millions of women worldwide. Oral formulations, topical creams or ointments are the conventional dosage forms, with an increase in drug administration through vaginal via. The use of simulated biological fluids (e.g. vaginal fluid) in the evaluation of antifungal therapies may better mimic the real biological environments and therefore provide a better understanding of the behavior of the antifungal. Methods : The main objective of this work was to compare planktonic growth and biofilm formation of Candida species, on common growth medium, Sabouraud Dextrose Broth (SDB) and on vaginal simulation conditions, Simulated Vaginal Fluid (SVF), through the optical density determination, colony-forming units and scanning electron microscopy. In addition, under the same conditions this study also evaluated the ability of vapor phase of oregano and white thyme essential oils (VP-EOs), potential alternative treatment, to inhibit biofilm formation and to destroy mature biofilms of vaginal isolates, through the colony-forming units determination. Results : Candida isolates maintained the same biofilm formation capacity and morphology in both media (SVF and SDB). Furthermore, the results obtained in this work related with VP-EOs effect agree with results acquired, previously, with SDB. This means that the effect of VP-EOs is not affected by the SVF medium, and that this fluid allows the dissolution of the volatile and bioactive compounds. Conclusions : These results can predict the in vivo behaviour, suggesting a potential effective application of VP-EOs as prophylactic or therapeutic treatment for biofilm-related vulvovaginal candidiasis.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and grant ref 2020.05720.BD for Liliana Fernandes. Also, this study was supported by LABBELS — Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020 and Maria Elisa Rodrigues thanks FCT for funding through program DL 57/2016—Norma transitóriainfo:eu-repo/semantics/publishedVersio

Utilization of waste materials to improve asphalt mixtures performance

This study aims to develop an innovative bitumen with large quantities of waste materials to improve asphalt mixtures performance. Different amounts of waste motor oil and waste HDPE were added to a new bitumen. The bitumen modified with 10% of waste motor oil and 5% of HDPE showed promising characteristics (high softening point temperatures and penetration slightly higher than the conventional bitumen). After the selection of the most promising modified bitumen, three asphalt mixtures were produced with different bitumens (namely conventional bitumen, commercial modified bitumen and the selected modified bitumen). Beyond that, this modified bitumen improved some mechanical characteristics of the asphalt mixture where it was used, in comparison to conventional and modified asphalt mixtures.Portuguese Government and EU/FSE within a PhD fellowship (SFRH/BD98379/2013) of the FCT, in the scope of POPH/QREN, by FEDER through the Competitiveness Factors Operational Programme (COMPETE) and by national funds through the Portuguese Foundation for Science and Technology (FCT) in the scope of PLASTIROAD Project (PTDC/ECM/119179/2010

Valorização de resíduos plásticos na modificação de betumes para pavimentos rodoviários

Com o aumento do tráfego rodoviário são colocadas maiores exigências aos pavimentos, surgindo a necessidade de melhorar o seu desempenho. Um método que pode melhorar significativamente a qualidade dos pavimentos flexíveis é a adição de polímeros aos ligantes/misturas betuminosas. Assim, foram produzidos ligantes modificados com polímeros reciclados, em diferentes condições, cujo desempenho foi comparado com o de ligantes convencionais e de um ligante modificado comercial. Os resultados dos ensaios laboratoriais serão utilizados na seleção de resíduos plásticos e das condições de produção do betume modificado para otimizar o seu comportamento, salientando-se que este estudo procura promover a reutilização de resíduos de uma forma mais ecológica e económica.Financiado por Fundos FEDER através do Programa Operacional Fatores de Competitividade – COMPETE e por Fundos Nacionais através da FCT – Fundação para a Ciência e a Tecnologia no âmbito do projeto PLASTIROADS (PTDC/ECM/119179/2010) e do projeto estratégico UI 4047 – 2011-2012

Synthesis, characterization and preliminary antibacterial evaluation against Staphylococcus aureus of a new 2,4,5-tri(hetero)arylimidazole derivative based on azaindole heterocycle

Imidazole derivatives are known for their numerous biological applications, such as antibacterial, antifungal, antioxidant, antiviral, antiparasitic and anticancer, among others. Therefore, several imidazole derivatives have been synthesized and developed in recent years as potential drugs in the treatment of several diseases. In this communication, we report the synthesis of a new imidazole derivative, substituted at positions 2, 4 and 5 with heterocyclic groups, using a simple synthetic methodology and an easy purification procedure. The new compound was characterized by the usual spectroscopic techniques (NMR, UV-Vis absorption and emission). The evaluation of the novel imidazole derivative as a potential antibiotic drug was carried out against the Gram-positive bacterium Staphylococcus aureus, using disk test diffusion method. Results showed a dose-response effect against the bacteria under study, revealing that the rational design of this imidazole derivative is quite promising to improve antibacterial activity of imidazole derivatives.This research was funded by the Foundation for Science and Technology (FCT) through CQ/UM (UIDB/00686/2020) and “Contrato‐Programa” UIDB/04050/2020 funded by national funds. Thanks are also due to FCT for financial support to the Portuguese NMR Network (PTNMR, Bruker Avance III 400‐Univ. Minho)info:eu-repo/semantics/publishedVersio

Antidepressants detection and quantification in whole blood samples by GC–MS/MS, for forensic purposes

Depression is among the most prevalent psychiatric disorders of our society, leading to an increase in antidepressant drug consumption that needs to be accurately determined in whole blood samples in Forensic Toxicology Laboratories. For this purpose, this work presents a new gas chromatography tandem mass spectrometry (GC–MS/MS) method targeting the simultaneous and rapid determination of 14 common Antidepressants in whole blood: 13 Antidepressants (amitriptyline, citalopram, clomipramine, dothiepin, fluoxetine, imipramine, mianserin, mirtazapine, nortryptiline, paroxetine, sertraline, trimipramine and venlafaxine) and 1 Metabolite (N-desmethylclomipramine). Solid-phase extraction was used prior to chromatographic separation. Chromatographic and MS/MS parameters were selected to improve sensitivity, peak resolution and unequivocal identification of the eluted analyte. The detection was performed on a triple quadrupole tandem MS in selected ion monitoring (SIM) mode in tandem, using electronic impact ionization. Clomipramine-D3 and trimipramine-D3 were used as deutered internal standards. The validation parameters included linearity, limits of detection, lower limit of quantification, selectivity/ specificity, extraction efficiency, carry-over, precision and robustness, and followed internationally accepted guidelines. Limits of quantification and detection were lower than therapeutic and subtherapeutic concentration ranges. Overall, the method offered good selectivity, robustness and quick response (<16 min) for typical concentration ranges, both for therapeutic and lethal levels.info:eu-repo/semantics/acceptedVersio

Towards honey authentication: Differentiation of Apis mellifera subspecies in European honeys based on mitochondrial DNA markers

Honey is the natural sweet substance produced by Apis mellifera honeybees in Europe. Depending on the country/region, the A. mellifera subspecies native to Europe belong to three different lineages: A (A. m. iberiensis), M (A. m. iberiensis and A. m. mellifera) and C (A. m. ligustica and A. m. carnica). In this work, two DNAbased approaches were developed with the aim of entomological authentication of European honeys. A cytb specific PCR assay was proposed to identify A-lineage honeybees, while a second method based on real-time PCR coupled to high resolution melting analysis targeting the COI gene was developed to differentiate C- and Mlineages honeybees. The proposed methodologies were validated successfully with honeys of known origin and applied to the entomological authentication of 20 commercial samples from different European countries. The results highlight the predominance of honeys from C-lineage honeybees in Europe, except in Iberian Peninsula countries (honey from A-lineage honeybees).The authors are grateful to Dora Henriques for assembling the mitogenomes and to Pilar de la Rua and António Pajuelo for supplying authentic honey samples. This work was supported by FCT (Fundação para a Ciência e Tecnologia) through project UID/QUI/50006/2013 – POCI/01/0145/FEDER/007265 with financial support from FCT/MEC through national funds and co-financed by FEDER, under the Partnership Agreement PT2020 and by the projects NORTE-01-0145- FEDER-000011 and BeeHappy – POCI-01-0145-FEDER-029871 (financed by FEDER through the COMPETE 2020 – Operational Programme for Competitiveness and Internationalisation (POCI) and FCT). S. Soares, L. Grazina and J. Costa are grateful to FCT grants (SFRH/BD/75091/2010, SFRH/BD/132462/2017 and SFRH/BPD/102404/2014, respectively) financed by POPH-QREN (subsidised by FSE and MCTES).info:eu-repo/semantics/publishedVersio

Estudo para produção de misturas com betume borracha a menores temperaturas

As misturas com betume borracha precisam de temperaturas mais elevadas no seu processo de fabrico e compactação devido à maior viscosidade deste ligante betuminoso modificado com borracha granulada de pneus usados. Caso contrário, o betume borracha poderá não garantir o correto envolvimento dos agregados e a necessária trabalhabilidade da mistura para a sua correta compactação. As consequências previsíveis relacionam-se com uma maior sensibilidade à água, o que reduz a durabilidade destas misturas e potencia a ocorrência de desagregações superficiais no pavimento. No entanto, o uso de tecnologias temperadas que permitem reduzir as temperaturas de produção e compactação das misturas betuminosas, pode ser uma solução mais sustentável para produção das misturas com betume borracha a menores temperaturas, desde que isso não afete o seu desempenho. Este trabalho consiste no estudo da utilização de uma dessas tecnologias para produzir misturas com betume borracha a menores temperaturas. Assim, estudou-se o efeito que a aplicação de um aditivo surfactante tem nas propriedades dos ligantes com e sem borracha. Em seguida estudou-se a redução de temperatura de produção. Por fim comparou-se o desempenho de misturas betuminosas com betume borracha normais e temperadas. No estudo concluiu-se que é possível reduzir bastante a temperatura de fabrico sem comprometer o desempenho da mistura com betume borracha

Thermohydrodynamic modelling of journal bearings under varying load angle and negative groove flow rate

The performance of hydrodynamic journal bearings is affected by the conditions under which the lubricant is fed to the bearing gap. Axial grooves are often used and, depending on their location relatively to the load line, they might substantially interfere with the hydrodynamic pressure generation and the thermal behaviour of the bearing. However, many of the existing tools for predicting bearing performance are not able to suitably predict bearing behaviour under varying load angle given the oversimplified way under which they treat lubricant feeding conditions. The present work proposes a detailed Thermohydrodynamic approach which realistically incorporates these conditions into the bearing analysis. Special care is put on the mass and energy-conserving models of the ruptured film region and on a detailed treatment of lubricant mixing within the vicinity of grooves. This includes the first full modelling of the effect of negative flow rate in a groove, a phenomenon originally described experimentally in detail by the authors in previous publications, and which happens for a broad range of load/groove angles. An extensive investigation on the influence of loading direction on the performance of twin groove journal bearings has been performed. This parameter is found to affect deeply all major performance parameters due to the interference of groove regions in the hydrodynamic pressure generation and in the flow rates at each groove.Fundação para a Ciência e a Tecnologia (FCT

Mechanical analysis of asphalt mixtures produced with waste plastic modified binders

This work compares the viscoelastic properties of an asphalt binder (70/100 pen) modified with different waste plastics and the mechanical properties of the resultant asphalt mixtures. Two different plastic wastes were used, namely recycled HDPE and EVA. Three different polymer modified binders were produced with these plastic wastes: i) 5% HDPE modified binder (P5); ii) 5% EVA modified binder (E5) and; iii) a modified binder with 4% of EVA and 2% HDPE (E4P2). Asphalt mixtures were produced with these modified binders, and their mechanical properties were analysed and compared with a conventional mixture produced with a 30/50 pen bitumen. It was possible to conclude that these recycled polymers are able to improve the mechanical performance of the asphalt mixtures used in road paving.FEDER funds through the Operational Programme for Competitiveness Factors & COMPETE and National Fund s through FCT & Foundation for Science and Technology under the project PLASTIROADS (PTDC/ECM/119179/2010) and Symposium_22 Recycling and Reuse of Wastes into New Composite Materials -2062-of the strategic project UI 4047- 2011-2012 Centre of Territory, Environment and Construction