2,381 research outputs found
SpMyb functions as an intramodular repressor to regulate spatial expression of CyIIIa in sea urchin embryos
The CyIIIa actin gene of Strongylocentrotus purpuratus is
transcribed exclusively in the embryonic aboral ectoderm,
under the control of 2.3 kb cis-regulatory domain that
contains a proximal module that controls expression in
early embryogenesis, and a middle module that controls
expression in later embryogenesis. Previous studies demonstrated that the SpRunt-1 target site within the middle
module is required for the sharp increase in CyIIIa transcription which accompanies differentiation of the aboral ectoderm, and that a negative regulatory region near the SpRunt-1 target site is required to prevent ectopic transcription in the oral ectoderm and skeletogenic mesenchyme. This negative regulatory region contains a
consensus binding site for the myb family of transcription
factors. In vitro DNA-binding experiments reveal that a
protein in blastula-stage nuclei interacts specifically with
the myb target site. Gene transfer experiments utilizing
CyIIIa reporter constructs containing oligonucleotide substitutions indicate that this site is both necessary and sufficient to prevent ectopic expression of CyIIIa. Synthetic oligonucleotides containing the myb target site were used to purify a protein from sea urchin embryo nuclear extracts by affinity chromatography. This protein is immunoprecipitated by antibodies specific to the evolutionarily conserved myb domain, and amino acid sequences obtained from the purified protein were found to be identical to sequences within the myb domain. Sequence information was used to obtain cDNA clones of SpMyb, the S. purpuratus member of the myb family of transcription factors. Through interactions within the middle module, SpMyb functions to repress activation of CyIIIa in the oral
ectoderm and skeletogenic mesenchyme
On local invariants of pure three-qubit states
We study invariants of three-qubit states under local unitary
transformations, i.e. functions on the space of entanglement types, which is
known to have dimension 6. We show that there is no set of six independent
polynomial invariants of degree less than or equal to 6, and find such a set
with maximum degree 8. We describe an intrinsic definition of a canonical state
on each orbit, and discuss the (non-polynomial) invariants associated with it.Comment: LateX, 13 pages. Minor typoes corrected. Published versio
Local symmetry properties of pure 3-qubit states
Entanglement types of pure states of 3 qubits are classified by means of
their stabilisers in the group of local unitary operations. It is shown that
the stabiliser is generically discrete, and that a larger stabiliser indicates
a stationary value for some local invariant. We describe all the exceptional
states with enlarged stabilisers.Comment: 32 pages, 5 encapsulated PostScript files for 3 figures. Published
version, with minor correction
Working paper series: A fifteen year review
In 1974, The Academy of Accounting. Historians established the Working Paper Series to provide Academy members a means of exposing historical research to a wider audience, exchanging of ideas, and providing feedback from other qualified persons interested in research
New Algorithms for Position Heaps
We present several results about position heaps, a relatively new alternative
to suffix trees and suffix arrays. First, we show that, if we limit the maximum
length of patterns to be sought, then we can also limit the height of the heap
and reduce the worst-case cost of insertions and deletions. Second, we show how
to build a position heap in linear time independent of the size of the
alphabet. Third, we show how to augment a position heap such that it supports
access to the corresponding suffix array, and vice versa. Fourth, we introduce
a variant of a position heap that can be simulated efficiently by a compressed
suffix array with a linear number of extra bits
Men’s Success in Higher Education: Model Building
This research addressed degree completion among adult male students. Findings illustrated that persistence depended on grit and a campus community where they could contribute and receive support to graduate
Molecular dynamics simulations on the Tre1 G protein-coupled receptor: Exploring the role of the arginine of the NRY motif in Tre1 structure
Abstract Background: The arginine of the D/E/NRY motif in Rhodopsin family G protein-coupled receptors (GPCRs) is conserved in 96% of these proteins. In some GPCRs, this arginine in transmembrane 3 can form a salt bridge with an aspartic acid or glutamic acid in transmembrane 6. The Drosophila melanogaster GPCR Trapped in endoderm-1 (Tre1) is required for normal primordial germ cell migration. In a mutant form of the protein, Tre1sctt, eight amino acids RYILIACH are missing, resulting in a severe disruption of primordial germ cell development. The impact of the loss of these amino acids on Tre1 structure is unknown. Since the missing amino acids in Tre1sctt include the arginine that is part of the D/E/NRY motif in Tre1, molecular dynamics simulations were performed to explore the hypothesis that these amino acids are involved in salt bridge formation and help maintain Tre1 structure. Results: Structural predictions of wild type Tre1 (Tre1+) and Tre1sctt were subjected to over 250 ns of molecular dynamics simulations. The ability of the model systems to form a salt bridge between the arginine of the D/E/NRY motif and an aspartic acid residue in transmembrane 6 was analyzed. The results indicate that a stable salt bridge can form in the Tre1+ systems and a weak salt bridge or no salt bridge, using an alternative arginine, is likely in the Tre1sctt systems. Conclusions: The weak salt bridge or lack of a salt bridge in the Tre1sctt systems could be one possible explanation for the disrupted function of Tre1sctt in primordial germ cell migration. These results provide a framework for studying the importance of the arginine of the D/E/NRY motif in the structure and function of other GPCRs that are involved in cell migration, such as CXCR4 in the mouse, zebrafish, and chicken
Full separability criterion for tripartite quantum systems
In this paper, an intuitive approach is employed to generalize the full
separability criterion of tripartite quantum states of qubits to the
higher-dimensional systems (Phys. Rev. A \textbf{72}, 022333 (2005)). A
distinct characteristic of the present generalization is that less restrictive
conditions are needed to characterize the properties of full separability.
Furthermore, the formulation for pure states can be conveniently extended to
the case of mixed states by utilizing the kronecker product approximate
technique. As applications, we give the analytic approximation of the criterion
for weakly mixed tripartite quantum states and investigate the full
separability of some weakly mixed states.Comment: 5 pages. To be published in Europ. J.
Unified Solution of the Expected Maximum of a Random Walk and the Discrete Flux to a Spherical Trap
Two random-walk related problems which have been studied independently in the
past, the expected maximum of a random walker in one dimension and the flux to
a spherical trap of particles undergoing discrete jumps in three dimensions,
are shown to be closely related to each other and are studied using a unified
approach as a solution to a Wiener-Hopf problem. For the flux problem, this
work shows that a constant c = 0.29795219 which appeared in the context of the
boundary extrapolation length, and was previously found only numerically, can
be derived explicitly. The same constant enters in higher-order corrections to
the expected-maximum asymptotics. As a byproduct, we also prove a new universal
result in the context of the flux problem which is an analogue of the Sparre
Andersen theorem proved in the context of the random walker's maximum.Comment: Two figs. Accepted for publication, Journal of Statistical Physic
Clean heating and heating poverty: A perspective based on cost-benefit analysis
To improve the air quality in winter, clean heating policy was implemented in “2 + 26” cities of China in 2016, which mainly included replacing coal with gas or electricity. Tremendous financial subsidies have been provided by city and central governments. This new heating mode changed the heating fee-cost to residents. This paper estimates the economic costs to both governments and residents, and evaluates the environmental and public health benefits by combining a difference-in-differences model with an exposure-response function. Results show that the total costs of clean heating were up to 43.1 billion yuan. Governments and residents account for 44% and 56% of the total costs, respectively. In terms of benefits, the clean heating project is effective for air pollution control and brings health economic benefits of about 109.85 billion yuan (95% CI: 22.40–159.83). The clean heating policy was identified as a net-positive benefit program with environmental and public health improvements. However, the inequality in subsidies from different cities governments increases the heating burden on low-income households and leads to heating poverty for households in the less developed regions. We provide suggestions for implementation in future clean heating campaigns and in subsidy mechanism design in China and for other developing countries
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