A novel N-terminal extension in mitochondrial TRAP1 serves as a thermal regulator of chaperone activity.

Hsp90 is a conserved chaperone that facilitates protein homeostasis. Our crystal structure of the mitochondrial Hsp90, TRAP1, revealed an extension of the N-terminal β-strand previously shown to cross between protomers in the closed state. In this study, we address the regulatory function of this extension or 'strap' and demonstrate its responsibility for an unusual temperature dependence in ATPase rates. This dependence is a consequence of a thermally sensitive kinetic barrier between the apo 'open' and ATP-bound 'closed' conformations. The strap stabilizes the closed state through trans-protomer interactions. Displacement of cis-protomer contacts from the apo state is rate-limiting for closure and ATP hydrolysis. Strap release is coupled to rotation of the N-terminal domain and dynamics of the nucleotide binding pocket lid. The strap is conserved in higher eukaryotes but absent from yeast and prokaryotes suggesting its role as a thermal and kinetic regulator, adapting Hsp90s to the demands of unique cellular and organismal environments

Measurement of the Spatial Cross-Correlation Function of Damped Lyman Alpha Systems and Lyman Break Galaxies

We present the first spectroscopic measurement of the spatial cross-correlation function between damped Lyman alpha systems (DLAs) and Lyman break galaxies (LBGs). We obtained deep u'BVRI images of nine QSO fields with 11 known z ~ 3 DLAs and spectroscopically confirmed 211 R < 25.5 photometrically selected z > 2 LBGs. We find strong evidence for an overdensity of LBGs near DLAs versus random, the results of which are similar to that of LBGs near other LBGs. A maximum likelihood cross-correlation analysis found the best fit correlation length value of r_0 = 2.9^(+1.4)_(-1.5) h^(-1)Mpc using a fixed value of gamma = 1.6. The implications of the DLA-LBG clustering amplitude on the average dark matter halo mass of DLAs are discussed.Comment: 12 pages, 2 figures, accepted for publication in Astrophysical Journal Letter

Composting paper and grass clippings with anaerobically treated palm oil mill effluent

Purpose The purpose of this study is to investigate the composting performance of anaerobically treated palm oil mill effluent (AnPOME) mixed with paper and grass clippings. Methods Composting was conducted using a laboratory scale system for 40 days. Several parameters were determined: temperature, mass reduction, pH, electrical conductivity, colour, zeta potential, phytotoxicity and final compost nutrients. Results The moisture content and compost mass were reduced by 24 and 18 %, respectively. Both final compost pH value and electrical conductivity were found to increase in value. Colour (measured as PtCo) was not suitable as a maturity indicator. The negative zeta potential values decreased from −12.25 to −21.80 mV. The phytotoxicity of the compost mixture was found to decrease in value during the process and the final nutrient value of the compost indicates its suitability as a soil conditioner. Conclusions From this study, we conclude that the addition of paper and grass clippings can be a potential substrate to be composted with anaerobically treated palm oil mill effluent (AnPOME). The final compost produced is suitable for soil conditioner

Three-dimensional calculation of shuttle charging in polar orbit

The charged particles environment in polar orbit can be of sufficient intensity to cause spacecraft charging. In order to gain a quantitative understanding of such effects, the Air Force is developing POLAR, a computer code which simulates in three dimensions the electrical interaction of large space vehicles with the polar ionospheric plasma. It models the physical processes of wake generation, ambient ion collection, precipitating auroral electron fluxes, and surface interactions, including secondary electron generation and backscattering, which lead to vehicle charging. These processes may be followed dynamically on a subsecond timescale so that the rapid passage through intense auroral arcs can be simulated. POLAR models the ambient plasma as isotropic Maxwellian electrons and ions (0+, H+), and allows for simultaneous precipitation of power-law, energetic Maxwellian, and accelerated Gaussian distributions of electrons. Magnetic field effects will be modeled in POLAR but are currently ignored

A geometric proof of the Kochen-Specker no-go theorem

We give a short geometric proof of the Kochen-Specker no-go theorem for non-contextual hidden variables models. Note added to this version: I understand from Jan-Aake Larsson that the construction we give here actually contains the original Kochen-Specker construction as well as many others (Bell, Conway and Kochen, Schuette, perhaps also Peres).Comment: This paper appeared some years ago, before the author was aware of quant-ph. It is relevant to recent developments concerning Kochen-Specker theorem