1,920 research outputs found

    A pictorial record of the program of waterfront activities at Agassiz Village, an organizational type boys' camp in West Poland, Maine.

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    Thesis (M.Ed.)--Boston University 78 Kadachrome slides accompany thesis and may be found in the Audio-visual Library. N.B. Table of contents says summary is on page 18 but it is found on page 14

    When and where? Pathogenic Escherichia coli differentially sense host D-serine using a universal transporter system to monitor their environment

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    Sensing environmental stimuli is critically important for bacteria when faced with the multitude of adversities presented within the host. Responding appropriately to these signals and in turn integrating these responses into the regulatory network of the cell allows bacteria to control precisely when and where they should establish colonization. D-serine is an abundant metabolite of the human urinary tract but is a toxic metabolite for Escherichia coli that lack a D-serine tolerance locus. Enterohaemorrhagic E. coli (EHEC) cannot catabolize D-serine for this reason and colonize the large intestine specifically, an environment low in D-serine. EHEC can however use D-serine sensing to repress colonization thus signaling the presence of an unfavorable environment. In our recent work (Connolly, et al. PLoS Pathogens (2016) 12(1): e1005359), we describe the discovery of a functional and previously uncharacterized D-serine uptake system in E. coli. The genes identified are highly conserved in all E. coli lineages but are regulated differentially in unique pathogenic backgrounds. The study identified that EHEC, counter-intuitively, increase D-serine uptake in its presence but that this is a tolerated process and is used to increase the transcriptional response to this signal. It was also found that the system has been integrated into the transcriptional network of EHEC-specific virulence genes, demonstrating an important pathotype-specific adaptation of core genome components

    Tracking elusive cargo: Illuminating spatio-temporal type 3 effector protein dynamics using reporters

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    Type 3 secretion systems (T3SS) form an integral part of the arsenal of many pathogenic bacteria. These injection machines, together with their cargo of subversive effector proteins are capable of manipulating the cellular environment of the host in order to ensure persistence of the pathogen. In order to fully appreciate the functions of Type 3 effectors it is necessary to gain spatio-temporal knowledge of each effector during the process of infection. A number of genetic modifications have been exploited in order to reveal effector protein secretion, translocation and subsequent activity and localisation within host cells. In this review, we will discuss the many available approaches for tracking effector protein dynamics and discuss the challenges faced to improve the current technologies and gain a clearer picture of effector protein function

    Affectivity and job satisfaction: a meta-analysis

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    The goal of this investigation was to examine the affective determinants of job satisfaction. Correlations between affectivity and job satisfaction measures were examined by cumulating research findings across studies. Measurement of affectivity in this study focused on five constructs, (1) negative affectivity, (2) positive affectivity, (3) affective disposition, (4) positive & negative affectivity, (5) all affectivity measures combined. The correlations between these five constructs and job satisfaction were meta-analyzed. The mean correlation corrected for coefficient alpha in both the affectivity and job satisfaction measures were: .49 for positive affectivity (N= 3,326, k= 15), -. 33 for negative affectivity (N= 6,028, k= 25), .36 for affective disposition (N= 1,415, k= 7), .39 for positive & negative affectivity (N= 9,354, k= 40), and .38 for all measures of affectivity combined (N= 10,769, k= 47). Results indicated that 10% - 25% of variance in job satisfaction could be due to individual differences in affectivity. No strong moderator variables were found. Implications for a Dispositional and situational source of job satisfaction are discussed

    From Jacobs to the Just City: A foundation for challenging the green planning orthodoxy

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    Unidad de excelencia María de Maeztu MdM-2015-0552Now that Jane Jacobs' ideas are seen as urban planning orthodoxy, it is unclear how her institutional goal of progressive change for the field will carry forward. In the 1960s, Jacobs created the conditions for institutional change by offering a thorough critique of the "Radiant Garden City Beautiful" orthodoxy of urban planning and presenting a solution for the problems that she saw with this approach. She argued that the top-down, design-oriented planning of her time hurt the lives of individual residents and diminished society as a whole. Her solution was a new way of seeing the city: as a functional and efficient social system. Since the 1990s, a global planning orthodoxy - of which Jacobs' ideas are part - developed around the "Smart Sustainable Resilient City." This orthodoxy has been subject to critique, but Susan Fainstein's Just City theory offers tools for comprehensively challenging the approach and a solution for addressing the problems. In order to demonstrate the need for institutional change within the Smart Sustainable Resilient City orthodoxy, I use the Just City theoretical perspective to interpret the results of an analysis of green gentrification in New York City between 1990 and 2014. I argue that the over-valuation of Jacobsian diversity within the current urban planning orthodoxy generates unjust outcomes. The just green city, then, requires de-emphasizing Jacobs' intellectual project in favor of her far more important institutional project

    Three Histories of Greening and Whiteness in American Cities

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    Unidad de excelencia María de Maeztu CEX2019-000940-MHow has urban greening related to the degree of whiteness in neighborhoods? The answer to this question provides an essential "historical diagnostic" that can be used to develop an approach to urban ecology which integrates racial and ethnic change into the planning for proposed interventions. In this paper we employ state sequence analysis to analyze the historical trend of greening (including the implementation of new parks, greenways, community gardens, green recreation areas, and nature preserves) between 1975 and 2014 in a sample of nine cities in the United States relative to concentrations of white and non-white residents. We divide the nine cities into three common growth trajectories and separately examine the trends for each growth trajectory. We further illustrate these trends by mobilizing qualitative data from field work in selected neighborhoods to help explain the processes that generate certain key findings in the quantitative data. We find that the relationship between greening and race/ethnicity differs according to city-level growth trajectory. Cities with continuous high and rapid levels of growth in the postwar period have the strongest link between increased greening and whiter populations. Meanwhile, in cities that contracted or had a punctuated growth pattern, non-white areas had a uniformly low level of greening that occurred mostly in recent years. In all, we show how urban growth, greening, and whiteness are inextricably associated qualities of American cities. We argue that understanding this association is essential for development of a race-conscious model for enhancing urban ecosystems

    A bin-microphysics parcel model investigation of secondary ice formation in an idealised shallow convective cloud

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    We provide the first systematic study of ice formation in idealised shallow clouds from collisions of supercooled water drops with ice particles (&lsquo;mode 2&rsquo;). Using the University of Manchester bin-microphysics parcel model, we investigated the sensitivity of ice formation due to mode 2 for a wide range of parameters: aerosol particle size distribution, updraft speed, cloud base temperature, cloud depth, ice-nucleating particle concentration and freezing fraction of mode 2. We provide context to our results with other secondary ice production mechanisms as single mechanisms and combinations (rime-splintering, spherical freezing fragmentation of drops [&lsquo;mode 1&rsquo;] and ice-ice collisions). There was a significant sensitivity to aerosol particle size distribution when updraft speeds were low (0.5 m s&minus;1); secondary ice formation did not occur when the aerosol particle size distribution mimicked polluted environments. Where secondary ice formation did occur in simulated clouds, significant ice formation in the shallower clouds (1.3 km deep) was due to mode 2 or a combination which included mode 2. The deeper clouds (2.4 km deep) also had significant contributions from rime-splintering or ice-ice collisions SIP mechanisms. While simulations with cloud base temperatures of 7 &deg;C were relatively insensitive to ice-nucleating particle concentrations, there was a sensitivity in simulations cloud base temperatures of 0 &deg;C. Increasing the ice-nucleating particle concentration delayed ice formation. Our results suggest that collisions of supercooled water drops with ice particles may be a significant ice formation mechanism within shallow convective clouds where rime-splintering is not active.</p

    Plural relational green space values for whom, when, and where? - A social media approach

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    Unidad de excelencia María de Maeztu CEX2019-000940-MThe values people ascribe to their interactions with and within the environment are essential to inform justice and sustainability transformations. The development of many of these values unfolds through enjoying so-called cultural ecosystem services (CES) such as outdoor recreation, landscape aesthetics or environmental education. A growing body of literature is improving the assessment of the multiple ways that people value human and non-human relations arising when enjoying CES. Yet, the geo-temporal-demographic patterns of values distribution and the lessons that can be derived are to be consistently analysed within this relational framework. Building on a visual and textual content analysis of social media (SM) data geotagged in a peri-urban park of Barcelona, Spain, this research explores the potential of analysing the associated metadata (such as geotag, timestamp and social media users' demographics - i.e., performed gender and residency) in order to develop a better understanding of the linkages between people's values and the situated context of their construction. Our results show trends in relational CES values distribution along and between the analysed spatial, temporal, and demographic dimensions. In particular, despite there being a multiplicity of values revealed across the whole case-study area, to enjoy contemplative CES, such as spiritual or cognitive value, people need to move away from highly frequented areas and prefer specific times of the day, respectively evening or afternoon. Locals show a higher preference to visit the park on weekends compared to non-locals, while women-performing users show a significantly higher drop in their CES benefits uptake compared to men-performing users at night. In addition to providing novel and fine-grained information for transformative practices toward justice and sustainability, this study highlights the importance of complementing CES studies employing SM with metadata analysis to improve our understanding of the relationship between the real and the more-than-real
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