hShroom1 links a membrane bound protein to the actin cytoskeleton.

hShroom1 (hShrm1) is a member of the Apx/Shroom (Shrm) protein family and was identified from a yeast two-hybrid screen as a protein that interacts with the cytoplasmic domain of melanoma cell adhesion molecule (MCAM). The characteristic signature of the Shrm family is the presence of a unique domain, ASD2 (Apx/Shroom domain 2). mRNA analysis suggests that hShrm1 is expressed in brain, heart, skeletal muscle, colon, small intestine, kidney, placenta and lung tissue, as well a variety of melanoma and other cell lines. Co-immunoprecipitation and bioluminescence resonance energy transfer (BRET) experiments indicate that hShrm1 and MCAM interact in vivo and by immunofluorescence microscopy some co-localization of these proteins is observed. hShrm1 partly co-localises with beta-actin and is found in the Triton X-100 insoluble fraction of melanoma cell extracts. We propose that hShrm1 is involved in linking MCAM to the cytoskeleton

Accidents at roadworks on all-purpose rural roads

SIGLEAvailable from British Library Document Supply Centre- DSC:3425.926(TRRL-CR--150) / BLDSC - British Library Document Supply CentreGBUnited Kingdo

Quantifying phenotypic plasticity of berry traits using an allometric-type approach: A case study on anthocyanins and sugars in berries of Cabernet Sauvignon

The definitive version is available at www.blackwell-synergy.comIn this paper we advance a novel allometric-type approach to quantify the stability of key berry traits viz. anthocyanins and sugars. To test the concept, we used data from Cabernet Sauvignon grown in a hot environment of South Australia. Sources of variation in berry traits included water supply, fruit load, seasonal conditions and their interactions. Anthocyanins and sugars were measured in berry samples taken 7–8 times between veraison and harvest. Rates and durations of accumulation of anthocyanins and sugars per berry were derived from a bi-linear model between amount of compound and thermal time. We develop a framework based on ‘α’ a parameter representing the slope of the regression between rate and duration in a log-log scale. This relationship accommodates three conditions viz. (a) potentially plastic, rate-driven trait (α –1), (b) potentially plastic, duration-driven trait (α < –1), and (c) a stable trait, whereby variation in rate and variation in duration cancel each other (α = –1). Under our experimental conditions, amount of anthocyanins (range of variation 148%) was more plastic than amount of sugars per berry (range of variation 37%). The slope α captured the differential plasticity of these traits: α was significantly greater than –1 for anthocyanins and statistically undistinguishable from –1 for sugars. The rate-dominated accumulation of anthocyanins explained the relatively large variation in this constituent whereas the tightly coupled, inverse relationship between duration and rate (α –1) explained the relative stability of sugars per berry. We conclude that our allometric-type relationship between rate and duration allows for the quantification of cultivar-environment specific plasticity of important berry traits.V.O. Sadras, R.M. Stevens, J.M. Pech, E.J. Taylor, P.R. Nicholas, M.G. McCarth

A surface plasmon resonance-based solution affinity assay for heparan sulfate-binding proteins

A surface plasmon resonance-based solution affinity assay is described for measuring the Kd of binding of heparin/heparan sulfate-binding proteins with a variety of ligands. The assay involves the passage of a pre-equilibrated solution of protein and ligand over a sensor chip onto which heparin has been immobilised. Heparin sensor chips prepared by four different methods, including biotin–streptavidin affinity capture and direct covalent attachment to the chip surface, were successfully used in the assay and gave similar Kd values. The assay is applicable to a wide variety of heparin/HS-binding proteins of diverse structure and function (e.g., FGF-1, FGF-2, VEGF, IL-8, MCP-2, ATIII, PF4) and to ligands of varying molecular weight and degree of sulfation (e.g., heparin, PI-88, sucrose octasulfate, naphthalene trisulfonate) and is thus well suited for the rapid screening of ligands in drug discovery applications

Influência do grão de sorgo como fonte de amido em ovinos alimentados com feno: parâmetros plasmáticos Influence of sorghum grain as a source of starch in sheep fed hay: plasma parameters

O objetivo deste trabalho experimental foi verificar a influência de diferentes níveis de grão de sorgo, como fonte de amido, nos parâmetros plasmáticos em ovinos alimentados com feno de capim-elefante (Pennisetum purpureum Schum). O sorgo foi utilizado em quatro níveis na dieta: 0, 15, 30 e 45%. Foram usados 12 ovinos machos castrados distribuídos em quatro tratamentos com três repetições. Foram coletadas seis amostras de sangue por animal logo antes da refeição da manhã (hora zero) e 1, 2, 3, 4, 6 e 8 horas após. O delineamento experimental foi o completamente casualizado. A 1ª hora após a refeição apresentou a maior concentração plasmática de uréia (53,3 mg/100 mL) e foi superior à 6ª e 8ª hora (49,5 e 49,3 mg/100 mL). A maior concentração de uréia no plasma coincidiu com a maior concentração de amônia no líquido ruminal. O tratamento com 30% de sorgo na dieta apresentou concentração plasmática de glicose de 81,0 mg/100 mL e foi superior ao tratamento testemunha (60,4 mg/100 mL). A concentração de insulina variou entre tratamentos para cada hora de coleta após a refeição, de acordo com os níveis de sorgo na dieta. O tratamento com 45% de sorgo apresentou a maior concentração do hormônio no plasma. Observou-se para todos os tratamentos um pico de produção do hormônio na 4ª hora após a refeição.<br>The objective of this experimental work was to evaluate the influence of different levels of sorghum grain, as a source of starch, on the plasma parameters of sheep fed elephant grass hay (Pennisetum purpureum Schum). Sorghum grain was included in the diet at four levels: 0, 15, 30 e 45%. Twelve castrated male sheep were assigned to four treatments with three replicates. Six samples of blood per animal were collected just before the morning feeding (zero hour) and after 1, 2, 3, 4, 6 and 8 hours. A completely randomized design was used. The first hour after feeding showed the highest plasma concentration of urea (53.3 mg/100 mL) and was higher than the six and eight-hour samples (49.5 and 49.3 mg/100 mL). The higher concentration of urea in the plasma coincided with the highest concentration of ammonia in the ruminal liquor. Treatment including 30% sorghum in the diet showed a glucose plasmatic concentration of 81,0 mg/100 mL and was superior to the control treatment (60.4 mg/100 mL). Insulin concentration varied among treatments for each hour of collection after feeding according to the levels of sorghum in the diet. The treatment that included 45% sorghum grain showed the highest concentration of this hormone in the plasma. It was observed a peak of the insulin production at the four-hour after feeding