Kaplan-Meier analysis of survival in a cohort of IPF patients.

<p>(A) Carriers of the <i>TP53</i> rs12951053 C or rs12602273 G alleles had significantly worse 4-year survival rate (p = 0.006). (B) There was no significant difference in survival between carriers and non-carriers of the <i>CDKN1A</i> rs2395655 G allele (p = 0.2).</p

<i>TP53</i> genotype frequency in patients and controls.

<p>The values in parentheses are the number of individuals the frequency is based on.</p><p>There were no significant differences between patients and controls.</p

Linkage disequilibrium plot.

<p>Showing pairwise r² for SNPs in <i>TP53</i> (A) and <i>CDKN1A</i> (B).</p

<i>CDKN1A</i> mRNA expression in healthy controls.

<p><i>CDKN1A</i> mRNA levels were significantly different between rs733590 genotypes, p = 0.03 (TT+CT vs. CC). A similar trend was observed for rs2395655 genotypes (p = 0.06, AA+AG vs. GG).</p

Genotype and lung function decline.

<p>Number of IPF patients with non-rapid or rapid decline in %predicted vital capacity (>10% in one year) or %predicted diffusion capacity (>15% in one year).</p><p>*Fisher's exact test with carriers of p21 rs2395655G vs. non-carriers resulted in p = 0.04.</p

<i>CDKN1A</i> genotype and carriership frequency in patients and controls.

<p>The values in parentheses are the number of individuals. P values are based on the number of individuals with and without the specified genotype and are calculated using a Pearson's goodness-of-fit chi-square test. Odds ratio (OR) is shown with the 95% confidence interval in brackets.</p><p>*After correction for multiple testing rs733590 and rs2395655 remained significant.</p

Data Collection Codes

Collection details pertaining to the Albinaria samples, some containing Drilus laevae or adult, that were used in this stud

Appendix_PLOS ONE (herzien)- Voucher numbers - RMNH

This file contains all information relating to the Drilus specimens (adults, larvae, and exuviae) obtained from snail shells in the course of this study. Voucher numbers refer to the official collections in which the specimens were deposited

Greek <i>Albinaria</i> snails and their <i>Drilus</i> predators.

<p>A, <i>Albinaria hippolyti</i> from Crete (photo: V. Wiese). B and C, the clausilium, shown in the shell aperture after removal of the left lateral shell wall (B shows a less-obstructing, N-type clausilium, C shows a more obstructing, G-type clausilium). D and E, a male and a female, respectively, of a yet undescribed <i>Drilus</i> species from Crete (scale: 2 mm). F, a full-grown larva of <i>Drilus</i> “L” from the Peloponnese (same scale as D and E). G, an estivating <i>A. discolor</i> from the Peloponnese, with a <i>Drilus</i> exit bore hole. H, a <i>Drilus</i> “L” exiting from its prey, an <i>A. menelaus</i> from the Peloponnese.</p

A, five <i>Drilus</i> species from the Peloponnese have different specificities for <i>Albinaria</i> as prey, and concomitant propensities to bore holes in the shell (calculated as the number of bore holes divided by the total number of prey).

<p>B, difference (<i>P</i><0.05, Fisher's exact test) in prey specificity between <i>Drilus</i> “D” (more <i>Albinaria</i>-specific) and “E” (less <i>Albinaria</i> specific) in two localities where both species occur syntopically (these data are not included in fig. 3A). C, differences in <i>Drilus</i> predation rate (dark portion of the bar) between solitarily (“sol”) and group-wise (“agg”) estivating snails of <i>A. caerulea</i> in four 5 m² plots in the islands of Paros, Naxos, and Thira (voucher numbers RMNH.MOL.84354-84363, RMNH.MOL.85192, and RMNH.MOL.85193). An aggregate was defined as a cluster of >20 snails, with distances of <2 cm separating them. A snail was considered solitary if it was >20 cm distance from a conspecific (significance tested with Chi-square test). D, positions of entrance holes in shells of species with an N-type clausilium compared with those in shells of species with a G-type clausilium, shown for the entire eastern Peloponnese as well as for the site Monemvasia, where both clausilium types occur microsympatrically. (<i>P</i>-values are derived from Fisher's exact test.)</p