144 research outputs found
Linkage of an ABCC transporter to a single QTL that controls Ostrinia nubilalis larval resistance to the Bacillus thuringiensis Cry1Fa toxin
AbstractField evolved resistance of insect populations to Bacillus thuringiensis (Bt) crystalline (Cry) toxins expressed by crop plants has resulted in reduced control of insect feeding damage to field crops, and threatens the sustainability of Bt transgenic technologies. A single quantitative trait locus (QTL) that determines resistance in Ostrinia nubilalis larvae capable of surviving on reproductive stage transgenic corn that express the Bt Cry1Fa toxin was previously mapped to linkage group 12 (LG12) in a backcross pedigree. Fine mapping with high-throughput single nucleotide polymorphism (SNP) anchor markers, a candidate ABC transporter (abcc2) marker, and de novo mutations predicted from a genotyping-by-sequencing (GBS) data redefined a 268.8 cM LG12. The single QTL on LG12 spanned an approximate 46.1 cM region, in which marker 02302.286 and abcc2 were ≤2.81 cM, and the GBS marker 697 was an estimated 1.89 cM distant from the causal genetic factor. This positional mapping data showed that an O. nubilalis genome region encoding an abcc2 transporter is in proximity to a single QTL involved in the inheritance of Cry1F resistance, and will assist in the future identification the mutation(s) involved with this phenotype
Uniform bounds for higher-order semilinear problems in conformal dimension
We establish uniform a-priori estimates for solutions of the semilinear
Dirichlet problem \begin{equation} \begin{cases} (-\Delta)^m
u=h(x,u)\quad&\mbox{in }\Omega,\\
u=\partial_nu=\cdots=\partial_n^{m-1}u=0\quad&\mbox{on }\partial\Omega,
\end{cases} \end{equation} where is a positive superlinear and subcritical
nonlinearity in the sense of the Trudinger-Moser-Adams inequality, either when
is a ball or, provided an energy control on solutions is prescribed,
when is a smooth bounded domain. The analogue problem with Navier
boundary conditions is also studied. Finally, as a consequence of our results,
existence of a positive solution is shown by degree theory.Comment: Minor correction
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