Multiple roles for protein kinase C in gastropod embryogenesis

Protein kinase C (PKC) contributes to the correct development of organisms, but its importance to the embryogenesis of molluscs is not yet known. We report here that PKC activation is cyclic within early developing embryos of the gastropod snail Lymnaea stagnalis, and that activation with phorbol myristate acetate (PMA) results in disorganised and developmentally arrested embryos within 24 h. Moreover, chronic modulation of PKC activation by PMA or by the PKC inhibitor GF109203X in early embryos results in altered rotation and gliding behaviours and heartbeat during development. Finally, dis-regulation of PKC activity during early development significantly increased the duration to hatching. Our findings thus support novel roles for PKC in L. stagnalis embryos, in several physiological contexts, providing further insights into the importance of protein kinases for gastropod development in general

Solving the Initial Value Problem of two Black Holes

We solve the elliptic equations associated with the Hamiltonian and momentum constraints, corresponding to a system composed of two black holes with arbitrary linear and angular momentum. These new solutions are based on a Kerr-Schild spacetime slicing which provides more physically realistic solutions than the initial data based on conformally flat metric/maximal slicing methods. The singularity/inner boundary problems are circumvented by a new technique that allows the use of an elliptic solver on a Cartesian grid where no points are excised, simplifying enormously the numerical problem.Comment: 4 pages, 3 figures. Minor corrections, some points clarified, and one reference added. To appear in Phys. Rev. Let

Global temperature definition affects achievement of long-term climate goals

The Paris Agreement on climate change aims to limit 'global average temperature' rise to 'well below 2 °C' but reported temperature depends on choices about how to blend air and water temperature data, handle changes in sea ice and account for regions with missing data. Here we use CMIP5 climate model simulations to estimate how these choices affect reported warming and carbon budgets consistent with the Paris Agreement. By the 2090s, under a low-emissions scenario, modelled global near-surface air temperature rise is 15% higher (5%-95% range 6%-21%) than that estimated by an approach similar to the HadCRUT4 observational record. The difference reduces to 8% with global data coverage, or 4% with additional removal of a bias associated with changing sea-ice cover. Comparison of observational datasets with different data sources or infilling techniques supports our model results regarding incomplete coverage. From high-emission simulations, we find that a HadCRUT4 like definition means higher carbon budgets and later exceedance of temperature thresholds, relative to global near-surface air temperature. 2 °C warming is delayed by seven years on average, to 2048 (2035-2060), and CO2 emissions budget for a >50% chance of <2 °C warming increases by 67 GtC (246 GtCO2)

Are the facial gender and facial age variants of the composite face illusion products of a common mechanism?

When the upper half of one face (‘target region’) is spatially aligned with the lower half of another (‘distractor region’), the two halves appear to fuse together perceptually, changing observers’ subjective perception of the target region. This ‘composite face illusion’ is regarded as a key hallmark of holistic face processing. Importantly, distractor regions bias observers’ subjective perception of target regions in systematic, predictable ways. For example, male and female distractor regions make target regions appear masculine and feminine; young and old distractor regions make target regions appear younger and older. In the present study, we first describe a novel psychophysical paradigm that yields precise reliable estimates of these perceptual biases. Next, we use this novel procedure to establish a clear relationship between observers’ susceptibility to the age and gender biases induced by the composite face illusion. This relationship is seen in a lab-based sample (N = 100) and replicated in an independent sample tested online (N = 121). Our findings suggest that age and gender variants of the composite illusion may be different measures of a common structural binding process, with an origin early in the face processing stream

Black Hole Data via a Kerr-Schild Approach

We present a new approach for setting initial Cauchy data for multiple black hole spacetimes. The method is based upon adopting an initially Kerr-Schild form of the metric. In the case of non-spinning holes, the constraint equations take a simple hierarchical form which is amenable to direct numerical integration. The feasibility of this approach is demonstrated by solving analytically the problem of initial data in a perturbed Schwarzschild geometry.Comment: 13 pages, RevTeX forma

Correia repeat enclosed elements and non-coding RNAs in the Neisseria species

Neisseria gonorrhoeae is capable of causing gonorrhoea and more complex diseases in the human host. Neisseria meningitidis is a closely related pathogen that shares many of the same genomic features and virulence factors, but causes the life threatening diseases meningococcal meningitis and septicaemia. The importance of non-coding RNAs in gene regulation has become increasingly evident having been demonstrated to be involved in regulons responsible for iron acquisition, antigenic variation, and virulence. Neisseria spp. contain an IS-like element, the Correia Repeat Enclosed Element, which has been predicted to be mobile within the genomes or to have been in the past. This repeat, present in over 100 copies in the genome, has the ability to alter gene expression and regulation in several ways. We reveal here that Correia Repeat Enclosed Elements tend to be near non-coding RNAs in the Neisseria spp., especially N. gonorrhoeae. These results suggest that Correia Repeat Enclosed Elements may have disrupted ancestral regulatory networks not just through their influence on regulatory proteins but also for non-coding RNAs

The collision of boosted black holes: second order close limit calculations

We study the head-on collision of black holes starting from unsymmetrized, Brill--Lindquist type data for black holes with non-vanishing initial linear momentum. Evolution of the initial data is carried out with the ``close limit approximation,'' in which small initial separation and momentum are assumed, and second-order perturbation theory is used. We find agreement that is remarkably good, and that in some ways improves with increasing momentum. This work extends a previous study in which second order perturbation calculations were used for momentarily stationary initial data, and another study in which linearized perturbation theory was used for initially moving holes. In addition to supplying answers about the collisions, the present work has revealed several subtle points about the use of higher order perturbation theory, points that did not arise in the previous studies. These points include issues of normalization, and of comparison with numerical simulations, and will be important to subsequent applications of approximation methods for collisions.Comment: 20 pages, RevTeX, 6 figures included with psfi

Identification of a major causative agent of human cercarial dermatitis, Trichobilharzia franki (Muller and Kimmig 1994), in southern England and its evolutionary relationships with other European populations.

Background Trichobilharzia is the most species rich and widely distributed genus of schistosomes and is known throughout Europe and North America as an agent of human cercarial dermatitis. The disease is caused by an acute allergic reaction in the skin that develops as a consequence of repeated contact with water containing schistosomatid cercariae. However, despite historical outbreaks of the disease, there are no published records of accurately identified Trichobilharzia species from the UK. Methods Two hundred Radix auricularia (L.) were sampled from a recreational fishing lake in Hampshire and emerging schistosomatid cercariae were collected for microscopy and DNA extraction. General morphological description of the cercariae was performed, alongside sequencing and phylogenetic analysis of the 28S ribosomal DNA for accurate species identification as well as comparisons of ITS1 in order to identify evolutionary affinities with other European populations. All molecular comparisons were performed using published sequences. Results The phylogenetic analysis of 28S sequences identified the cercariae as Trichobilharzia franki. Two unique British ITS1 haplotypes were identified which were most closely related to haplotypes of T. franki populations from France. Haplotype network analysis indicated the mixing of T. franki populations throughout Europe. It is suggested that parasite distribution is the probable result of the movement of migratory waterfowl. Conclusions This is the first accurate record of T. franki in the UK. The movement of T. franki with waterfowl could pose a considerable human health risk, as in mainland Europe, and signifies T. franki-associated human cercarial dermatitis as a re-emerging disease in the UK

Evolving the Bowen-York initial data for spinning black holes

The Bowen-York initial value data typically used in numerical relativity to represent spinning black hole are not those of a constant-time slice of the Kerr spacetime. If Bowen-York initial data are used for each black hole in a collision, the emitted radiation will be partially due to the ``relaxation'' of the individual holes to Kerr form. We compute this radiation by treating the geometry for a single hole as a perturbation of a Schwarzschild black hole, and by using second order perturbation theory. We discuss the extent to which Bowen-York data can be expected accurately to represent Kerr holes.Comment: 10 pages, RevTeX, 4 figures included with psfi

Calculation of wakefields in a 17 GHz beam-driven photonic band-gap accelerator structure

We present the theoretical analysis and computer simulation of the wakefields in a 17 GHz photonic band-gap (PBG) structure for accelerator applications. Using the commercial code CST Particle Studio, the fundamental accelerating mode and dipole modes are excited by passing an 18 MeV electron beam through a seven-cell traveling-wave PBG structure. The characteristics of the longitudinal and transverse wakefields, wake potential spectrum, dipole mode distribution, and their quality factors are calculated and analyzed theoretically. Unlike in conventional disk-loaded waveguide (DLW) structures, three dipole modes (TM[subscript 11]-like, TM[subscript 12]-like, and TM[subscript 13]-like) are excited in the PBG structure with comparable initial amplitudes. These modes are separated by less than 4 GHz in frequency and are damped quickly due to low radiative Q factors. Simulations verify that a PBG structure provides wakefield damping relative to a DLW structure. Simulations were done with both single-bunch excitation to determine the frequency spectrum of the wakefields and multibunch excitation to compare to wakefield measurements taken at MIT using a 17 GHz bunch train. These simulation results will guide the design of next-generation high-gradient accelerator PBG structures.United States. Dept. of Energy. High Energy Physics Division (Contract DEFG02- 91ER40648)China. Fundamental Research Funds for the Central Universities (Contract ZYGX 2010J055