ELECTROPHYSIOLOGICAL PROPERTIES OF HUMAN OCULAR EXTRAMISSION

The author presents evidence supporting the hypothesis that human ocular extramission, the emission of brain waves through the eye, can be detected using qEEG equipment or two-channel neurofeedback equipment. A high-impedance electrode housed inside electromagnetically insulated goggles was employed that makes no physical contact with the body and is about two centimeters in front of the pupil. Readings were taken with two-channel biofeedback equipment and with a qEEG The waveform of ocular extramission is physiologically active compared to the reading from a control electrode suspended in space in front of the goggles: it is similar to the waveform of frontal leads in overall structure and in the appearance of eye blink and muscle artifact in the tracing, combined with an absence of heart artifact. The waveform for a control electrode showed only consistent high-frequency, low amplitude background and heart beat artifact. It may be possible to study a variety of disease states by recording ocular extramission, using a high-impedance noncontact electrode

ELECTROPHYSIOLOGICAL PROPERTIES OF HUMAN OCULAR EXTRAMISSION

The author presents evidence supporting the hypothesis that human ocular extramission, the emission of brain waves through the eye, can be detected using qEEG equipment or two-channel neurofeedback equipment. A high-impedance electrode housed inside electromagnetically insulated goggles was employed that makes no physical contact with the body and is about two centimeters in front of the pupil. Readings were taken with two-channel biofeedback equipment and with a qEEG The waveform of ocular extramission is physiologically active compared to the reading from a control electrode suspended in space in front of the goggles: it is similar to the waveform of frontal leads in overall structure and in the appearance of eye blink and muscle artifact in the tracing, combined with an absence of heart artifact. The waveform for a control electrode showed only consistent high-frequency, low amplitude background and heart beat artifact. It may be possible to study a variety of disease states by recording ocular extramission, using a high-impedance noncontact electrode

SIMULTANEOUS VARIATION IN THE HEART AND BRAIN ELECTRICAL FIELDS

The author measured a participant’s heart and brain electrical fields simultaneously using EEG electrodes and neurofeedback software while her psychophysiological state was being varied. In the first session, the participant was in an awake-alert state in bright light; in the second she was in a drowsy state in dim light; in both conditions recordings were made with eyes open and eyes closed. Significant differences in EEG amplitude were found in both the brain and heart electrical fields comparing the psychophysiological states of eyes open/eyes closed and alert/drowsy. It is possible to study the participation of peripheral nerve centers such as the cardiac plexus in psychophysiological state change and information processing

Instability of fixed, low-thrust drag compensation

FORCED drag compensation using continuous low-thrustpropulsion has been considered for satellites in low Earth orbit. This simple, but nonoptimal, scheme merely requires that the thrust vector is directed opposite to the drag vector and that the magnitude of the two are equal. In principle, the drag force acting on the spacecraft could be determined onboard using accurate accelerometers. However, for small, low-cost spacecraft such sensors may beunavailable. An alternative strategy would be to Ž x the thrust magnitude equal to the expected air drag that would be experienced by the spacecraft. The thrust levelwould be periodically updated based on ground-based orbit determination. In this Engineering Note, it is shown that such a forced circular orbit with a Ž fixed thrust levelis exponentially unstable for all physically reasonable atmosphere models

Impact of Environmental Factors on Bacteriocin Promoter Activity in Gut-Derived Lactobacillus salivarius

peer-reviewedBacteriocin production is regarded as a desirable probiotic trait that aids in colonization and persistence in the gastrointestinal tract (GIT). Strains of Lactobacillus salivarius, a species associated with the GIT, are regarded as promising probiotic candidates and have a number of associated bacteriocins documented to date. These include multiple class IIb bacteriocins (salivaricin T, salivaricin P, and ABP-118) and the class IId bacteriocin bactofencin A, which show activity against medically important pathogens. However, the production of a bacteriocin in laboratory media does not ensure production under stressful environmental conditions, such as those encountered within the GIT. To allow this issue to be addressed, the promoter regions located upstream of the structural genes encoding the L. salivarius bacteriocins mentioned above were fused to a number of reporter proteins (green fluorescent protein [GFP], red fluorescent protein [RFP], and luciferase [Lux]). Of these, only transcriptional fusions to GFP generated signals of sufficient strength to enable the study of promoter activity in L. salivarius. While analysis of the class IIb bacteriocin promoter regions indicated relatively weak GFP expression, assessment of the promoter of the antistaphylococcal bacteriocin bactofencin A revealed a strong promoter that is most active in the absence of the antimicrobial peptide and is positively induced in the presence of mild environmental stresses, including simulated gastric fluid. Taken together, these data provide information on factors that influence bacteriocin production, which will assist in the development of strategies to optimize in vivo and in vitro production of these antimicrobials.This work was funded by a SFI PI award “Obesibiotics” (11/PI/1137) to PD

ΦCrAss001 represents the most abundant bacteriophage family in the human gut and infects Bacteroides intestinalis

peer-reviewedCrAssphages are an extensive and ubiquitous family of tailed bacteriophages, predicted to infect bacteria of the order Bacteroidales. Despite being found in ~50% of individuals and representing up to 90% of human gut viromes, members of this viral family have never been isolated in culture and remain understudied. Here, we report the isolation of a CrAssphage (ΦCrAss001) from human faecal material. This bacteriophage infects the human gut symbiont Bacteroides intestinalis, confirming previous in silico predictions of the likely host. DNA sequencing demonstrates that the bacteriophage genome is circular, 102 kb in size, and has unusual structural traits. In addition, electron microscopy confirms that ΦcrAss001 has a podovirus-like morphology. Despite the absence of obvious lysogeny genes, ΦcrAss001 replicates in a way that does not disrupt proliferation of the host bacterium, and is able to maintain itself in continuous host culture during several weeks

Convergent validity of the new form of the DES

p. 101-103The line and circle farms of the Dissociative Experiences Scale (DES I and DES II) were administered to 65 subjects in the general population, 87 subjects with a clinical diagnosis of dissociative identity disorder, and 26 subjects with a diagnosis of chemical dependency. In all three samples the DES II showed excellent validity when compared to the original line form of the DES

Expression of KOC, S100P, mesothelin and MUC1 in pancreatico-biliary adenocarcinomas: development and utility of a potential diagnostic immunohistochemistry panel

<b>Background</b> Pancreatico-biliary adenocarcinomas (PBA) have a poor prognosis. Diagnosis is usually achieved by imaging and/or endoscopy with confirmatory cytology. Cytological interpretation can be difficult especially in the setting of chronic pancreatitis/cholangitis. Immunohistochemistry (IHC) biomarkers could act as an adjunct to cytology to improve the diagnosis. Thus, we performed a meta-analysis and selected KOC, S100P, mesothelin and MUC1 for further validation in PBA resection specimens.<p></p> <b>Methods</b> Tissue microarrays containing tumour and normal cores in a ratio of 3:2, from 99 surgically resected PBA patients, were used for IHC. IHC was performed on an automated platform using antibodies against KOC, S100P, mesothelin and MUC1. Tissue cores were scored for staining intensity and proportion of tissue stained using a Histoscore method (range, 0–300). Sensitivity and specificity for individual biomarkers, as well as biomarker panels, were determined with different cut-offs for positivity and compared by summary receiver operating characteristic (ROC) curve.<p></p> <b>Results</b> The expression of all four biomarkers was high in PBA versus normal ducts, with a mean Histoscore of 150 vs. 0.4 for KOC, 165 vs. 0.3 for S100P, 115 vs. 0.5 for mesothelin and 200 vs. 14 for MUC1 (p < .0001 for all comparisons). Five cut-offs were carefully chosen for sensitivity/specificity analysis. Four of these cut-offs, namely 5%, 10% or 20% positive cells and Histoscore 20 were identified using ROC curve analysis and the fifth cut-off was moderate-strong staining intensity. Using 20% positive cells as a cut-off achieved higher sensitivity/specificity values: KOC 84%/100%; S100P 83%/100%; mesothelin 88%/92%; and MUC1 89%/63%. Analysis of a panel of KOC, S100P and mesothelin achieved 100% sensitivity and 99% specificity if at least 2 biomarkers were positive for 10% cut-off; and 100% sensitivity and specificity for 20% cut-off.<p></p> <b>Conclusion</b> A biomarker panel of KOC, S100P and mesothelin with at least 2 biomarkers positive was found to be an optimum panel with both 10% and 20% cut-offs in resection specimens from patients with PBA.<p></p&gt

Listeriolysin S, a Novel Peptide Haemolysin Associated with a Subset of Lineage I Listeria monocytogenes

peer-reviewedStreptolysin S (SLS) is a bacteriocin-like haemolytic and cytotoxic virulence factor that plays a key role in the virulence of Group A Streptococcus (GAS), the causative agent of pharyngitis, impetigo, necrotizing fasciitis and streptococcal toxic shock syndrome. Although it has long been thought that SLS and related peptides are produced by GAS and related streptococci only, there is evidence to suggest that a number of the most notorious Gram-positive pathogenic bacteria, including Listeria monocytogenes, Clostridium botulinum and Staphylococcus aureus, produce related peptides. The distribution of the L. monocytogenes cluster is particularly noteworthy in that it is found exclusively among a subset of lineage I strains; i.e., those responsible for the majority of outbreaks of listeriosis. Expression of these genes results in the production of a haemolytic and cytotoxic factor, designated Listeriolysin S, which contributes to virulence of the pathogen as assessed by murine- and human polymorphonuclear neutrophil–based studies. Thus, in the process of establishing the existence of an extended family of SLS-like modified virulence peptides (MVPs), the genetic basis for the enhanced virulence of a proportion of lineage I L. monocytogenes may have been revealed.Work is funded by the Irish Government under the National Development Plan, through a Science Foundation Ireland Investigator award to CH, PR and PC (06/IN.1/B98)