第718回 千葉医学会例会・第二内科例会 14.

No correlation between pThr514 CRMP2 and pSer396 tau in LBD parietal cortex. a Bar graph of mean (± SEM) pSer396 tau to total tau ratios. Scatter plots of pThr514 CRMP2 with pSer396 tau : total tau in total homogenate fractions of b LBD (DLB + PDD), c DLB and d PDD parietal cortex, with insets indicating rho and p values. Available N for control = 19; PDD = 19 and DLB = 20. No significant differences (p > 0.05) were found for multiple pair-wise comparisons of pSer396 : total tau between groups (Kruskal-Wallis H tests), or for pThr514 CRMP2 correlations with pSer396 tau : total tau ratios (Spearman). (PDF 106 kb

Table_1_Physical and mental health conditions account for variability in awareness of age-related changes.docx

BackgroundThe concept of Awareness of Age-Related Changes captures people’s perceptions of the positive (AARC-gains) and negative (AARC-losses) age-related changes they experience in several life domains, including their health. We investigated the cross-sectional associations of number and type of physical and mental health conditions with AARC-gains and AARC-losses.MethodsThe sample comprised 3,786 middle-aged and older adults (mean age = 67.04 years; SD = 6.88) participating to the UK PROTECT study. We used hierarchical regression models to analyze whether after having included sociodemographic variables (model 1), number of physical (model 2) and of mental (model 3) health conditions explained a significant additional amount of variance in AARC-gains and AARC-losses, and whether the association between number of conditions and AARC depended on participants’ age. We used multiple regression models to analyze the associations of types of physical and mental health conditions with AARC-gains and AARC-losses.ResultsA higher number of physical health conditions was associated with higher AARC-gains and higher AARC-losses, but the association did not depend on participant age. After controlling for the number of physical health conditions, a higher number of mental health conditions was associated with higher AARC-losses but not with AARC-gains, and the association was stronger among older participants. Small effects were found between greater AARC-gains and current cancer and between greater AARC-losses and diagnoses of mild cognitive impairment, Parkinson’s disease, arthritic condition, cancer in full remission, osteoporosis, depression, anxiety disorders, and personality disorder. The remaining health conditions were either negligibly or non-statistically related to AARC-losses.ConclusionMiddle-aged and older adults having more physical health conditions and more mental health conditions may be at higher risk of negative views on their own aging. However, specific physical health conditions, such as arthritis, and certain mental health conditions, such as depression, may make adults particularly vulnerable to negative age-related perceptions.</p

Additional file 5: Figure S5. of Increased phosphorylation of collapsin response mediator protein-2 at Thr514 correlates with β-amyloid burden and synaptic deficits in Lewy body dementias

No correlation between pThr514 CRMP2 and α-synuclein immunoreactivity in LBD parietal cortex. a Bar graph of α-synuclein immunoreactivity (mean ± SEM in arbitrary units) with representative immunoblots, with GAPDH as loading control. Scatter plots of pThr514 CRMP2 with α-synuclein immunoreactivity in total homogenate fractions of b LBD (DLB + PDD), c DLB and d PDD parietal cortex, with insets indicating rho and p values. Available N for control (C) = 19; PDD (P) = 19 and DLB (D) = 20. No significant differences (p > 0.05) were found for multiple pair-wise comparisons of α-synuclein between groups (one-way ANOVA with Bonferroni’s post-hoc tests), or for pThr514 CRMP2 correlations with α-synuclein (Spearman). (PDF 195 kb

Additional file 6: Figure S6. of Increased phosphorylation of collapsin response mediator protein-2 at Thr514 correlates with β-amyloid burden and synaptic deficits in Lewy body dementias

No correlation between pThr514 CRMP2 and insoluble pSer129 α-synuclein immunoreactivity in LBD parietal cortex. a Bar graphs of pSer129 α-synuclein normalized to α-synuclein immunoreactivities in the insoluble fraction (mean ± SEM in arbitrary units) with representative immunoblots. Scatter plots of pThr514 CRMP2 with pSer129 α-synuclein immunoreactivity of b LBD (DLB + PDD), c DLB and d PDD parietal cortex, with insets indicating rho and p values. Available N for control (C) = 19; PDD (P) = 19 and DLB (D) = 19. One DLB sample was excluded as an outlier due to pSer129 α-synuclein value > 8, the inclusion of which did not alter the significance of the results (data not shown). *p < 0.05, **p < 0.01, significant differences for multiple pair-wise comparisons (Kruskal-Wallis H with Dunn’s post-hoc tests). No significant differences (p > 0.05) were found for pThr514 CRMP2 correlations with pSer129 α-synuclein (Spearman). (PDF 86 kb

Is lifetime traumatic brain injury a risk factor for mild cognitive impairment in veterans compared to non-veterans?

Background: Traumatic brain injury (TBI) is prevalent in veterans and may occur at any stages of their life (before, during, or after military service). This is of particular concern, as previous evidence in the general population has identified TBI as a strong risk factor for mild cognitive impairment (MCI), a known precursor of dementia. Objectives: This study aimed to investigate whether exposure to at least one TBI across the lifetime was a risk factor for MCI in ageing UK veterans compared to non-veterans. Method: This cross-sectional study comprised of data from PROTECT, a cohort study comprising UK veterans and non-veterans aged ≥ 50 years at baseline. Veteran and TBI status were self-reported using the Military Service History Questionnaire (MSHQ) and the Brain Injury Screening Questionnaire (BISQ), respectively. MCI was the outcome of interest, and was defined as subjective cognitive impairment and objective cognitive impairment. Results: The sample population comprised of veterans (n = 701) and non-veterans (n = 12,389). TBI was a significant risk factor for MCI in the overall sample (OR = 1.21, 95% CI 1.11–1.31) compared to individuals without TBI. The prevalence of TBI was significantly higher in veterans compared to non-veterans (69.9% vs 59.5%, p  Conclusion: TBI remains an important risk factor for MCI, irrespective of veteran status. The clinical implications indicate the need for early intervention for MCI prevention after TBI. Data from the PROTECT study, a longitudinal study comprising over 25,000 middle-aged and ageing adults in the UK, were used in this first UK comparative study to explore the association between a lifetime history of traumatic brain injury (TBI) and mild cognitive impairment (MCI) in UK veterans and non-veterans.Lifetime TBI was more prevalent in veterans compared to non-veterans. TBI events in military veterans could be attributed to non-military events.Exposure to a history of TBI irrespective of veteran status increased the risk of MCI by 21% compared to adults with no history of TBI.The risk of MCI did not significantly differ between veterans and non-veterans with TBI. Data from the PROTECT study, a longitudinal study comprising over 25,000 middle-aged and ageing adults in the UK, were used in this first UK comparative study to explore the association between a lifetime history of traumatic brain injury (TBI) and mild cognitive impairment (MCI) in UK veterans and non-veterans. Lifetime TBI was more prevalent in veterans compared to non-veterans. TBI events in military veterans could be attributed to non-military events. Exposure to a history of TBI irrespective of veteran status increased the risk of MCI by 21% compared to adults with no history of TBI. The risk of MCI did not significantly differ between veterans and non-veterans with TBI.</p

Additional file 1: Figure S1. of Increased phosphorylation of collapsin response mediator protein-2 at Thr514 correlates with β-amyloid burden and synaptic deficits in Lewy body dementias

CRMP2 is concentrated in cytosol-enriched fractions of postmortem human neocortex. Representative CRMP2 immunoblots of total and cytosol-enriched (“Cytosolic”) fractions of postmortem human neocortex (5 μg protein loaded per lane). β-actin was used as a control to show concentration of soluble cytoskeletal proteins, including CRMP2, in the cytosol-enriched fractions using the fractionation procedure described in Methods. (PDF 116 kb

Baseline, 6 and 12 week means and mean differences of memantine versus placebo using last outcome carried forward adjusted for baseline (except CGI as no baseline).

<p>The effect at weeks 6 & 12 uses lme and adjusts for baseline for CMAI, NPI, SIB and SMMSE.* p<0.05 ** p <0.01 *** P<0.001. NPI =  Neuropsychiatric Inventory; CGIC = Clinical Global Impression of change; SMMSE =  Standard Mini Mental State examination; CMAI =  Cohen Mansfield Agitation Index; SIB =  Severe Impairment Battery.</p

Baseline demographic and clinical values at randomisation according to study group.

<p>Baseline demographic and clinical values at randomisation according to study group.</p

CONSORT STATEMENT.

<p>CONSORT STATEMENT.</p

Mean (+/−95% confidence intervals) total CMAI by time and by group.

<p>Circles are individual data points; red: memantine, blue: placebo.</p