95 research outputs found
Identification of markers associated with bacterial blight resistance loci in cowpea (Vigna unguiculata (L.) Walp.)
Cowpea bacterial blight (CoBB), caused by Xanthomonas axonopodis pv. vignicola (Xav), is a worldwide major disease of cowpea [Vigna unguiculata (L.) Walp.]. Among different strategies to control the disease including cultural practices, intercropping, application of chemicals, and sowing pathogen-free seeds, planting of cowpea genotypes with resistance to the pathogen would be the most attractive option to the resource poor cowpea farmers in sub-Saharan Africa. Breeding resistance cultivars would be facilitated by marker-assisted selection (MAS). In order to identify loci with effects on resistance to this pathogen and map QTLs controlling resistance to CoBB, eleven cowpea genotypes were screened for resistance to bacterial blight using 2 virulent Xav18 and Xav19 strains isolated from Kano (Nigeria). Two cowpea genotypes Danila and Tvu7778 were identified to contrast in their responses to foliar disease expression following leaf infection with pathogen. A set of recombinant inbred lines (RILs) comprising 113 individuals derived from Danila (resistant parent) and Tvu7778 (susceptible parent) were infected with CoBB using leaf inoculation method. The experiments were conducted under greenhouse conditions (2007 and 2008) and disease severity was visually assessed using a scale where 0 = no disease and 4 = maximum susceptibility with leaf drop. A single nucleotide polymorphism (SNP) genetic map with 282 SNP markers constructed from the same RIL population was used to perform QTL analysis. Using Kruskall-Wallis and Multiple-QTL model of MapQTL 5, three QTLs, CoBB-1, CoBB-2 and CoBB-3 were identified on linkage group LG3, LG5 and LG9 respectively showing that potential resistance candidate genes cosegregated with CoBB resistance phenotypes. Two of the QTLs CoBB-1, CoBB-2 were consistently confirmed in the two experiments accounting for up to 22.1 and to 17.4% respectively for the first and second experiments. Whereas CoBB-3 was only discovered for the first experiment (2007) with less phenotypic variation explained of about 10%. Our results represent a resource for molecular marker development that can be used for marker assisted selection of bacterial blight resistance in cowpe
Gene pools and the genetic architecture of domesticated cowpea
Open Access JournalCowpea [Vigna unguiculata (L.) Walp.] is a major tropical legume crop grown in warm to hot areas throughout the world and especially important to the people of sub-Saharan Africa where the crop was domesticated. To date, relatively little is understood about its domestication origins and patterns of genetic variation. In this study, a worldwide collection of cowpea landraces and African ancestral wild cowpea was genotyped with more than 1200 single nucleotide polymorphism markers. Bayesian inference revealed the presence of two major gene pools in cultivated cowpea in Africa. Landraces from gene pool 1 are mostly distributed in western Africa while the majority of gene pool 2 are located in eastern Africa. Each gene pool is most closely related to wild cowpea in the same geographic region, indicating divergent domestication processes leading to the formation of two gene pools. The total genetic variation within landraces from countries outside Africa was slightly greater than within African landraces. Accessions from Asia and Europe were more related to those from western Africa while accessions from the Americas appeared more closely related to those from eastern Africa. This delineation of cowpea germplasm into groups of genetic relatedness will be valuable for guiding introgression efforts in breeding programs and for improving the efficiency of germplasm managemen
Vector and Scalar Meson Resonances in Decays
Corrections to induced by vector and scalar meson
exchange are investigated within chiral perturbation theory.Comment: 15pages, Latex-file, TUM-T31-41/9
Phenomenology of Pc(4380)+, Pc(4450)+ and related states
The and states recently discovered at LHCb have
masses close to several relevant thresholds, which suggests they can be
described in terms of meson-baryon degrees of freedom. This article explores
the phenomenology of these states, and their possible partners, from this point
of view. Competing models can be distinguished by the masses of the neutral
partners which have yet to be observed, and the existence or otherwise of
further partners with different isospin, spin, and parity. Future experimental
studies in different decay channels can also discriminate among models, using
selection rules and algebraic relations among decays. Among the several
possible meson-baryon pairs which could be important, one implies that the
states are mixtures of isospins 1/2 and 3/2, with characteristic signatures in
production and decay. A previous experimental study of a Cabibbo-suppressed
decay showed no evidence for the states, and further analysis is required to
establish the significance of this non-observation. Several intriguing
similarities suggest that is related to the meson.Comment: 16 pages, 1 figure. Journal version (some very minor changes from
arXiv v1
Parton content of the real photon: astrophysical implications
We possess convincing experimental evidence for the fact that the real photon
has non-trivial parton structure. On the other hand, interactions of the cosmic
microwave background photons with high energy particles propagating through the
Universe play an important role in astrophysics. In this context, to invoke the
parton content could be convenient for calculations of the probabilities of
different processes involving these photons. As an example, the cross section
of inclusive resonant boson production in the reaction is calculated by using the parton language. Neutrino--photon deep
inelastic scattering is considered.Comment: 4 pages, 2 figures. The spin states of the initial particles in the
reaction are correctly treated. As a result, the
corresponding cross section becomes two times greater than the one from the
previous version. Some changes in the tex
Towards whole genome association genetic scans in barley
In crop plants, the potential of association mapping, with the objective of estimating the position of genes conferring a specific trait or phenotype using linkage disequilibrium (LD) between alleles of genetically mapped markers, has recently become a focus of considerable interest. One major attraction of association genetics is the potential to locate genes responsible for a wide range of traits in a single sample population using pre-existing phenotypic data that has been collected during crop improvement and cultivar registration programs. This study testify to the potential of exploiting whole genome LD-scans to locate genes controlling key biological traits in cultivated barley. We are currently increasing the density of markers, particularly those with a MAF >0.1, by developing two further pilot OPAs, which in due course will be compressed into two commercially available platforms for high throughput low cost genotyping in cultivated barley. In the immediate future these will be used in large association genetic studies in the UK and US involving approximately 4000 barley genotypes with the aim of realising the potential for whole genome association genetic scans in cultivated barley
The UCR Minicore: a valuable resource for cowpea research and breeding
Open Access Journal; Published online: 06 May 2021Incorporation of new sources of genetic diversity into plant breeding programs is crucial for continuing to improve yield and quality, as well as tolerance to abiotic and biotic stresses. A minicore (the “University of California, Riverside (UCR) Minicore”) composed of 368 worldwide accessions of cultivated cowpea has been assembled, having been derived from the UCR cowpea collection. High-density genotyping with 51,128 SNPs followed by principal component and genetic assignment analyses identified six subpopulations in the UCR Minicore, mainly differentiated by cultivar group and geographic origin. All six subpopulations were present to some extent in West African material, suggesting that West Africa is a center of diversity for cultivated cowpea. Additionally, population structure analyses supported two routes of introduction of cowpea into the U.S.: (1) from Spain to the southwest U.S. through Northern Mexico and (2) from Africa to the southeast U.S. via the Caribbean. Genome-wide association studies (GWAS) narrowed several traits to regions containing strong candidate genes. For example, orthologs of the Arabidopsis FLOWERING LOCUS T lie within a major QTL for flowering time. In summary, this diverse, yet compact cowpea collection constitutes a suitable resource to identify loci controlling complex traits, consequently providing markers to assist with breeding to improve this crop of high relevance to global food and nutritional security
High-contrast imaging constraints on gas giant planet formation - The Herbig Ae/Be star opportunity
Planet formation studies are often focused on solar-type stars, implicitly
considering our Sun as reference point. This approach overlooks, however, that
Herbig Ae/Be stars are in some sense much better targets to study planet
formation processes empirically, with their disks generally being larger,
brighter and simply easier to observe across a large wavelength range. In
addition, massive gas giant planets have been found on wide orbits around early
type stars, triggering the question if these objects did indeed form there and,
if so, by what process. In the following I briefly review what we currently
know about the occurrence rate of planets around intermediate mass stars,
before discussing recent results from Herbig Ae/Be stars in the context of
planet formation. The main emphasis is put on spatially resolved polarized
light images of potentially planet forming disks and how these images - in
combination with other data - can be used to empirically constrain (parts of)
the planet formation process. Of particular interest are two objects, HD100546
and HD169142, where, in addition to intriguing morphological structures in the
disks, direct observational evidence for (very) young planets has been
reported. I conclude with an outlook, what further progress we can expect in
the very near future with the next generation of high-contrast imagers at 8-m
class telescopes and their synergies with ALMA.Comment: Accepted by Astrophysics and Space Science as invited short review in
special issue about Herbig Ae/Be stars; 12 pages incl. 5 figures, 2 tables
and reference
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