Effect of laser on the bone defects healing in rats: An experimental study

Introduction. The stimulating effect of low-power laser on the process of wound healing is characterized by proliferation of fibroblasts, collagen production, and enhanced enzyme activity. The aim of this study was to evaluate the possible enhancing effect of low-power laser on the rate of the healing artificially created osseous defects in rats. Material and methods. Ten albino Wister rats were involved in this experimental study. Round defects (3 mm wide 2 mm deep) were made in each rat on both femurs. The right side was experimental while the left side was control. Osseous defects on the experimental side were daily treated with Galium, Aluminium, Arsenid (GaAlAs) low-power laser (Medicolaser 637, Technoline, Belgrade, Serbia), at the energy output of 4 J/cm2, with constant power density of 50 mW, and a wave length 637 nm (visible red light) per defect during seven days. Defects on the control side healed spontaneously. The effects of laser were evaluated two and three weeks postoperatively. Results. Histological analysis showed the powerful osteoblastic activity on the bone defects of the experimental side two weeks after surgery. On the control side, a new bone formation was noticed at the periphery of the bone defects but fibroblastic tissue with no signs of new bone was presented in the central areas. Three weeks after surgery, on the experimental side, bone defects were completely filled with spongious, lamellar bone while non-treated bone defects were characterized by mature lamellar bone at the peripheral areas and immature bone at the central areas. Conclusion. The results showed that the use of low-power laser could have a significant influence on the speed of curing bone defects in rats.Uvod. Laseri male snage utiču na proces zarastanja rana, koji odlikuju proliferacija fibroblasta, brže stvaranje kolagena i pojačana aktivnost enzima. Cilj ovog rada je bio da se proceni efekat lasera male snage na zarastanje veštački izazvanih oštećenja kosti laboratorijskih pacova. Materijali i metode rada. U istraživanju je korišćeno 10 albino vister pacova. Kod svakog od njih su na obe butne kosti načinjena okrugla oštećenja (3 mm široka i 2 mm duboka), s tim da je desna bila eksperimentalna strana, a leva kontrolna. Eksperimentalne strane su svakodnevno tretirane galijum-aluminijum-arsenid (GaAIAs) mekim laserom (Medicolaser 637, Technoline, Belgrade, Serbia) sa izlaznom snagom od 50 mW, koristeći 4J/cm2 po oštećenju tokom sedam dana. Kontrolne strane su ostavljene da spontano zarastu. Efekti ozračenosti laserom su procenjivani dve i tri nedelje posle operacije. Rezultati. Dve nedelje nakon operacije histološkom analizom oštećenja kosti eksperimentalne strane uočena je snažna aktivnost osteoblasta. U istovremena kontrolnoj strani zapaženi su novoformirani koštani izdanci na periferiji oštećenja kosti, odnosno fibroblastično tkivo bez znakova stvaranja nove kosti u centralnom delu oštećenja. Tri nedelje nakon operacije eksperimentalna oštećenja kosti su potpuno bila ispunjena sunđerastom lamelarnom kosti, dok su netretirana oštećenja kosti odlikovale zrela lamelama kost na perifernim delovima i nezrela kost u centralnim delovima. Zaključak. Dobijeni nalazi pokazuju da korišćenje lasera male snage može značajno uticati na brzinu zarastanja oštećene kosti kod laboratorijskih životinja

The Inflammatory Radicular Cysts Have Higher Concentration of TNF-α in Comparison to Odontogenic Keratocysts (Odontogenic Tumour)

TNF-α is a pleiotropic cytokine that is considered as a primary modifier of inflammatory and immune reaction in response to various inflammatory diseases and tumour. We investigated levels of TNF-α in 43 radicular cysts and 15 odontogenic keratocysts, obtained from patients undergoing surgery, under local anaesthesia, and after aspiration of cystic fluid from non-ruptured cysts. TNF-α is elevated in both cysts’ fluid, but higher values were found in radicular cysts in comparison to keratocysts. The significantly higher concentration of TNF-α was associated with smaller radicular cysts, higher protein concentration, higher presence of inflammatory cells in peri cystic tissues, and the degree of vascularisation and cysts wall thickness (Mann-Whitney U-test, p<0.05). No correlation was found based on these parameters in odontogenic keratocyst, but all cysts have detectable concentrations of TNF-α. We here for the first time present that a difference in the concentration of TNF-α exists between these two cystic types

An open prospective single cohort multicenter study evaluating the novel, tapered, conical connection implants supporting single crowns in the anterior and premolar maxilla : interim 1-year results

Objectives The aim of this multicenter prospective clinical study was to evaluate anodized tapered implants with a conical connection and integrated platform shifting placed in the anterior and premolar maxilla. Materials and methods The study enrolled patients requiring single-tooth restorations in healed sites of maxillary anterior and premolar teeth. All implants were immediately temporized. Clinical and radiographic evaluations were conducted at implant insertion, 6 months, and 1 year. Outcome measures included bone remodeling, cumulative survival rate (CSR), success rate, soft-tissue health and esthetics, and patient satisfaction. Bone remodeling and pink esthetic score were analyzed using Wilcoxon signed-rank tests. CSR was calculated using life table analysis. Other soft-tissue outcomes were analyzed using sign tests. Results Out of 97 enrolled patients (102 implants), 87 patients (91 implants) completed the 1-year visit. Marginal bone remodeling was 0.85 1.36 mm. After the expected initial bone loss, a mean bone gain of 0.11 1.05 mm was observed between 6 months and 1 year. The CSR was 99.0%, and the cumulative success rate was 97.0%. Partial or full papilla was observed at 30.8% of sites at baseline, 87.2% at 6 months, and 90.5% at 1 year. Soft-tissue response, esthetics, and patient satisfaction all improved during the study period. Conclusions Bone gain was observed following the expected initial bone loss, and soft-tissue outcomes improved suggesting favorable tissue response using anodized tapered conical connection implants. Clinical relevance Rapid stabilization of bone remodeling and robust papilla regeneration indicate favorable tissue healing promoted by the conical connection, platform-shift design. Trial registration clinicaltrials.gov NCT02175550(VLID)354529