An Approach to operational control determination for the small or medium size organization

The purpose of this work was to design and assess an effective approach for a small or medium size organization to determine appropriate operational controls for its significant environmental aspects as part of developing and implementing an ISO 14001- type environmental management system. This new approach was determined to be necessary due to the generic nature and broad applicability of the ISO 14001 standard, the lack of appropriate methodology in the professional literature, and the lack of related knowledge and ability in most small or medium sized organizations. As controlling and influencing operations is a vital characteristic of an effective environmental management system, currently available federal and state guidance manuals for small or medium size environmental management systems were reviewed to determine the support that they provided in this area. Unfortunately, these guidance manuals lacked specific direction for identifying potential operational controls. Working with six organizations participating in the Monroe County Strategic Environmental Management Initiative, a new approach was developed and utilized to determine appropriate operational controls for each organization\u27s significant environmental aspects as part of its environmental management system development and implementation. This approach employed the use of: 1) An Operational Control Flowchart (developed for this thesis); 2) Decision Block Descriptions for the Operational Control Flowchart (developed in support of the Operational Control Flowchart); and 3) A cost-benefit analysis. Additionally, it was found that identifying and evaluating operational controls for one obvious environmental aspect in each organization before the organization\u27s environmental aspects were competitively evaluated for significance, provided a convincing example for skeptical participants and enhanced the EMS implementation process. The new approach began by first identifying potential operational controls for all identified significant aspects. Next, a practical cost-benefit analysis was performed for each of the potential operational controls before finalizing performance objectives and targets. As the potential operational controls were found to provide an economic as well as an environmental benefit, the MCSEMI participating organizations were eager to implement them and take advantage of the cost and environmental benefits

Charting a New Course: Professional Development Strategies for Improving Literacy Education Across the Curriculum

This paper reviews the effects of a program of professional development for literacy teachers in an urban, southeastern elementary school. During academic year 2002-2003, only 67% of fourth grade students met or exceeded state standards for achievement in reading as measured by the Criterion Referenced Competency Test (CRCT), and only 77% of fourth grade students met or exceeded state standards for achievement in English/language arts as measured by the CRCT (Georgia Department of Education, 2005)

Presence in Tibetan landscapes: spirited agency and ritual healing in Rebgong

This thesis intends to add to the field a sense of how deities pervade ordinary life in Tibetan cultural regions and what this means for those who live there. The study thus aims to develop understandings of the types of ritual healing which take place in this environment, one wherein landscapes are inhabited and experienced as embodiments of spirited agencies. In the thesis I suggest that, for my fieldwork regions of the Rebgong valley at least, ritual healing can best be understood a process by which beings are brought into right relations, both mutually and in connection with other beings, human and deity. I suggest here that all practices, whether ritual or medical, pertaining to health and well-being in Rebgong are predicated upon this type of epistemology; a cultural matrix of healing. This matrix is one in which healing is by definition is about humans and deities maintaining right relationship. I explore what this sensibility means for those who live in the Rebgong valleys primarily through ethnographic accounts of three particular ritual practices in Rebgong villages: the renewal of the labtsé tributes to the mountain gods, the Leru harvest ritual, and the performance of a tantric ritual cham dance. Forms of ritual healing I discuss in the thesis include circumambulation, medical and tantric practices, those of the trance or spirit mediums, dance and divination. I argue that all these rites and practices connected to health and well-being in a broad sense can be understood under one cultural matrix of healing in which spirited agency is focal. I argue that inherent to understanding this matrix is a focus on how deities, as embodied landscapes, appear within it, and how they are understood to exist and interact with human affairs, particularly those relating to health and well-being. In this regard, themes that I explore throughout the thesis are those of luck, purification, empowerment, embodiment and blessing. The study is intended, in a Bakhtinian sense, as a body of words which do not bring closure but rather seek to engage in a dialogical conversation that simultaneously responds to past scholarship and anticipates response

Using mentoring to improve the foundation placement in psychiatry: review of literature and a practical example

In the past few years, mentoring in clinical settings has attracted the attention of medical educators, clinicians, managers, and policy makers. Most of the Royal Colleges of medical and surgical specialities have some form of mentoring schemes and various regional divisions of Health Education England support mentoring and coaching in the workplace. Despite the importance of this topic and the great need to provide more support to doctors in recent times, there is a paucity of literature on examples of mentoring schemes in clinical settings and practicalities of setting up such schemes in hospitals. This paper describes the implementation of a mentoring scheme in a large mental health trust in the UK to support junior doctors and the issues involved in creating such scheme. We hope that this article will be useful to clinicians who would like to start similar schemes in their workplace

The changing role of Advanced Clinical Practitioners working with older people during the COVID- 19 pandemic: A qualitative research study

Background: COVID-19 was identified as a pandemic by the World Health Organisation (WHO) in December 2020. Advanced Clinical Practitioners (ACPs) in England working with older people with frailty, experienced their clinical role changing in response to the emergency health needs of this complex population group. In contrast to other countries, in England Advanced Clinical Practitioners are drawn from both nursing and allied health professions. Whilst much of the literature emphasises the importance of ensuring the sustainability of the Advanced Clinical Practitioners’ role, the pandemic threw further light on its potential and challenges. However, an initial review of the literature highlighted a lack of research of Advanced Clinical Practitioners’ capabilities working with uncertainty in disaster response situations. Aim: To capture the lived experience of how English Advanced Clinical Practitioners working with older people adapted their roles in response to the COVID-19 pandemic (October 2020–January 2021). Design, setting and participants: A qualitative research design was used. Following ethical approval, 23 Advanced Clinical Practitioner volunteer participants from across England with varied health professional backgrounds were recruited from Advanced Clinical Practitioners’ professional and social media networks on Twitter using a snowballing technique. Methods: Depending on preference or availability, 23 participants (nurses (18), physiotherapists (2), paramedics (2) and a pharmacist (1)) were interviewed singularly (n = 9) or as part of 3 focus groups (n = 14) using Zoom video communication. Audio recordings were transcribed and using qualitative data analysis software, NVivo 12 pro, coded for an essentialist thematic analysis of Advanced Clinical Practitioners’ responses using an inductive approach. 27 codes were identified and collated into five themes. For the purposes of this paper, four themes are discussed: experiencing different work, developing attributes, negotiating barriers and changing future provision. Findings: Advanced Clinical Practitioners successfully transferred their advanced practice skills into areas of clinical need during the pandemic. Their autonomous and generic, high level of expertise equipped them for management and leadership positions where speed of change, and the dissolution of traditional professional boundaries, were prioritised. Barriers to progress included a lack of knowledge of the Advanced Clinical Practitioner role and friction between Advanced Clinical Practitioners and physicians. Discussion and conclusion: The study demonstrated the successful adaption of the Advanced Clinical Practitioner role to enable more creative, personalised and sustainable solutions in the care of older people living with frailty during the pandemic. The potential of Advanced Clinical Practitioner development is in a juxtaposition to the threat of pandemic services being dismantled once the emergency nature of care has passed. Healthcare organisations have a vital part to play in considering the enablers and barriers of Advanced Clinical Practitioner capability-based practice when responding to uncertainty

Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components

The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions. Here, we report a pipeline for the rapid design, assembly, and validation of cell-free, paper-based sensors for the detection of the Zika virus RNA genome. By linking isothermal RNA amplification to toehold switch RNA sensors, we detect clinically relevant concentrations of Zika virus sequences and demonstrate specificity against closely related Dengue virus sequences. When coupled with a novel CRISPR/Cas9-based module, our sensors can discriminate between viral strains with single-base resolution. We successfully demonstrate a simple, field-ready sample-processing workflow and detect Zika virus from the plasma of a viremic macaque. Our freeze-dried biomolecular platform resolves important practical limitations to the deployment of molecular diagnostics in the field and demonstrates how synthetic biology can be used to develop diagnostic tools for confronting global health crises.Defense Threat Reduction Agency (DTRA) (HDTRA1-14-1-0006)United States. National Institutes of Health (NIH AI100190

Ratel: MPC-extensions for Smart Contracts

Enhancing privacy on smart contract-enabled blockchains has garnered much attention in recent research. Zero-knowledge proofs (ZKPs) is one of the most popular approaches, however, they fail to provide full expressiveness and fine-grained privacy. To illustrate this, we underscore an underexplored type of Miner Extractable Value (MEV), called Residual Bids Extractable Value (RBEV). Residual bids highlight the vulnerability where unfulfilled bids inadvertently reveal traders’ unmet demands and prospective trading strategies, thus exposing them to exploitation. ZKP-based approaches failed to ad- dress RBEV as they cannot provide post-execution privacy without some level of information disclosure. Other MEV mitigations like fair-ordering protocols also failed to address RBEV. We introduce Ratel, an innovative framework bridging a multi-party computation (MPC) prototyping framework (MP-SPDZ) and a smart contract language (Solidity), harmonizing the privacy with full expressiveness of MPC with Solidity ’s on-chain programmability. This synergy empowers developers to effortlessly craft privacy-preserving decentralized applications (DApps). We demonstrate Ratel’s efficacy through two distinguished decentralized finance (DeFi) applications: a decentralized exchange and a collateral auction, effectively mitigating the potential RBEV issue. Furthermore, Ratel is equipped with a lightweight crash-reset mechanism, enabling the seamless recovery of transiently benign faulty nodes. To prevent the crash-reset mechanism abused by malicious entities and ward off DoS attacks, we incorporate a cost-utility analysis anchored in the Bayesian approach. Our performance evaluation of the applications developed under the Ratel framework underscores their competency in managing real-world peak-time workloads

Aminopyrazine Inhibitors Binding to an Unusual Inactive Conformation of the Mitotic Kinase Nek2: SAR and Structural Characterization†

We report herein the first systematic exploration of inhibitors of the mitotic kinase Nek2. Starting from HTS hit aminopyrazine 2, compounds with improved activity were identified using structure-based design. Our structural biology investigations reveal two notable observations. First, 2 and related compounds bind to an unusual, inactive conformation of the kinase which to the best of our knowledge has not been reported for other types of kinase inhibitors. Second, a phenylalanine residue at the center of the ATP pocket strongly affects the ability of the inhibitor to bind to the protein. The implications of these observations are discussed, and the work described here defines key features for potent and selective Nek2 inhibition, which will aid the identification of more advanced inhibitors of Nek2

Conofolidine, a Natural Plant Alkaloid Causes Apoptosis and Senescence in Cancer Cells

Natural products contribute substantially to anticancer therapy; the plant kingdom provides an important source of molecules. Conofolidine is a novel Aspidosperma-Aspidosperma bisindole alkaloid isolated from the Malayan plant Tabernaemontana corymbosa. Herein, we report conofolidine’s anticancer activity together with that of three other bisindoles - conophylline, leucophyllidine and bipleiophylline against human-derived carcinoma cell lines. Remarkably, conofolidine was able to induce apoptosis (as observed in MDA-MB-468 breast cancer cells) or senescence (as detected in HT-29 colorectal carcinoma cells). Annexin V-FITC/PI, caspase activation and PARP cleavage confirmed the former while positive β-gal staining corroborated the latter. Evident cell cycle perturbations were observed comprising S-phase depletion, accompanied by downregulated CDK2, and cyclins (A2, D1) with p21 upregulation. Confocal imaging of HCT-116 cells revealed induction of aberrant mitotic phenotypes - multi-nucleation, membrane blebbing and DNA-fragmentation. The DNA integrity assessment of HCT-116 and MDA-MB-468 showed irreparable damage identified by increased fluorescent γ-H2AX during the G1 cell cycle phase. Furthermore, γ-H2AX foci were visually validated in HCT-116 and MDA-MB-468 cells using confocal microscopy. Conofolidine increased oxidative stress, preceding apoptosis- and senescence-induction in most carcinoma cell lines as seen by enhanced ROS levels accompanied by NQO1 expression. Collectively, we present conofolidine as a potential anticancer candidate capable of inducing heterogeneous modes of cancer cell death in vitro, encouraging further preclinical evaluation of this natural product