Companion planting to attract pollinators increases the yield and quality of strawberry fruit in gardens and allotments

1. Global pollinator declines have led to concern that crop yields might fall as a result of a pollination deficit. Companion planting is a traditional practice thought to increase yield of insect pollinated crops by planting a co-flowering species next to the crop. 2. Using a combination of conventional researcher-led experiments and observational citizen scientist data, we tested the effectiveness of bee-friendly borage (Borago officinalis) as a companion plant to strawberry (Fragaria x ananassa). Insect visitors to the ‘Test’ (strawberry + borage) versus ‘Control’ (strawberry only) plants were observed, and strawberry fruit collected. Strawberries collected during the researcher-led experiment were also subject to fruit measurements and assessments of market quality. 3. Companion plants were found to significantly increase both yield and market quality of strawberries, suggesting an increase in insect pollination per plant. Test strawberries companion planted with borage produced an average of 35% more fruits, and 32% increased yield by weight. Test strawberry plants produced significantly more fruit of higher aesthetic quality when assessed by Marketing Standards for Strawberries. 4. Although there was no significant difference in the overall insect visits, when broken down by broad insect group there were significantly more flies visiting the test strawberries than controls. 5. These results could have implications for both gardeners and commercial growers. As consumers prefer a cosmetically perfect fruit, the production of fruit with increased aesthetics aids food waste reduction

Factors influencing the initial establishment of salt marsh vegetation on engineered sea wall terraces in south east England

Sea walls provide vital flood protection for lowland coastal property. We investigated the integrity of a cost-effective method of repairing sea defences, which has potential to create habitat for coastal and salt marsh flora. Experimental stone-gabion and clay-filled terraces were installed as a soft engineered approach to repair damaged sea walls in estuarine embayments in south east England. Changes in the surface heights of sediment and vascular plant colonisation were monitored over a 22 month period. Seven of the 12 terraces were colonised, by 12 species of plant, reaching a maximum of 85% cover. The main drivers of plant colonisation were sediment stability, elevation, exposure and sediment shear strength. Terraces with least change in the surface height of sediments were favourable for plant colonisation. Ordination (Canonical Correspondence Analysis) showed 72% variation in plant distribution explained by elevation (37%), exposure (30%), terrace length and sediment shear strength (5%). Elevation was the most influential variable; recruitment increased as terrace height approached the height of existing marsh (r2 = 0.43). This cost-effective approach has the potential to provide protection to sea walls and create additional habitat for wildlife. Key considerations for the improvement of terrace design and construction are discussed

Early growth response genes 2 and 3 regulate the expression of Bcl6 and differentiation of T follicular helper cells

T follicular helper (Tfh) cells support differentiation of B cells to plasma cells and high affinity antibody production in germinal centers (GC) and Tfh differentiation requires the function of B cell lymphoma 6 (Bcl6). We have now discovered that early growth response gene (Egr) 2 and 3 directly regulate the expression of Bcl6 in Tfh cells which is required for their function in regulation of GC formation. In the absence of Egr2 and 3, the expression of Bcl6 in Tfh cells is defective leading to impaired differentiation of Tfh cells resulting in a failure to form GCs following virus infection and defects in production of anti-viral antibodies. Enforced expression of Bcl6 in Egr2/3 deficient CD4 T cells partially restored Tfh differentiation and GC formation in response to virus infection. Our findings demonstrate a novel function of Egr2/3 which is important for Tfh cell development and Tfh cell mediated B cell immune responses.This work was supported by Arthritis Research UK. The authors declare that they have no conflicts of interest with the contents of this article

Coordinated effects of sequence variation on DNA binding, chromatin structure, and transcription.

DNA sequence variation has been associated with quantitative changes in molecular phenotypes such as gene expression, but its impact on chromatin states is poorly characterized. To understand the interplay between chromatin and genetic control of gene regulation, we quantified allelic variability in transcription factor binding, histone modifications, and gene expression within humans. We found abundant allelic specificity in chromatin and extensive local, short-range, and long-range allelic coordination among the studied molecular phenotypes. We observed genetic influence on most of these phenotypes, with histone modifications exhibiting strong context-dependent behavior. Our results implicate transcription factors as primary mediators of sequence-specific regulation of gene expression programs, with histone modifications frequently reflecting the primary regulatory event

Modeling Farm Households' Price Responses in the Presence of Transaction Costs and Heterogeneity in Labor Markets

We develop a farm household model to analyze price responses of farm households. This model incorporates various types of transaction costs as well as labor heterogeneity. Nonproportional variable transaction costs or labor heterogeneity imply that production and consumption decisions become nonseparable, even when the household buys or sells labor. An empirical model is estimated using data from Midwest Poland. The results show that nonproportional variable transaction costs and labor heterogeneity significantly influence household behavior. Not all price elasticities, however, change significantly if these are neglected. Copyright 2007, Oxford University Press.