67 research outputs found
Subsurface processes generated by bore-driven swash on coarse-grained beaches
Peer reviewedPublisher PD
Live Tissue Imaging Shows Reef Corals Elevate pH under Their Calcifying Tissue Relative to Seawater
The threat posed to coral reefs by changes in seawater pH and carbonate chemistry (ocean acidification) raises the need for a better mechanistic understanding of physiological processes linked to coral calcification. Current models of coral calcification argue that corals elevate extracellular pH under their calcifying tissue relative to seawater to promote skeleton formation, but pH measurements taken from the calcifying tissue of living, intact corals have not been achieved to date. We performed live tissue imaging of the reef coral Stylophora pistillata to determine extracellular pH under the calcifying tissue and intracellular pH in calicoblastic cells. We worked with actively calcifying corals under flowing seawater and show that extracellular pH (pHe) under the calicoblastic epithelium is elevated by ā¼0.5 and ā¼0.2 pH units relative to the surrounding seawater in light and dark conditions respectively. By contrast, the intracellular pH (pHi) of the calicoblastic epithelium remains stable in the light and dark. Estimates of aragonite saturation states derived from our data indicate the elevation in subcalicoblastic pHe favour calcification and may thus be a critical step in the calcification process. However, the observed close association of the calicoblastic epithelium with the underlying crystals suggests that the calicoblastic cells influence the growth of the coral skeleton by other processes in addition to pHe modification. The procedure used in the current study provides a novel, tangible approach for future investigations into these processes and the impact of environmental change on the cellular mechanisms underpinning coral calcification
The skeleton of the staghorn coral Acropora millepora: molecular and structural characterization
15 pagesInternational audienceThe scleractinian coral Acropora millepora is one of the most studied species from the Great Barrier Reef. This species has been used to understand evolutionary, immune and developmental processes in cnidarians. It has also been subject of several ecological studies in order to elucidate reef responses to environmental changes such as temperature rise and ocean acidification (OA). In these contexts, several nucleic acid resources were made available. When combined to a recent proteomic analysis of the coral skeletal organic matrix (SOM), they enabled the identification of several skeletal matrix proteins, making A. millepora into an emerging model for biomineralization studies. Here we describe the skeletal microstructure of A. millepora skeleton, together with a functional and biochemical characterization of its occluded SOM that focuses on the protein and saccharidic moieties. The skeletal matrix proteins show a large range of isoelectric points, compositional patterns and signatures. Besides secreted proteins, there are a significant number of proteins with membrane attachment sites such as transmembrane domains and GPI anchors as well as proteins with integrin binding sites. These features show that the skeletal proteins must have strong adhesion properties in order to function in the calcifying space. Moreover this data suggest a molecular connection between the calcifying epithelium and the skeletal tissue during biocalcification. In terms of sugar moieties, the enrichment of the SOM in arabinose is striking, and the monosaccharide composition exhibits the same signature as that of mucus of acroporid corals. Finally, we observe that the interaction of the acetic acid soluble SOM on the morphology of in vitro grown CaCO3 crystals is very pronounced when compared with the calcifying matrices of some mollusks. In light of these results, we wish to commend Acropora millepora as a model for biocalcification studies in scleractinians, from molecular and structural viewpoints
Microstructure and biomechanical characteristics of bone substitutes for trauma and orthopaedic surgery
Abstract.
BACKGROUND: Many (artificial) bone substitute materials are currently available for use in orthopaedic trauma surgery. Objective data on their biological and biomechanical characteristics, which determine their clinical application, is mostly lacking. The aim of this study was to investigate structural and in vitro mechanical properties of nine bone substitute cements registered for use in orthopaedic trauma surgery in the Netherlands.
METHODS: Seven calcium phosphate cements (BoneSourceĀ®, CalcibonĀ®, ChronOSĀ®, EuroboneĀ®, HydroSetā¢, Norian SRSĀ®, and OstimĀ®), one calcium sulphate cement (MIIGĀ® X3), and one bioactive glass cement (CortossĀ®) were tested. Structural characteristics were measured by micro-CT scanning. Compression strength and stiffness were determined following unconfined compression tests.
RESULTS: Each bone substitute had unique characteristics. Mean total porosity ranged from 53% (OstimĀ®) to 0.5% (Norian SRSĀ®). Mean pore size exceeded 100 Ī¼m only in EuroboneĀ® and CortossĀ® (162.2 Ā± 107.1 Ī¼m and 148.4 Ā± 70.6 Ī¼m, respectively). However, 230 Ī¼m pores were found in CalcibonĀ®, Norian SRSĀ®, HydroSetā¢, and MIIGĀ® X3. Connectivity density ranged from 27/cm3 for HydroSetā¢ to 0.03/cm3 for CalcibonĀ®. The ultimate compression strength was highest in CortossĀ® (47.32 MPa) and lowest in OstimĀ® (0.24 MPa). Young's Modulus was highest in CalcibonĀ® (790 MPa) and lowest in OstimĀ® (6 MPa).
CONCLUSIONS: The bone substitutes tested display a wide range in structural properties and compression strength, indicating that they will be suitable for different clinical indications. The data outlined here will help surgeons to select the most suitable products currently available for specific clinical indications
Descargas discretas de Ć”gua subterrĆ¢nea em zonas hĆŗmidas identificadas com um modelo tridimensional de temperatura e com indicadores botĆ¢nicos (Boxford, Reino Unido)
Using a Thermal Proxy to Examine SedimentāWater Exchange in Mid-Continental Shelf Sandy Sediments
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