Spectroscopic investigations of iron(II) and iron(III) oxalates

In an attempt to contribute to a better characterization of FeII and FeIII oxalate complexes, an investigation of their vibrational (infrared and Raman) and 57Fe-Mössbauer spectra was performed. It is shown that the two polymorphs, α and β, of FeC2O4.2H2O cannot be accurately differentiated with any of these spectroscopic methods, demonstrating the high structural similarity of these two crystalline forms. Partially deuterated samples of β-FeC2O4·2H2O were also investigated to improve the vibrational-spectroscopic analysis. In the case of Fe2(C2O4)3·4H2O, a structural model, derived from results of combined vibrational and Mössbauer data, could be proposed. The 298 K Mössbauer spectrum for this complex is discussed in detail for the first time.Facultad de Ciencias ExactasCentro de Química Inorgánic

Anti-metastatic action of an N4-aryl substituted thiosemicarbazone on advanced triple negative breast cancer

Purpose: Advanced triple negative breast cancer (ATNBC) is defined by a lack of expression of hormones receptors as well as HER2/neu and its high probability of visceral metastasis. This pathology is associated with a poor prognosis. Previously, we found that T2, an N4 -arylsubstituted thiosemicarbazone (N4 -TSC), had cytotoxic effect on human breast cancer cells lines. Hence, in this study, we investigated the anti-metastasic action of T2 on ATNBC. Methods: In order to deepen T2 action mode on ATNBC, we first confirmed T2 cytotoxicity on a panel of TNBC cells and then continued studying T2 effects in vitro an in vivo on the syngeneic 4T1 mouse model. Results: We found that T2 had a cytotoxic effect comparable to chemotherapeutics used in present treatment schemes for ATNBC. T2 treatment not only induced apoptosis, but it also down-modulated 4T1 invasive and metastatic-associated capacities, such as clonogenicity, migration and metallo-proteases activity. Moreover, this agent reduced the number of 4T1 cancer stem cells. Finally, T2 treatment induced a more differentiated cell phenotype and the overexpression of the metastasis suppressor gene NDRG-1. In vivo assays showed that T2 reduced tumor burden, down modulated local tumor invasion and significantly reduced the number of lung metastases in the 4T1 advanced TNBC murine model, while the compound did not exhibit intolerable toxicity. Conclusion: This study provided evidence that T2 not only exerted an anti-tumor activity but it also showed antiinvasive and anti-metastatic actions on ATNBC in vivo and in vitro, suggesting that T2 could be considered as a promising therapy that deserves further analysis.Fil: Sólimo, Aldana María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; ArgentinaFil: Soraires Santacruz, Maria Cristina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Química Medicinal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Vanzulli, Silvia. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; ArgentinaFil: Coggiola, Osvaldo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; ArgentinaFil: Bal, Elisa Dora. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Finkielsztein, Liliana Mónica. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Farmacología. Cátedra de Química Medicinal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Metabolismo del Fármaco; ArgentinaFil: Callero, Mariana Alejandra. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología "Ángel H. Roffo"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Spectroscopic investigations of iron(II) and iron(III) oxalates

In an attempt to contribute to a better characterization of FeII and FeIII oxalate complexes, an investigation of their vibrational (infrared and Raman) and 57Fe-Mössbauer spectra was performed. It is shown that the two polymorphs, α and β, of FeC2O4.2H2O cannot be accurately differentiated with any of these spectroscopic methods, demonstrating the high structural similarity of these two crystalline forms. Partially deuterated samples of β-FeC2O4·2H2O were also investigated to improve the vibrational-spectroscopic analysis. In the case of Fe2(C2O4)3·4H2O, a structural model, derived from results of combined vibrational and Mössbauer data, could be proposed. The 298 K Mössbauer spectrum for this complex is discussed in detail for the first time.Facultad de Ciencias ExactasCentro de Química Inorgánic

A compromising position for a variant: A new allele for D1S1656 that invades its neighbors and can lead to missinterpretations

Short tandem repeats (STRs) are the markers of choice for purposes of human forensic identification because of their considerable degree of polymorphism. This variability may occasionally become a challenge for the analyst when a new variant invades the allele distribution range of the neighboring locus. We present here a novel variant at locus D1S1656 showing a molecular length of 211.32 bp corresponding to 21.3 repeating units. This variant is superimposed on the D12S391 locus when the Global Filer™ (Thermo Fisher Scientific Inc., USA) is used, invading the shortest allele range and being assigned as Off-ladder (OL). In contrast, typing with PowerPlex® Fusion (Promega Corp., Madison, USA) overlaps the D2S441 locus, generating an allele that is recognized as 8. In both cases the invasion was observed as a tri-allele pattern. The results were confirmed by DNA re-extraction and typing with the two independent commercial megaplexes described above. The variant was detected during analysis of a reference sample throughout a paternity exclusion case. This is the first time this variant has been described and reported to the NIST STR Base. Since D1S1656 is included in widely-used multiplex kits from several vendors - Biotype (Dresden, Germany) Qiagen (Venlo, The Netherlands), Promega (Madison, USA) and Thermo Fisher (Foster City, CA, USA) - it would then be recommendable that other forensic labs be aware of this new micro-variant in dealing with similar interpretation challenges and that the kit producers take this fact into account in designing new multiplex kits.Fil: Marcucci, Valeria Cecilia. Universidad Nacional de la Patagonia Austral; ArgentinaFil: Cano, H.. Laboratorio Regional de Investigación Forense; ArgentinaFil: Coggiola, Liliana. Laboratorio Regional de Investigación Forense; ArgentinaFil: Mautner, M. E.. Biodynamics Sa; ArgentinaFil: Sala, Adriana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; ArgentinaFil: Corach, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Servicio de Huellas Digitales Genéticas; Argentin