Investigation of the influence of CO2 cryogenic coolant application on tool wear

The use of cryogenic coolants has emerged as an environmentally conscious alternative to emulsion coolant options. Cryogenic media can be delivered with a variety of methods to the cutting edge and they can be used in combination with other traditional coolant options such as Minimum Quantity Lubrication (MQL) and compressed air cooling in order to aid dissipation of heat generated in the cutting zone and maximize the lubrication of the cutting edge and thus prolong tool life. This study focuses on the investigation of tool life when milling aerospace grade titanium (Ti-6Al-4 V) under different coolant delivery options. Tool wear progression was recorded for the following coolant options: cryogenic CO 2 , emulsion flood cooling, dry machining, cryogenic CO 2 combined with air or MQL as well as MQL alone

Complex Coacervate Materials as Artificial Cells

Cells have evolved to be self-sustaining compartmentalized systems that consist of many thousands of biomolecules and metabolites interacting in complex cycles and reaction networks. Numerous subtle intricacies of these self-assembled structures are still largely unknown. The importance of liquid-liquid phase separation (both membraneless and membrane bound) is, however, recognized as playing an important role in achieving biological function that is controlled in time and space. Reconstituting biochemical reactions in vitro has been a success of the last decades, for example, establishment of the minimal set of enzymes and nutrients able to replicate cellular activities like the in vitro transcription translation of genes to proteins. Further than this though, artificial cell research has the aim of combining synthetic materials and nonliving macromolecules into ordered assemblies with the ability to carry out more complex and ambitious cell-like functions. These activities can provide insights into fundamental cell processes in simplified and idealized systems but could also have an applied impact in synthetic biology and biotechnology in the future. To date, strategies for the bottom-up fabrication of micrometer scale life-like artificial cells have included stabilized water-in-oil droplets, giant unilamellar vesicles (GUV’s), hydrogels, and complex coacervates. Water-in-oil droplets are a valuable and easy to produce model system for studying cell-like processes; however, the lack of a crowded interior can limit these artificial cells in mimicking life more closely. Similarly membrane stabilized vesicles, such as GUV’s, have the additional membrane feature of cells but still lack a macromolecularly crowded cytoplasm. Hydrogel-based artificial cells have a macromolecularly dense interior (although cross-linked) that better mimics cells, in addition to mechanical properties more similar to the viscoelasticity seen in cells but could be seen as being not dynamic in nature and limiting to the diffusion of biomolecules. On the other hand, liquid-liquid phase separated complex coacervates are an ideal platform for artificial cells as they can most accurately mimic the crowded, viscous, highly charged nature of the eukaryotic cytoplasm. Other important key features that researchers in the field target include stabilizing semipermeable membranes, compartmentalization, information transfer/communication, motility, and metabolism/growth. In this Account, we will briefly cover aspects of coacervation theory and then outline key cases of synthetic coacervate materials used as artificial cells (ranging from polypeptides, modified polysaccharides, polyacrylates, and polymethacrylates, and allyl polymers), finishing with envisioned opportunities and potential applications for coacervate artificial cells moving forward.</p

Tuning of Cationic Polymer Functionality in Complex Coacervate Artificial Cells for Optimized Enzyme Activity

Complex coacervates are a versatile platform to mimic the structure of living cells. In both living systems and artificial cells, a macromolecularly crowded condensate phase has been shown to be able to modulate enzyme activity. Yet, how enzyme activity is affected by interactions (particularly with cationic charges) inside coacervates is not well studied. Here, we synthesized a series of amino-functional polymers to investigate the effect of the type of amine and charge density on coacervate formation, stability, protein partitioning, and enzyme function. The polymers were prepared by RAFT polymerization using as monomers aminoethyl methacrylate (AEAM), 2-(dimethylamino)ethyl methacrylate (DMAEMA), imidazolepropyl methacrylamide (IPMAm), and [2-(methacryloyloxy)ethyl] trimethylammonium chloride (TMAEMA). Membranized complex coacervate artificial cells were formed with these polycations and an anionic amylose derivative. Results show that polycations with reduced charge density result in higher protein mobility in the condensates and also higher enzyme activity. Insights described here could help guide the use of coacervate artificial cells in applications such as sensing, catalysis, and therapeutic formulations.</p

Properties of Pt Schottky Type Contacts On High-Resistivity CdZnTe Detectors

In this paper we present studies of the I-V characteristics of CdZnTe detectors with Pt contacts fabricated from high-resistivity single crystals grown by the high-pressure Brigman process. We have analyzed the experimental I-V curves using a model that approximates the CZT detector as a system consisting of a reversed Schottky contact in series with the bulk resistance. Least square fits to the experimental data yield 0.78-0.79 eV for the Pt-CZT Schottky barrier height, and <20 V for the voltage required to deplete a 2 mm thick CZT detector. We demonstrate that at high bias the thermionic current over the Schottky barrier, the height of which is reduced due to an interfacial layer between the contact and CZT material, controls the leakage current of the detectors. In many cases the dark current is not determined by the resistivity of the bulk material, but rather the properties of the contacts; namely by the interfacial layer between the contact and CZT material.Comment: 12 pages, 11 figure

Relativistic Models for Binary Neutron Stars with Arbitrary Spins

We introduce a new numerical scheme for solving the initial value problem for quasiequilibrium binary neutron stars allowing for arbitrary spins. The coupled Einstein field equations and equations of relativistic hydrodynamics are solved in the Wilson-Mathews conformal thin sandwich formalism. We construct sequences of circular-orbit binaries of varying separation, keeping the rest mass and circulation constant along each sequence. Solutions are presented for configurations obeying an n=1 polytropic equation of state and spinning parallel and antiparallel to the orbital angular momentum. We treat stars with moderate compaction ((m/R) = 0.14) and high compaction ((m/R) = 0.19). For all but the highest circulation sequences, the spins of the neutron stars increase as the binary separation decreases. Our zero-circulation cases approximate irrotational sequences, for which the spin angular frequencies of the stars increases by 13% (11%) of the orbital frequency for (m/R) = 0.14 ((m/R) = 0.19) by the time the innermost circular orbit is reached. In addition to leaving an imprint on the inspiral gravitational waveform, this spin effect is measurable in the electromagnetic signal if one of the stars is a pulsar visible from Earth.Comment: 21 pages, 14 figures. A few explanatory sentences added and some typos corrected. Accepted for publication in Phys. Rev.