Combined coagulation and inflammation markers as predictors of venous thrombo-embolism and death in COVID-19

BackgroundThe COVID-19 pandemic related to SARS-CoV-2 virus was responsible for global pandemic. The severe form of the disease was linked to excessive activation of immune pathways together with a systemic cytokine storm response and thrombotic venous or arterial complications. Factors predicting severe outcomes including venous and/or pulmonary thrombosis (VT) and death were identified, but the prognostic role of their combination was not addressed extensively.ObjectivesWe investigated the role of prognostic factors from the coagulation or inflammatory pathways to better understand the outcome of the disease.MethodsFor this, we prospectively studied 167 SARS-CoV-2-positive patients from admission in intensive care units (ICU) or emergency departments from four academic hospitals over a 14-month period. Besides standard biology, we assessed serum concentrations of inflammatory markers, coagulation factors and peripheral blood cells immunophenotyping.ResultsThirty-nine patients (23.3%) developed VT and 30 patients (18%) died. By univariate analysis, C-reactive protein (CRP) level > 150 mg/L, interleukin-6 (IL-6) ≥ 20 pg/mL, D-dimers > 1,500 μg/L, ADAMTS13 activity ≤ 50%, VonConclusionA combination of coagulation and inflammatory markers can refine the prognostication of severe outcome in COVID-19, and could be useful for the initial evaluation of other types of viral infection

Translational regulation by RACK1 in astrocytes represses KIR4.1 expression and regulates neuronal activity

Summary The regulation of translation in astrocytes, the main glial cells in the brain, remains poorly characterized. We developed a high-throughput proteomic screen for polysome-associated proteins in astrocytes and focused on the ribosomal protein receptor of activated protein C kinase 1 (RACK1), a critical factor in translational regulation. In astrocyte somata and perisynaptic astrocytic processes (PAPs), RACK1 preferentially bound to a number of mRNAs, including Kcnj10 , encoding the inward rectifying potassium (K + ) channel KIR4.1, a critical astrocytic regulator of neurotransmission. By developing an astrocyte-specific, conditional RACK1 knock-out mouse model, we showed that RACK1 repressed the production of KIR4.1 in hippocampal astrocytes and PAPs. Reporter-based assays revealed that RACK1 controlled Kcnj10 translation through the transcript’s 5’ untranslated region. Upregulation of KIR4.1 in the absence of RACK1 modified the astrocyte territory volume and neuronal activity attenuatin burst frequency and duration in the hippocampus. Hence, astrocytic RACK1 represses KIR4.1 translation and influences neuronal activity

The “FAME” Interactive Space

International audienceThis paper describes the “FAME” multi-modal demonstrator, which integrates multiple communication modes – vision, speech and object manipulation – by combining the physical and virtual worlds to provide support for multi-cultural or multi-lingual communication and problem solving. The major challenges are automatic perception of human actions and understanding of dialogs between people from different cultural or linguistic backgrounds. The system acts as an information butler, which demonstrates context awareness using computer vision, speech and dialog modeling. The integrated computerenhanced human-to-human communication has been publicly demonstrated at the FORUM2004 in Barcelona and at IST2004 in The Hague. Specifically, the “Interactive Space” described features an “Augmented Table” for multi-cultural interaction, which allows several users at the same time to perform multi-modal, cross-lingual document retrieval of audio-visual documents previously recorded by an “Intelligent Cameraman” during a week-long seminar