6 research outputs found
Validation of a UHPLC-FLD method for the simultaneous quantification of aflatoxins, ochratoxin A and zearalenone in barley
A fast and simple UHPLC-FLD method has been developed for the simultaneous
determination in barley of aflatoxins (B1, G1, B2 and G2), ochratoxin A (OTA) and
zearalenone (ZEA), some of the most important mycotoxins due to their toxicity and
occurrence. The procedure is based on the extraction of the six mycotoxins with a
mixture of acetonitrile and water, and the purification of the extract with
immunoaffinity columns before analysis. Detection of AFB1 and AFG1 is improved
using a photochemical reaction. The method has been validated with satisfactory results.
Limits of detection were 340 ng kg-1 for ZEA, 13 ng kg-1 for OTA and varied from 0.5
to 15 ng kg-1 for aflatoxins. Recovery percentages were between 78.2 and 109.2%. After
being validated, the method has been successfully applied to 20 barley samples
cultivated in a region of northern Spain (Navarra)
Responses of wood anatomy and carbon isotope composition of Quercus pubescens saplings subjected to two consecutive years of summer drought
Soroepidemiologia de Toxoplasma gondii em gatos domiciliados atendidos em clínicas particulares de Porto Alegre, RS, Brasil
¿Sarcoma de Kaposi agresivo asociado a la administración de esteroides o síndrome de reconstitución inmune?
The novel RUNX3/p33 isoform is induced upon monocyte-derived dendritic cell maturation and downregulates IL-8 expression
10.1016/j.imbio.2010.05.018Immunobiology2159-10812-82
Detecção de anticorpos para Toxoplasma gondii em soro de suínos criados e abatidos em frigoríficos da região da grande Porto Alegre-RS, Brasil Detection of antibodies against Toxoplasma gondii in sera from swine bred and slaughtered in the great Porto Alegre-RS abbattoirs, Brazil
No presente trabalho, objetivou-se contribuir com dados sobre a freqüência de sororeagentes para Toxoplasma gondii em suínos criados e abatidos na Região da Grande Porto Alegre e fornecer subsídios sobre a importância da transmissão deste protozoário, por suínos. Foram coletadas amostras de 240 suínos em frigoríficos da região. A freqüência de anticorpos anti- Toxoplasma gondii, determinada através da técnica de hemaglutinação indireta, foi de 20 % de soros iguais ou superiores a diluição 1:64. Na técnica de imunofluorescência indireta, foram encontrados 33,75% de soro com diluição iguais a 1:16 ou superiores.<br>This report objectived to contribuite with data about the antibodies occurence of Toxoplasma gondii in swine bred and slaughtered in the area of Great Porto Alegre- RS, Brazil. The data should supply with subsidies on the importance of this protozoan transmission through swines. Samples were taken from 240 swines at slaughterhouses in that region. The frequency of anti-Toxoplasma gondii antibodies, determinanet through the indirect hemagglutination technic was of 20% of serum equal or superior to 1:64 dilution. In the indirect immunofluorescence technic was found 33.75% in serum with a diluition equal to 1:16 or superior