Electromagnetic KY production from the proton in a Regge-plus-resonance approach

A Regge-plus-resonance (RPR) description of the p(\gamma,K)Y and p(e,e'K)Y processes (Y = \Lambda, \Sigma^{0,+}) is presented. The proposed reaction amplitude consists of Regge-trajectory exchanges in the t channel, supplemented with a limited selection of s-channel resonance diagrams. The RPR framework contains a considerably smaller number of free parameters than a typical effective-Lagrangian model. Nevertheless, it provides an acceptable overall description of the photo- and electroproduction observables over an extensive photon energy range. It is shown that the electroproduction response functions and polarization observables are particularly useful for fine-tuning both the background and resonance parameters.Comment: 4 pages, 3 figures, Proceedings for IX International Conference on Hypernuclear and Strange Particle Physics (HYP2006), October 10-14 2006, Main

Quantitative proteomics analysis of the nuclear fraction of human CD4+ cells in the early phases of IL-4-induced Th2 differentiation

We used stable isotope labeling with 4-plex iTRAQ (isobaric tags for relative and absolute quantification) reagents and LC-MS/MS to investigate proteomic changes in the nucleus of activated human CD4(+) cells during the early stages of Th2 cell differentiation. The effects of IL-4 stimulation upon activated naïve CD4(+) cells were measured in the nuclear fractions from 6 and 24 h in three biological replicates, each using pooled cord blood samples derived from seven or more individuals. In these analyses, in the order of 800 proteins were detected with two or more peptides and quantified in three biological replicates. In addition to consistent differences observed with the nuclear localization/expression of established human Th2 and Th1 markers, there were changes that suggested the involvement of several proteins either only recently reported or otherwise not known in this context. These included SATB1 and among the novel changes detected and validated an IL-4-induced increase in the level of YB1. This unique data set from human cord blood CD4(+) T cells details an extensive list of protein determinations that compares with and complements previous data determined from the Jurkat cell nucleus.</p

Exclusive electroproduction of K+ Lambda and K+ Sigma^0 final states at Q^2 = 0.030-0.055 (GeV/c)^2

Cross section measurements of the exclusive p(e,e'K+)Lambda,Sigma^0 electroproduction reactions have been performed at the Mainz Microtron MAMI in the A1 spectrometer facility using for the first time the Kaos spectrometer for kaon detection. These processes were studied in a kinematical region not covered by any previous experiment. The nucleon was probed in its third resonance region with virtual photons of low four-momenta, Q^2= 0.030-0.055 (GeV/c)^2. The MAMI data indicate a smooth transition in Q^2 from photoproduction to electroproduction cross sections. Comparison with predictions of effective Lagrangian models based on the isobar approach reveal that strong longitudinal couplings of the virtual photon to the N* resonances can be excluded from these models.Comment: 16 pages, 7 figure

The gamma + p to K+ + Lambda and gamma + p to K+ + Sigma0 reactions at forward angles with photon energies from 1.5 to 2.4 GeV

Differential cross sections and photon beam asymmetries for the gamma p rightarrow K+ Lambda and gamma p rightarrow K+ Sigma0 reactions have been measured in the photon energy range from 1.5 GeV to 2.4 GeV and in the angular range from Theta_{cm} = 0 to 60 of the K+ scattering angle in the center of mass system at the SPring-8/LEPS facility. The photon beam asymmetries for both the reactions have been found to be positive and to increase with the photon energy. The measured differential cross sections agree with the data measured by the CLAS collaboration at cosTheta_{cm}<0.9 within the experimental uncertainties, but the discrepancy with the SAPHIR data for the K+Lambda reaction is large at cosTheta_{cm}>0.9. In the K+Lambda reaction, the resonance-like structure found in the CLAS and SAPHIR data at W=1.96 GeV is confirmed. The differential cross sections at forward angles suggest a strong K-exchange contribution in the t-channel for the K+Lambda reaction, but not for the K+Sigma0 reaction.Comment: 13 pages, 18 figures, submitted to Physical Review

The state of the art in the analysis of two-dimensional gel electrophoresis images

Software-based image analysis is a crucial step in the biological interpretation of two-dimensional gel electrophoresis experiments. Recent significant advances in image processing methods combined with powerful computing hardware have enabled the routine analysis of large experiments. We cover the process starting with the imaging of 2-D gels, quantitation of spots, creation of expression profiles to statistical expression analysis followed by the presentation of results. Challenges for analysis software as well as good practices are highlighted. We emphasize image warping and related methods that are able to overcome the difficulties that are due to varying migration positions of spots between gels. Spot detection, quantitation, normalization, and the creation of expression profiles are described in detail. The recent development of consensus spot patterns and complete expression profiles enables one to take full advantage of statistical methods for expression analysis that are well established for the analysis of DNA microarray experiments. We close with an overview of visualization and presentation methods (proteome maps) and current challenges in the field

"4D Biology for health and disease" workshop report

The "4D Biology Workshop for Health and Disease", held on 16-17th ofMarch 2010 in Brussels, aimed at finding the best organising principlesfor large-scale proteomics, interactomics and structural genomics/biology initiatives, and setting the vision for future high-throughputresearch and large-scale data gathering in biological and medical science.Major conclusions of the workshop include the following. (i)Development of new technologies and approaches to data analysis iscrucial. Biophysical methods should be developed that span a broadrange of time/spatial resolution and characterise structures andkinetics of interactions. Mathematics, physics, computational andengineering tools need to be used more in biology and new tools needto be developed. (ii) Database efforts need to focus on improveddefinitions of ontologies and standards so that system-scale data andassociated metadata can be understood and shared efficiently. (iii)Research infrastructures should play a key role in fosteringmultidisciplinary research, maximising knowledge exchange betweendisciplines and facilitating access to diverse technologies. (iv)Understanding disease on a molecular level is crucial. Systemapproaches may represent a new paradigm in the search for biomarkersand new targets in human disease. (v) Appropriate education andtraining should be provided to help efficient exchange of knowledgebetween theoreticians, experimental biologists and clinicians. Theseconclusions provide a strong basis for creating major possibilities inadvancing research and clinical applications towards personalisedmedicine.Biophysical Structural Chemistr