A new method for automatic Multiple Partial Discharge Classification

A new wavelet based feature parameter have been developed to represent the characteristics of PD activities, i.e. the wavelet decomposition energy of PD pulses measured from non-conventional ultra wide bandwidth PD sensors such as capacitive couplers (CC) or high frequency current transformers (HFCT). The generated feature vectors can contain different dimensions depending on the length of recorded pulses. These high dimensional feature vectors can then be processed using Principal Component Analysis (PCA) to map the data into a three dimensional space whilst the first three most significant components representing the feature vector are preserved. In the three dimensional mapped space, an automatic Density-Based Spatial Clustering of Applications with Noise (DBSCAN) algorithm is then applied to classify the data cluster(s) produced by the PCA. As the procedure is undertaken in a three dimensional space, the obtained clustering results can be easily assessed. The classified PD sub-data sets are then reconstructed in the time domain as phase-resolved patterns to facilitate PD source type identification. The proposed approach has been successfully applied to PD data measured from electrical machines and power cables where measurements were undertaken in different laboratories

The Time of Flight System of the AMS-02 Space Experiment

The Time-of-Flight (TOF) system of the AMS detector gives the fast trigger to the read out electronics and measures velocity, direction and charge of the crossing particles. The new version of the detector (called AMS-02) will be installed on the International Space Station on March 2004. The fringing field of the AMS-02 superconducting magnet is $1.0\div2.5$ kG where the photomultiplers (PM) are installed. In order to be able to operate with this residual field, a new type of PM was chosen and the mechanical design was constrained by requiring to minimize the angle between the magnetic field vector and the PM axis. Due to strong field and to the curved light guides, the time resolution will be $150\div180$ ps, while the new electronics will allow for a better charge measurement.Comment: 5 pages, 4 figures. Proc. of 7th Int. Conf. on Adv. Tech. and Part. Phys., 15-19 October 2001,Como (Italy

Ultrastructural characterization of calcification onset and progression in subdermally implanted aortic valves. Histochemical and spectrometric data

Detailed characterization of the subdermal model is a significant tool for better understanding of calcification mechanisms occurring in heart valves. In previous ultrastructural investigation on six-week-implantated aortic valve leaflets, modified pre-embedding glutaraldehyde-cuprolinic-blue reactions (GA-CB) enabled sample decalcification with concurrent retention/staining of lipid-containing polyanionic material, which lined cells and cell-derived matrix-vesicle-like bodies (phthalocyanin-positive layers: PPLs) co-localizing with the earliest apatite nucleation sites. Additional post-embedding silver staining (GA-CB-S) revealed PPLs to contain calcium-binding sites. This investigation concerns valve leaflets subjected to shorter implantation times to shed light on the modifications associated with PPLs generation and calcification onset/progression. Spectrometric estimations revealed time-dependent calcium increase, for unreacted samples, and copper modifications indicating an increase in acidic, non-glycanic material, for GA-CB-reacted samples. Two-day-implant thin sections showed emission and subsequent reabsorption of lamellipodium-like protrusions by cells, originating ECM-containing vacuoles, and/or degeneration stages characterized by the appearance of GA-CB-S-reactive, organule-derived dense bodies and progressive dissolution of all cell membranes. In one-week-implants, the first PPL-lined cells were found to co-exist with cells where GA-CB-S-reactive material accumulated, or exudated towards their edges, or outcropped at the ECM milieu, so acquiring PPL features. PPL-derived material was observed increasingly to affect the ECM on thin sections of one-week- to six-week-implants. These results show an endogenous source for PPLs and reveal that a peculiar cascade of cell degenerative steps is associated with valve mineralization in the subdermal model, providing new useful parameters for more reliable comparison of this experimental calcification process versus the physiological and pathological processes

Effects of Long Term Hg Contamination on Soil Mercury Speciation and Soil Biological Activities.

The suspended matter discharged by the Isonzo river has carried over, for centuries, heavily contaminated mine spoils from the Idrija mercury mining site (Slovenija). A frequently flooded area at the confluence of the Isonzo and Torre rivers was chosen for a preliminary study on the effects of long term mercury pollution on soil biological activities, Hg speciation and plant bioavailability. Soil mercury contamination reached up to about 80 \ub5g g-1 near the banks of Isonzo river and decreased down to about 0.2 \ub5g g-1 near the Torre river, with the predominance of mercuric sulfide and elemental mercury. Soil microbial biomass was not adversely affected by Hg contamination as most soil biological activities, with the exception of arylsulphatase and acid phosphatase, which showed significant negative trends against total mercury and its fractions. Two plant genres (Arum spp. and Rubus spp.) were collected in four different places: Rubus spp. showed the largest uptake capacity of mercury (about 1 \ub5g g-1). Long term mercury contamination does not seem to constitute a stressing factor for soil biological activities but remains nevertheless a concern for its transfer through the food chain

The AMS-02 Time of Flight System. Final Design

The AMS-02 detector is a superconducting magnetic spectrometer that will operate on the International Space Station. The time of flight (TOF) system of AMS-02 is composed by four scintillator planes with 8, 8, 10, 8 counters each, read at both ends by a total of 144 phototubes. This paper describes the new design, the expected performances, and shows preliminary results of the ion beam test carried on at CERN on October 2002.Comment: 4 pages, 6 EPS figures. Proc. of the 28th ICRC (2003

Critical involvement of calcium-dependent cytosolic phospholipase A2\u3b1 in aortic valve interstitial cell calcification

The involvement of calcium-dependent cytosolic phospholipase A2\u3b1 (cPLA2\u3b1) in aortic valve calcification is not exhaustively elucidated. Here, cPLA2\u3b1 expression in aortic valve interstitial cell (AVIC) pro-calcific cultures simulating either metastatic or dystrophic calcification was estimated by qPCR, Western blotting, and counting of cPLA2\u3b1-immunoreactive cells, with parallel ultrastructural examination of AVIC calcific degeneration. These evaluations also involved pro-calcific AVIC cultures treated with cPLA2\u3b1 inhibitor dexamethasone. cPLA2\u3b1 over-expression resulted for both types of pro-calcific AVIC cultures. Compared to controls, enzyme content was found to increase by up to 300% and 186% in metastatic and dystrophic calcification-like cultures, respectively. Increases in mRNA amounts were also observed, although they were not as striking as those in enzyme content. Moreover, cPLA2\u3b1 increases were time-dependent and strictly associated with mineralization progression. Conversely, drastically lower levels of enzyme content resulted for the pro-calcific AVIC cultures supplemented with dexamethasone. In particular, cPLA2\u3b1 amounts were found to decrease by almost 88% and 48% in metastatic and dystrophic calcification-like cultures, respectively, with mRNA amounts showing a similar trend. Interestingly, these drastic decreases in cPLA2\u3b1 amounts were paralleled by drastic decreases in mineralization degrees, as revealed ultrastructurally. In conclusion, cPLA2\u3b1 may be regarded as a crucial co-factor contributing to AVIC mineralization in vitro, thus being an attractive potential target for designing novel therapeutic strategies aimed to counteract onset or progression of calcific aortic valve diseases

How resistant are levodopa-resistant axial symptoms? Response of freezing, posture, and voice to increasing levodopa intestinal infusion rates in Parkinson disease

Background and purpose: Treatment of freezing of gait (FoG) and other Parkinson disease (PD) axial symptoms is challenging. Systematic assessments of axial symptoms at progressively increasing levodopa doses are lacking. We sought to analyze the resistance to high levodopa doses of FoG, posture, speech, and altered gait features presenting in daily-ON therapeutic condition. Methods: We performed a pre-/postinterventional study including patients treated with levodopa/carbidopa intestinal gel infusion (LCIG) with disabling FoG in daily-ON condition. Patients were evaluated at their usual LCIG infusion rate (T1), and 1 h after 1.5× (T2) and 2× (T3) increase of the LCIG infusion rate by quantitative outcome measures. The number of FoG episodes (primary outcome), posture, speech, and gait features were objectively quantified during a standardized test by a blinded rater. Changes in motor symptoms, dyskinesia, and plasma levodopa concentrations were also analyzed. Results: We evaluated 16 patients with a mean age of 69 ± 9.4 years and treated with LCIG for a mean of 2.2 ± 2.1 years. FoG improved in 83.3% of patients by increasing the levodopa doses. The number of FoG episodes significantly decreased (mean = 2.3 at T1, 1.7 at T2, 1.2 at T3; p = 0.013). Posture and speech features did not show significant changes, whereas stride length (p = 0.049), turn duration (p = 0.001), and turn velocity (p = 0.024) significantly improved on doubling the levodopa infusion rate. Conclusions: In a short-term evaluation, the increase of LCIG dose can improve "dopa-resistant" FoG and gait issues in most advanced PD patients with overall good control of motor symptoms in the absence of clinically significant dyskinesia