Experimental evidence for adaptive divergence in response to a warmed habitat reveals roles for morphology, allometry, and parasite resistance

Ectotherms are expected to be particularly vulnerable to climate change–driven increases in temperature. Understanding how populations adapt to novel thermal environments will be key for informing mitigation plans. We took advantage of threespine stickleback (Gasterosteus aculeatus) populations inhabiting adjacent geothermal (warm) and ambient (cold) habitats to test for adaptive evolutionary divergence using a field reciprocal transplant experiment. We found evidence for adaptive morphological divergence, as growth (length change) in non-native habitats related to head, posterior and total body shape. Higher growth in fish transplanted to a non-native habitat was associated with morphological shape closer to native fish. The consequences of transplantation were asymmetric with cold sourced fish transplanted to the warm habitat suffering from lower survival rates and greater parasite prevalence than warm sourced fish transplanted to the cold habitat. We also found divergent shape allometries that related to growth. Our findings suggest that wild populations can adapt quickly to thermal conditions, but immediate transitions to warmer conditions may be particularly difficult

Bottlenose dolphin ecotypes of the western South Atlantic: the puzzle of habitats, coloration patterns and dorsal fin shapes

Phenotypic variations occur in several cetacean species, including common bottlenose dolphins Tursiops truncatus, which can also be distinguished as coastal and offshore ecotypes. In the western South Atlantic, these ecotypes have been described based on skeletal morphology and genetics. However, there is still no clear description to recognize them in the field. Here we searched for external diagnostic patterns that may facilitate their visual distinction and investigated their habitat use. We examined dorsal fin shapes and coloration of photo-identified dolphins distributed in a wide geographic range off the coast of southern and southeastern Brazil. A strong differentiation in the dorsal fin shape was observed, with a more falcate shape for offshore dolphins. We also found that offshore individuals have a darker color pattern, while coastal dolphins show 2 wider striped bands at the throat region and a longer rostrum, revealing that the ecotypes can be well distinguished in the field. We also detected differential habitat use. The coastal ecotype inhabits shallow waters (up to 18 m deep) close to the shore (up to 3 km). The offshore ecotype has a wider distribution and more flexible habitat use. It was usually found in coastal and deeper waters (maximum depth of 758 m and >200 km from the coast). Although we observed a small area of overlap in the distribution of the 2 ecotypes, both forms were not seen together. Therefore, our results reinforce the presence of a parapatric distribution and distinct morphology between the ecotypes, supporting their prior description as different subspecies

Sucesso adaptativo e percepções das perturbações hidrológicas por populações ribeirinhas em rios do semiárido brasileiro

Brazilian semi-arid streams are characterized by extreme hydrological events, with short periods of flooding and long periods of no surface water flow. Human populations living in the surroundings of these systems must be adapted to such hydrological disturbances. This paper evaluates the perceptions of riverine human populations in the Brazilian semi-arid, in relation to the hydrological disturbances, adaptations of such populations to withstand drought while maintaining social structure, and their utilization of the watershed during times of flooding. Data presented was collected from three riverine human populations that live near intermittent streams in the semi-arid region of Brazil. These populations rely mostly on the intermittent water supply from these systems and on the production from small-scaled agriculture and livestock. The surveyed populations maintain the stability of their social structures and organizations by producing an identification process through representations of hydrological disturbances (floods and drought). The resistance and resilience to these events basically involve strategies to avoid social ruptures, through the practice of subsistence agriculture, and the search for opportunities (e.g. adequate sites) to build weirs, wells and “cacimbas” (water holes). A simple yet well-structured scheme to utilize the watershed was found to be important during wet periods. The history of human adaptation to the Brazilian semi-arid depends on the configurations that both ecosystem and the social system take at each hydrological cycle. These aspects should be taken into account by decision-makers, and should be included in development-oriented policies adopted for the region. Any attempt to manage the semi-arid ecosystems should include the utilization of hydrological disturbances as part of the human element and its dimensions. Key words: riverine populations, survival strategies, flood, drought, stability, intermittent stream, semi-arid.Os rios do semiárido brasileiro são caracterizados por extremos hidrológicos, com curtos períodos de inundação e longos períodos de ausência de água. Populações humanas vivendo próximas a esses sistemas devem estar adaptadas a tais perturbações hidrológicas. Este trabalho avalia a percepção de populações ribeirinhas no semiárido brasileiro, considerando as perturbações hidrológicas, suas adaptações para resistir à seca enquanto mantêm a estrutura social e o uso da água durante os períodos de inundação. Este estudo foi realizado em três populações que vivem próximas a rios intermitentes na região semiárida do Brasil. Essas populações dependem principalmente da água de rios intermitentes para a agricultura em pequena escala e para a pecuária. As populações estudadas mantêm a estabilidade de suas estruturas e organizações sociais por meio das representações das perturbações hidrológicas (inundação e seca). A resistência e a resiliência a esses eventos envolvem basicamente estratégias para evitar rupturas sociais através da prática de agricultura de subsistência e pela procura de oportunidades (locais adequados) para construir açudes, poços e cacimbas. Um esquema simples e bem estruturado de utilização de água é também importante durante períodos de inundação. O histórico da adaptação humana no semiárido brasileiro resulta da conformação do ecossistema e do sistema social a cada ciclo hidrológico. Estes aspectos devem ser levados em conta por tomadores de decisões e devem ser considerados nas políticas que orientam o desenvolvimento dessa região. As ações de manejo nos ecossistemas do semiárido devem incluir a utilização das perturbações hidrológicas como parte do elemento humano e suas dimensões. Palavras-chave: populações ribeirinhas, estratégias de sobrevivência, inundação, seca, estabilidade, rio intermitente, semiárido

Testing the predictability of morphological evolution in contrasting thermal environments

Gaining the ability to predict population responses to climate change is a pressing concern. Using a “natural experiment,” we show that testing for divergent evolution in wild populations from contrasting thermal environments provides a powerful approach, and likely an enhanced predictive power for responses to climate change. Specifically, we used a unique study system in Iceland, where freshwater populations of threespine sticklebacks (Gasterosteus aculeatus) are found in waters warmed by geothermal activity, adjacent to populations in ambient-temperature water. We focused on morphological traits across six pairs from warm and cold habitats. We found that fish from warm habitats tended to have a deeper mid-body, a subterminally orientated jaw, steeper craniofacial profile, and deeper caudal region relative to fish from cold habitats. Our common garden experiment showed that most of these differences were heritable. Population age did not appear to influence the magnitude or type of thermal divergence, but similar types of divergence between thermal habitats were more prevalent across allopatric than sympatric population pairs. These findings suggest that morphological divergence in response to thermal habitat, despite being relatively complex and multivariate, are predictable to a degree. Our data also suggest that the potential for migration of individuals between different thermal habitats may enhance nonparallel evolution and reduce our ability to predict responses to climate change

The correct name of Lahille's bottlenose dolphin, Tursiops truncatus gephyreus Lahille, 1908

No abstract available

Offshore and coastal common bottlenose dolphins of the western South Atlantic face-to-face: what the skull and the spine can tell us

The taxonomy of Tursiops truncatus in the western South Atlantic is not resolved. Two different hypotheses have been proposed: (1) offshore and coastal ecotypes with a parapatric distribution, and (2) two species, T. truncatus and T. gephyreus, living in sympatry. To test these hypotheses, we examined a total of 100 physically mature skulls and 35 vertebral columns from the suggested overlap zone in southern Brazil. In all skulls, 24 measurements, four alveoli counts and two categorical variables were analyzed. Vertebral formula was determined and five measurements were taken from selected vertebrae. Multivariate analyses were conducted for skull and vertebral data. Results revealed the presence of two well‐separated groups. Specimens of Group1 had smaller skulls and shorter body lengths, but more vertebrae, than Group2. The morphological characteristics of each group corresponded well with two ecotypes of common bottlenose dolphins reported in other ocean basins. Therefore, we assigned the specimens of Group1 to the offshore ecotype, and Group2 to the coastal ecotype. Differences in the geographic locations and ratio of strandings supported the parapatric hypothesis. The significant morphological differentiation observed suggests the presence of different subspecies, but an additional independent line of evidence is needed to hypothesize whether they represent different species

Evaluation of free radical-scavenging and antihemolytic activities of quince (Cydonia oblonga) leaf: A comparative study with green tea (Camellia sinensis)

This study aimed to determine the phenolic profile and to investigate the antioxidant potential of quince (Cydonia oblonga) leaf, comparing it with green tea (Camellia sinensis). For these purposes, methanolic extracts were prepared and phenolics content of quince leaf was determined by HPLC/UV. The antioxidant properties were assessed by Folin–Ciocalteu reducing capacity assay and by the ability to quench the stable free radical 2,20-diphenyl-1-picrylhydrazyl (DPPH) and to inhibit the 2,20-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative hemolysis of human erythrocytes. 5-O-Caffeoylquinic acid was found to be the major phenolic compound in quince leaf extract. Quince leaf exhibited a significantly higher reducing power than green tea (mean value of 227.8 ± 34.9 and 112.5 ± 1.5 g/kg dry leaf, respectively). Quince leaf extracts showed similar DPPH radical-scavenging activities (EC50 mean value of 21.6 ± 3.5 lg/ml) but significantly lower than that presented by green tea extract (EC50 mean value of 12.7 ± 0.1 lg/ml). Under the oxidative action of AAPH, quince leaf methanolic extract significantly protected the erythrocyte membrane from hemolysis in a similar manner to that found for green tea (IC50 mean value of 30.7 ± 6.7 and 24.3 ± 9.6 lg/ml, respectively, P > 0.05). These results point that quince leaf may have application as preventive or therapeutic agent in diseases in which free radicals are involved

Ecological divergence and speciation in common bottlenose dolphins in the Western South Atlantic

Coastal and offshore ecotypes of common bottlenose dolphins have been recognized in the western South Atlantic, and it is possible that trophic niche divergence associated with social interactions is leading them to genetic and phenotypic differentiation. The significant morphological differentiation observed between these ecotypes suggests they represent two different subspecies. However, there is still a need to investigate whether there is congruence between morphological and genetic data to rule out the possibility of ecophenotypic variation accompanied by gene flow. Mitochondrial DNA (mtDNA) control region sequence data and 10 microsatellite loci collected from stranded and biopsied dolphins sampled in coastal and offshore waters of Brazil as well as 106 skulls for morphological analyses were used to determine whether the morphological differentiation was supported by genetic differentiation. There was congruence among the data sets, reinforcing the presence of two distinct ecotypes. The divergence may be relatively recent, however, given the moderate values of mtDNA nucleotide divergence (dA = 0.008), presence of one shared mtDNA haplotype and possibly low levels of gene flow (around 1% of migrants per generation). Results suggest the ecotypes may be in the process of speciation and reinforce they are best described as two different subspecies until the degree of nuclear genetic divergence is thoroughly evaluated: Tursiops truncatus gephyreus (coastal ecotype) and T. t. truncatus (offshore ecotype). The endemic distribution of T. t. gephyreus in the western South Atlantic and number of anthropogenic threats in the area reinforces the importance of protecting this ecotype and its habitat

Genetic divergence between two phenotypically distinct bottlenose dolphin ecotypes suggests separate evolutionary trajectories

Due to their worldwide distribution and occupancy of different types of environments, bottlenose dolphins display considerable morphological variation. Despite limited understanding about the taxonomic identity of such forms and connectivity among them at global scale, coastal (or inshore) and offshore (or oceanic) ecotypes have been widely recognized in several ocean regions. In the Southwest Atlantic Ocean (SWA), however, there are scarce records of bottlenose dolphins differing in external morphology according to habitat preferences that resemble the coastal-offshore pattern observed elsewhere. The main aim of this study was to analyze the genetic variability, and test for population structure between coastal (n = 127) and offshore (n = 45) bottlenose dolphins sampled in the SWA to assess whether their external morphological distinction is consistent with genetic differentiation. We used a combination of mtDNA control region sequences and microsatellite genotypes to infer population structure and levels of genetic diversity. Our results from both molecular marker types were congruent and revealed strong levels of structuring (microsatellites FST = 0.385, p < .001; mtDNA FST = 0.183, p < .001; ΦST = 0.385, p < .001) and much lower genetic diversity in the coastal than the offshore ecotype, supporting patterns found in previous studies elsewhere. Despite the opportunity for gene flow in potential “contact zones”, we found minimal current and historical connectivity between ecotypes, suggesting they are following discrete evolutionary trajectories. Based on our molecular findings, which seem to be consistent with morphological differentiations recently described for bottlenose dolphins in our study area, we recommend recognizing the offshore bottlenose dolphin ecotype as an additional Evolutionarily Significant Unit (ESU) in the SWA. Implications of these results for the conservation of bottlenose dolphins in SWA are also discussed.Organization for the Conservation of South American Aquatic Mammals-YAQU PACHA e.V., the Brazilian Long Term Ecological Program (PELD-National Council for Research and Technological Development/CNPq), Chevron Brasil Upstream Frade Ltda, BG Group, Brasil, Instituto Aqualie.http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)2045-7758am2018Mammal Research Institut