Gold nanoparticles/silver-bipyridine hybrid nanobelts with tuned peroxidase-like activity

Gold nanoparticles-decorated silver-bipyridine coordination polymers with intrinsic peroxidase-like activity are reported. Both morphology and mimetic enzyme activity can be tuned by rational manipulation of the nanohybrid composition. The nanomaterial was used for the electrochemical determination of H2O2 and glucose

Implementación de aplicaciones móviles como herramientas de enseñanza aprendizaje en Química Analítica

Depto. de Química AnalíticaFac. de Ciencias QuímicasFALSEsubmitte

Virtualización e impresión 3D de modelos anatómicos aplicados a la docencia en anatomia y cirugía veterinaria II

Este proyecto aplica las técnicas de virtualización de imágenes de piezas anatómicas normales y patológicas con fines de creación de modelos digitales y con impresión 3D para docencia en Anatomía y Cirugía Veterinarias

Amperometric aptasensor with sandwich-type architecture for troponin I based on carboxyethylsilanetriol-modified graphene oxide coated electrodes

A novel amperometric aptasensor for the specific detection of cardiac troponin I (cTnI) was constructed by using screen-printed carbon electrodes coated with a carboxyethylsilanetriol-modified graphene oxide derivative as transduction element. This novel carboxylic acid-enriched nanomaterial allows easy and high load immobilization of the capture aptamer molecules on the electrode surface. The biosensing interface was assembled by covalent attachment of an amino-functionalized DNA aptamer on the carboxylic acid-enriched electrode surface. The sensing approach relies on the specific recognition of cTnI by the aptamer and further assembly of a sandwich-type architecture with a novel aptamer-peroxidase conjugate as signaling element. The aptasensor was employed to detect the cardiac biomarker in the broad range from 1.0 pg/mL to 1.0 μg/mL with a detection limit of 0.6 pg/mL. This electroanalytical device also showed high specificity, reproducibility and stability, and was useful to quantify cTnI in reconstituted human serum samples.Spanish Ministry of Economy and CompetitivenessComunidad de MadridDepto. de Química AnalíticaFac. de Ciencias QuímicasTRUEpu

Amperometric aptasensor for carcinoembryonic antigen based on a reduced graphene oxide/gold nanoparticles modified electrode

We report the construction of a novel amperometric aptasensor for the highly specific detection of carcinoembryonic antigen (CEA). The sensing interface was assembled by electrodeposition of reduced graphene oxide (rGO) and gold nanoparticles (AuNPs) on a carbon screen-printed electrode (SPE) and further functionalization with a biotin and thiol-modified anti-CEA DNA hairpin aptamer. The sensing approach relies on the specific recognition of CEA by the folded aptamer, causing unfolding of the DNA hairpin structure and unmasking the biotin residues at the aptamer chain. Further incubation with a sptreptavidin-peroxidase conjugate allows the amperometric detection of the cancer biomarker. This aptasensor was able to detect CEA in the broad range from 20 pg mL−1 to 2 μg mL−1 (112 fM–11 μM) with a detection limit of 16 pg mL−1 (90 fM). The aptasensor also showed high reproducibility, specificity and stability, and was successfully validated in diluted human serum samples.Fil: Villalonga, Anabel. Universidad Complutense de Madrid; EspañaFil: Vegas, Borja. Universidad Complutense de Madrid; EspañaFil: Paniagua, Gonzalo. Universidad Complutense de Madrid; EspañaFil: Eguílaz Rubio, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Mayol, Beatriz. Universidad Complutense de Madrid; EspañaFil: Parrado, Concepción. Universidad Complutense de Madrid; EspañaFil: Rivas, Gustavo Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Díez, Paula. Universidad Complutense de Madrid; EspañaFil: Villalonga, Reynaldo. Universidad Complutense de Madrid; Españ