Izolarea colagenului din complexul ombilico-placentar pentru utilizarea în ingineria tisulară

Background. Collagen-based bone substitutes are widely used in bone tissue regeneration in orthopedic, traumatic and oromaxillofacial surgery. The predominant sources of collagen extraction are from animals, but they can transmit zoonoses from animal to human. Thus, collagen from the umbilicalplacental complex is a particular interest in the use as a graft for bone regeneration. Objective of the study. Obtaining collagen from the umbilical-placental complex for use in tissue engineering. Material and Methods. Collagen was isolated from umbilical-placental complexes (n = 3) with a mass of 66,5± 0,06 g received from the Tissue Bank. Preliminarily from the material, the non-collagenous proteins were removed with 0.05 M Na2HPO4, pH 8.7-9.1. The extraction was performed with pepsin and СН3СООН 0,5М and 5 mМ EDTA. Statistical processing was performed in Excel 2003. Results. The collagen extracts that were obtained after precipitation with 0.9% NaCl initially settled to the bottom of the container in the form of collagen fibers. After centrifugation and decantation of the supernatant, white mucilaginous substances were obtained which were purified by dialysis. The collagen concentrations that were obtained constituted 5,86± 0,04 mg/ml and were determined on the basis of dry mass. Conclusion. The amount of collagen obtained from the placental umbilical complex is significant and indicates that the source of production is a safe one and the method of production is efficient. Introducere. Substituenții osoși pe bază de colagen sunt utilizați pe scară largă în regenerarea țesutului osos în chirurgia ortopedică, traumatică și oromaxilo-facială. Sursele de extragere a colagenului predominant sunt de la animale, însă acestea pot transmite de la animal la om zoonoze. Astfel, colagenul din complexul ombilico-placentar prezintă un interes deosebit în utilizare ca grefă pentru regenerarea osoasă. Scopul lucrării. Obținerea colagenului din complexul ombilico-placentar pentru utilizarea în ingineria tisulară. Material și Metode. Colagenul a fost izolat din complexele ombilico-placentare (n=3) cu masa 66,5± 0,06 g primite de la Banca de Țesuturi. Preliminar, din material au fost îndepărtate proteinele necolagenice cu 0,05М Na2HPО4, pH 8,7-9,1. Extracția a fost efectuată cu pepsină și СН3СООН 0,5М și 5 mМ EDTA. Prelucrarea statistică a fost efectuată în Excel 2003. Rezultate. Extractele colagenice care au fost obținute după precipitarea cu NaCl 0,9 % inițial s-au sedimentat la fundul recipientului având aspect de fibre de colagen. După centrifugare și decantarea supernatantului au fost obținute substanțe mucilaginoase de culoare albă care au fost purificate prin dializă. Concentrațiile de colagen care au fost obținute au constituit 5,86± 0,04 mg/ml și au fost determinate în baza masei uscate. Concluzii. Cantitatea de colagen obținut din complexul ombilico-placentar este semnificativă și denotă că sursa de obținere este una sigură, iar metoda de obținere este eficientă

The man in the world of advanced information technologies: theoretical-philosophical and social aspects

Catedra Filosofie şi Bioetică a USMF „Nicolae Testemiţanu”Developments in the information society are one of the main agents of change worldwide. The complexity of this problem requires a man to examine some aspects, highlighting in particular, the theoretical-philosophical and social aspect. The article examines the manner in which this development could contribute to improving the human condition, determined him on such a man to be able to confront the acute problems of the society and to understand just how long the role that it plays technologies performance information in daily activity. Evoluţia societăţii informaţionale este unul dintre principalii agenţi ai schimbării planetare. Această complexitate a problemei îl impune pe om de a precăuta unele laturi, evidenţiind în special aspectul teoretico-filosofic, cît şi cel social. În articol se examinează modalitatea în care această evoluţie ar putea contribui la îmbunătăţirea considerabilă a condiţiei umane, determinîndu-l astfel pe om de a putea face faţă problemelor stringente din societate, cît şi de a înţelege rolul pe care-l joacă tehnologiile informaţionale performante în activitatea cotidiană

Lorin Cantemir – inginerul cu un CV demn de invidiat

În articolul dat conferențiarul universitar, poetul și Maestrul în Artă Victor Cobzac, aduce un omagiu profesorului universitar Lorin Cantemir: „O viață de Om trăită modest și cu respect către știință, către cei care l-au înconjurat zi de zi și către Dumnezeu și sfinții, cu care stă acum la o masă și ne veghează ca să nu facem pași greșiți în viața prea scurtă și al naibii de frumoasă. Un Om, care acolo unde a activat și-a lăsat inima în întregime, ca astăzi să-L pomenim la toate timpurile.

Environmental conception in the area of public health: theoretical and methodological configurations

Catedra Filosofie şi Bioetică, USMF „Nicolae Testemiţanu”The present civilization requires the need of a new configuration of the knowledge domain, where the branches of the future of science put in realization the sustainable way of human life by analysis of environmental conception, of innovation of ecological culture and of integration which is subject to technical control on the area of public health. The article examines the human intervention on nature to a high responsibility of humanity to contribute to creation of the conditions for a sustainable development and an adequate security of antroponoospheric society. Civilizaţia prezentă solicită necesitatea unei noi configuraţii a domeniului cunoaşterii, în care ramurile de viitor ale ştiinţei să pună în funcţiune modul durabil al vieţii umane prin intermediul analizei concepţiei invaironmentale, a inovaţiei culturii ecologice şi a integrării supuse controlului tehnicii asupra spaţiului sănătăţii publice. În articol se examinează intervenţia umană asupra naturii în vederea unei înalte responsabilităţi a umanităţii de a contribui la crearea condiţiilor pentru o dezvoltare durabilă şi o securizare adecvată a societăţii antroponoosferice

Human intervention - alternative explanation of environmental consciousness: theoretical and methodological issues

Catedra Filosofie şi Bioetică, USMF „Nicolae Testemiţanu”Confronting the present century civilization with a range of global issues is a primary topic in terms of solving and involvement in the common effort of humanity to save globe’s world population. The article examines the evolution of human actions on nature for adequate answers and solutions of civilization concern about the dangers directed to human health. Confruntarea civilizaţiei secolului prezent cu o serie de probleme globale constituie un subiect primordial în ce priveşte soluţionarea şi implicarea în efortul comun al umanităţii de a salva populaţia globului pămîntesc. În articol se examinează evoluţia acţiunilor umane asupra naturii în vederea unor răspunsuri şi soluţii adecvate ale civilizaţiei cu privire la preocuparea de pericole direcţionate asupra sănătăţii umane

Hepatocytes isolation from adult rats for liver recellularization

Tissue Engineering and Cells Cultures Laboratory, Nicolae Testemitsanu State University of Medicine and Pharmacy, Chisinau, the Republic of MoldovaBackground: Currently hepatocytes obtaining is prerequisite to create the necessary conditions for medical research, because it is an important tool in developing of new strategies in tissue engineering domain, which represents obtaining functional organs in laboratory conditions. Material and methods: The study was made on adult Wistar rats liver with body weight 274.66± 2.52 g (n=3) which were used for hepatocytes extraction by perfusion through the upper cave vein with combination of type II collagenase and type I dispase and Hank’s 0.9 mM MgCl2, 0.5 mM EDTA and 25 mM HEPES (HiMedia, India). Results: The cells were counted with trypan blue 0.25% in hemocytometer and cultured in William’s E medium (HiMedia, India) with 2 mM L-glutamine, 5% fetal bovine serum (Lonza, Belgium), antibiotic antimycotic solution (HiMedia, India), 100 nM dexamethasone and 100 nM insulin, with 2.5 x 105 cells per well in 12-well plates. After isolation were obtained 324, 48 ± 1, 25 x 106 hepatocytes, with a viability of 94.7 ± 0.9 % which indicates a high yield of cells viability. Conclusions: The hepatocyte isolation method by liver perfusion with the combination of collagenase-dispase is feasible for obtaining a large amount of functional hepatocytes intended for the recellularization in vitro of decellularized liver scaffolds. The yield and viability of hepatic cells could be increased by enzymatic digestion of liver tissue using combination of collagenase/dispase solution due to the less cytotoxic effect

The cohesion between environmental ethics and biodiversity – the objective of protecting of the heritage of human existence

Catedra Filosofie şi Bioetică, USMF „Nicolae Testemiţanu” din RMThe article examines an analysis from the perspective of bioethics on the scientific findings that are confered to the problems with reference to biodiversity. It is attempting an awarness of the complexity of the concerns in the way that are interconnected the environmental ethics and biodiversity, given the case that the precarious situation of the present it makes the threats to the adress of biodiversity loss with ethical direct implications over the entire of the planet and that requires a mandatory requirement of protection of the environment not to damage irreversibly the health and the life of mankind. În articol se examinează o analiză din perspectivă bioetică asupra constatărilor ştiinţifice care se conferă problemelor cu referire la biodiversitate. Se încearcă o conştientizare a complexităţii dilemelor în modul în care sunt interconectate etica invaironmentală şi biodiversitatea, dat fiind cazul că situaţia precară din prezent condiţionează ameninţări la adresa pierderii biodiversităţii cu implicaţii etice directe asupra întregii planete şi care necesită o cerinţă imperativă a protejării mediului de a nu prejudicia ireversibil sănătatea şi viaţa omenirii

An efficient procedure of isolation, cultivation and identification of bone marrow mesenchymal stem cells

Tissue Engineering and Cells Cultures Laboratory, Nicolae Testemitsanu State University of Medicine and Pharmacy, Chisinau, the Republic of MoldovaBackground: Bone marrow mesenchymal stem cells (MSC) have a wide application in domain of Regenerative Medicine. Of a great importance is utilization of a suitable bone marrow extraction technique that can provide a sufficient number of MSC to perform laboratory tests without seriously affecting the health of the laboratory animal. At the same time, before using in researches and clinical application, the MSC needs to be identified. Material and methods: The study was conducted in rabbits (n = 9), in which, from one iliac bone, by aspiration were taken 3.39 ± 1.27 ml of bone marrow. The nucleated bone marrow cells were separated through centrifugation using concentration gradient. The specific for stem cells culture medium was used, and MSC were multiplied during 2 passages. From the obtained MSC, 1x106 cells were subject to differentiation by chondrocytes lineage for other 20 days. The obtained chondrocytes aggregates were morphologically examined by Hematoxylin–Eosin staining and specific cartilage staining with Safranin O and Toluidine blue/fast green. Results: There was a strong correlation between the volume of collected bone marrow and the time required to achieve a 70-80% of MSC confluence (p=0.01). Also, the MSC isolated from bone marrow extracted from rabbit iliac bone were differentiated successful on chondrocyte line in all cases, confirmed through the specific cartilage staining with Safranin O and Toluidine blue/fast green (p<0,001). Conclusions: The volume of 3.39 ± 1.27 ml of bone marrow, harvested from rabbit iliac bone is sufficient to obtain a large number of MSC for the laboratory tests in vitro and in vivo. As a standard method for MSC identification could be used just the capability of the cells to differentiate in the specialized cell, including chondrocytes

FISH ROE SAMPLE PREPARATION FOR SYNTHETIC FOOD DYES DETERMINATION BY HPTLC

Food dyes are widely used in food products to offset the colour loss. To prevent fraud in the food industry, to ensure food safety and consumer health protection, it is necessary to implement fast, accurate and reliable methods for the analysis of dyes. A relatively fast method based on high-performance thin-layer chromatography (HPTLC) - photodensitometry was developed and applied to determine two synthetic food dyes (Sunset Yellow – E 110 and Ponceau 4R – E 124) in fish roe. TLC separation was carried out on silica gel plates, using the mixture isopropyl alcohol-ammonia (2:1, V/V) as mobile phase. Good separation of the dyes was achieved, the Rf values being 0.39 for E110 and 0.12 for E124, respectively. Due to the complexity of the matrix, sample preparation was performed into two steps: (i) extraction by ultrasound-assisted extraction with methanol-ammonia (1:1, V/V) and (ii) purification of the extract by ion-pair solid phase extraction (IP-SPE) on ChromaBond RP-18E cartridges. For both dyes, the overall recovery for the sample preparation step was higher than 91%, the relative standard deviation being less than 3.5%. The procedure was applied for E110 and E124 determination from a “red caviar” sample with the declared content of dyes