Transiently Transfected Purine Biosynthetic Enzymes Form Stress Bodies

It has been hypothesized that components of enzymatic pathways might organize into intracellular assemblies to improve their catalytic efficiency or lead to coordinate regulation. Accordingly, de novo purine biosynthesis enzymes may form a purinosome in the absence of purines, and a punctate intracellular body has been identified as the purinosome. We investigated the mechanism by which human de novo purine biosynthetic enzymes might be organized into purinosomes, especially under differing cellular conditions. Irregardless of the activity of bodies formed by endogenous enzymes, we demonstrate that intracellular bodies formed by transiently transfected, fluorescently tagged human purine biosynthesis proteins are best explained as protein aggregation.This work was supported by grants from the United States National Institutes of Health, National Science Foundation, and Welch (F1515) and Packard Foundations to EMM. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Cellular and Molecular Biolog

Inter-decadal climate variability in the Southern Hemisphere: evidence from Tasmanian tree rings over the past three millennia

EXTRACT (SEE PDF FOR FULL ABSTRACT): The characterization of inter-decadal climate variability in the Southern Hemisphere is severely constrained by the shortness of the instrumental climate records. To help relieve this constraint, we have developed and analyzed a reconstruction of warm-season (November-April) temperatures from Tasmanian tree rings that now extends back to 800 BC. A detailed analysis of this reconstruction in the time and frequency domains indicates that much of the inter-decadal variability is principally confined to four frequency bands with mean periods of 31, 57, 77, and 200 years. ... In so doing, we show how a future greenhouse warming signal over Tasmania could be masked by these natural oscillations unless they are taken into account

A Changing Temperature Response With Elevation For Lagarostrobos Franklinii In Tasmania, Australia

A network of seven Huon pine ring-width chronologies is constructed from sites ranging in elevation from 200 to 950 metres above sea level in western Tasmania. The chronologies are analysed individually and collectively to explore Huon pine‘s response to climate as a function of elevation. Three chronologies from greater than 700 metres in elevation exhibit a strong, direct response to temperature for most growing season months (p<0.05), while three from below 700 metres exhibit a weaker, direct response to growing-season temperature, and a strong, inverse relationship with temperature of the prior season of growth, also significant at the 0.05 level. Moisture availability at these temperate rainforest sites is less growth-limiting than temperature, and significant correlations for January (inverse) and April (direct) of the year of growth largely reflect the inter-relationships between temperature, precipitation and cloudiness, and their combined influence on photosynthesis, particularly at higher-elevation sites. A rotated Principal Component Analysis reveals a clear grouping of the high and low-elevation chronologies, represented by the first and second eigenvectors, respectively. The 700 metre Lake Marilyn Low chronology is revealed to be a transitional site between the two groupings, and likely reflects an important climatic ecotone where both temperature and photosynthetically-active radiation drop below optimum levels for the species, and begin to directly inhibit growth. Tasmania's west coast climate has been shown to exhibit a distinct vertical structure, exemplified by a subsidence-inversion layer above 900 metres. Temperature increases slightly with altitude above 930 metres (the elevation at which a peak in daily minimum and maximum humidity levels is observed) before decreasing again. A dense, orographically-generated cloud-zone of reduced light and temperature has a mean altitude between 700 and 900 metres, with the steepest drop in both air and soil temperature exhibited between 850 and 930 metres. This structure can account for Huon pine‘s changing response to climate with elevation as described in this paper, and reinforces the importance of careful site selection for dendroclimatic research. In the case of reconstructing warm-season temperature from Tasmanian Huon pine, the desired signal might be maximised through sampling at the few rare, subalpine stands which have been located in western Tasmania. The great length afforded by the low-elevation Huon pine resource may ultimately yield a far more detailed reconstruction of regional climate throughout the Holocene, with respect to a vertical profile, following the development of more sound, mechanistically-based response models

TEXAS AGRICULTURE BY CONGRESSIONAL DISTRICT: 1993-1996

Texas Agriculture by Congressional Districts: 1993-96 is an update of previous editions of this publication. Commodity sales by county are estimates made by county agents and, therefore, are not official. In all but 4 of 30 Texas congressional districts, production agriculture accounts for over $100 million in sales. In 18 of the districts, farm program commodities represent over$50 million in sales.Production Economics,

Health and Financial Fragility: Evidence from Car Crashes and Consumer Bankruptcy

This paper assesses the importance of adverse health shocks as triggers of bankruptcy filings. We view car crashes as a proxy for health shocks and draw on a large sample of police crash reports linked to hospital admission records and bankruptcy case files. We report two findings: (i) there is a strong positive correlation between an individual\u27s pre-shock financial condition and his or her likelihood of suffering a health shock, an example of behavioral consistency; and (ii) after accounting for this simultaneity, we are unable to identify a causal effect of health shocks on bankruptcy filing rates. These findings emphasize the importance of risk heterogeneity in determining financial fragility, raise questions about prior studies of medical bankruptcy, and point to important challenges in identifying the triggers of consumer bankruptcy

Recent Increases in Tasmanian Huon Pine Ring Widths from a Subalpine Stand: Natural Climate Variability, CO2 Fertilisation, or Greenhouse Warming?

Tasmanian subalpine Huon pines from the extreme high-altitude limit of the species distribution provide a summer temperature reconstruction extending back beyond 800 Be. Compared to low elevation Huon pine sites, the subalpine ring-widths exhibit a straightforward direct response to current growth-season temperatures and indicate anomalous warming of 0.33 ± O.06°C from 1967- 91. This warming is consistent with Tasmanian instrumental records and with hemispheric and global records. The possibility that the trees are responding directly to CO2 fertilisation is explored, using a high-precision record of CO2, obtained from air in Antarctic ice and firn, plus direct measurements of air from Cape Grim. The temperature forcing appears capable of explaining the ring-width variations in the alpine trees over the full range of observed periods, whereas CO2 fertilisation would require a more complex interaction and is not supported by other arguments. Two millennia-long tree-ring reconstructions of summer temperatures from South America do not exhibit the recent warming, nor other features found in the Tasmanian record on decadal to century time-scales. In fact, the South American chronologies bear little resemblance to each other, but do, however, reflect their own regional instrumental records. The Mt Read ring-width chronology, and the instrumental temperature series used for its calibration, also co-vary with climate influences of a distinctly regional character, yet still replicate many of the features reported as hemispheric and global temperatures over the last century. Spectral analysis of the Mt Read tree-ring data over the full 2,792 years suggests that at least part of the recent warming in the instrumental records could be a consequence of "natural forcing" of the record, complicating an interpretation in terms of a greenhouse-forced warming

Paleoclimate histories improve access and sustainability in index insurance programs

Proxy-based climate reconstructions can extend instrumental records by hundreds of years, providing a wealth of climate information at high temporal resolution. To date, however, their usefulness for informing climate risk and variability in policy and social applications has been understudied. Here, we apply tree-ring based reconstructions of drought for the last 700 years in a climate index insurance framework to show that additional information from long climate reconstructions significantly improves our understanding of the underlying climate distributions and variability. We further show that this added information can be used to better characterize risk to insurance providers, in many cases providing meaningful reductions in long-term contract costs to farmers in stand-alone policies. The impact of uncertainty on insurance premiums can also be reduced when insurers diversify portfolios, and the availability of long-term climate information from tree rings across a broad geographic range provides an opportunity to characterize spatial correlation in climate risk across geographic regions. Our results are robust to the range of climate variability experienced over the last 400 years and in model simulations of the twenty-first century, even within the context of changing baselines due to low frequency variability and secular climate trends. These results demonstrate the utility of longer-term climate histories in index insurance applications. Furthermore, they make the case from a climate-variability perspective for the continued importance of such approaches to improving the instrumental climate record, even into a non-stationary climate future

Paleoclimate histories improve access and sustainability in index insurance programs

Proxy-based climate reconstructions can extend instrumental records by hundreds of years, providing a wealth of climate information at high temporal resolution. To date, however, their usefulness for informing climate risk and variability in policy and social applications has been understudied. Here, we apply tree-ring based reconstructions of drought for the last 700 years in a climate index insurance framework to show that additional information from long climate reconstructions significantly improves our understanding of the underlying climate distributions and variability. We further show that this added information can be used to better characterize risk to insurance providers, in many cases providing meaningful reductions in long-term contract costs to farmers in stand-alone policies. The impact of uncertainty on insurance premiums can also be reduced when insurers diversify portfolios, and the availability of long-term climate information from tree rings across a broad geographic range provides an opportunity to characterize spatial correlation in climate risk across geographic regions. Our results are robust to the range of climate variability experienced over the last 400 years and in model simulations of the twenty-first century, even within the context of changing baselines due to low frequency variability and secular climate trends. These results demonstrate the utility of longer-term climate histories in index insurance applications. Furthermore, they make the case from a climate-variability perspective for the continued importance of such approaches to improving the instrumental climate record, even into a non-stationary climate future

Links between Indo-Pacific climate variability and drought in the Monsoon Asia Drought Atlas

Drought patterns across monsoon and temperate Asia over the period 1877–2005 are linked to Indo-Pacific climate variability associated with the El Niño-Southern Oscillation (ENSO) and the Indian Ocean Dipole (IOD). Using the Monsoon Asia Drought Atlas (MADA) composed of a high-resolution network of hydroclimatically sensitive tree-ring records with a focus on the June–August months, spatial drought patterns during El Niño and IOD events are assessed as to their agreement with an instrumental drought index and consistency in the drought response amongst ENSO/IOD events. Spatial characteristics in drought patterns are related to regional climate anomalies over the Indo-Pacific basin, using reanalysis products, including changes in the Asian monsoon systems, zonal Walker circulation, moisture fluxes, and precipitation. A weakening of the monsoon circulation over the Indian subcontinent and Southeast Asia during El Niño events, along with anomalous subsidence over monsoon Asia and reduced moisture flux, is reflected in anomalous drought conditions over India, Southeast Asia and Indonesia. When an IOD event co-occurs with an El Niño, severe drought conditions identified in the MADA for Southeast Asia, Indonesia, eastern China and central Asia are associated with a weakened South Asian monsoon, reduced moisture flux over China, and anomalous divergent flow and subsidence over Indonesia. Insights into the relative influences of Pacific and Indian Ocean variability for Asian monsoon climate on interannual to decadal and longer timescales, as recorded in the MADA, provide a useful tool for assessing long-term changes in the characteristics of Asian monsoon droughts in the context of Indo-Pacific climate variability