Emprego de diferentes estrategias para analises em larga escala : screening, extração ultra-sonica e pre-concentração por ponto nuvem

Orientador : Marco Aurelio Zezzi ArrudaTese (doutorado) - Universidade Estadual de Campinas, Instituto de QuimicaDoutorad

On-line Micro-solid Phase Preconcentration Of Cd2+ Coupled To Ts-ff-aas Using A Novel Ion-selective Bifunctional Hybrid Imprinted Adsorbent

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)A novel hybrid ion imprinted polymer (HIIP) is proposed aiming at the development of an online micro-solid phase preconcentration system of Cd2+ coupled to TS-FF-MS. To evaluate the selective and adsorptive performance of HIIP towards Cd2+, hybrid non-imprinted polymer (HNIP), organic ion imprinted polymer (OIIP), and the inorganic ion imprinted polymer (IIIP) were synthesized and compared with each other. Adsorption capacity of HIIP was 53% higher than HNIP, and dual-site Langmuir-Freundlich isotherm model showed the best fit for data of both polymers. The preconcentration system coupled to TS-FF-MS was performed by loading 10.0 mL of Cd2+ solution at pH 7.5 through 80.0 mg of HIIP packed into a micro-column with posterior online elution using 1.0 mol L-1 HCl/ethanol 1:1 (v/v) mixture. The developed method was highly tolerant for other metal ions Zn2+, Pb2+, Cu2+, Co2+, Fe2+, and Hg2+ at analyte:interferent (1:50, m/m) ratio and Ca2+ and Mg2+ at 1:500 (m/m) ratio. Analytical curve ranging from 0.5 to 7.0 mu g L-1, limit of detection of 30 mu g L-1 and preconcentration factor of 14-fold were obtained. Intra-day and inter-day (3 days) experiment precision (n =10) was, respectively, 3.9 and 0.6% (relative standard deviation, RSD), and 4.4 and 2.4% for concentrations of 0.7 and 6.5 mu g L-1. Sensitivity of method (Abs L mu g(-1)) was about 3.3,2.2 and 1.9-fold higher when compared to preconcentration method using HNIP, OIIP. and IIIP, respectively, thus clearly showing that hybrid polymer and imprinting process enhance Cd2+ adsorption. Addition and recovery experiments ranging from 94 to 106% in mineral, lake water and tap water, urine and cigarette samples attested the feasibility of method for analysis of matrices containing different components. Moreover, the accuracy of method was checked from analysis of certified reference material (DOLT-4, fish liver), being the obtained value of 23.9 +/- 0.6 mg kg(-1) (n = 3) statistically equal to the certified value of 24.3 +/- 0.8 mg kg(-1), by applying Student t-test at 95% confidence level. (C) 2016 Else Vier B.V. All rights reserved.1315769Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [481669/2013-2, 305552/2013-9, 472670/2012-3]Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [25/2014]Fundacao Araucaria do Parana [163/2014]Laborattorio de Espectroscopia da Central de Multiusuario da PROPPG, SANEPAR [09/2014]SETI do ParanaESPEC-UELInstituto Nacional de Ciencia e Tecnologia de Bioanalitica (INCT) [573672/2008-3]Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

Design of high-performance adsorption cross-linked organic functional polymers towards tricyclic antidepressants using computational simulation

Computational simulations of eighteen functional monomers to produce cross-linked polymers containing high adsorption efficiency to tricyclic antidepressants [amitriptyline (AMT), clomipramine (CLO), doxepin (DOX), imipramine (IMI) and nortriptyline (NOR)] in aqueous medium were evaluated and selected to polymers synthesis. Among them, acrylic acid (AA) and methacrylic acid (MA) were chosen to synthesize the poly(AA-co-EGDMA) and poly(MA-co-EGDMA) adsorbents due to their contrasting calculated interaction energies and to their similar chemical structures. The polymers were characterized by dynamic light scattering (DLS), elemental analysis, swelling effect and textural data. Higher tricyclic antidepressants adsorptions were obtained at pH 7.0 to both polymers, with superior results to poly(AA-co-EGDMA). From the adsorption kinetics the pseudo-second order, Elovich and intraparticle diffusion models showed good adjustments on experimental data of both polymers. The adsorptive predicted by the pseudo-second order model for the poly(AA-co-EGDMA) were 537, 480, 441, 522, 542 mu mol g(-1) to AMT, CLO, DOX, IMI and NOR, respectively, while to poly(MA-co-EGDMA) were 243, 120, 226, 205 and 178 mu mol g(-1). By the thermodynamic parameters, the adsorption process was characterized as endothermic and spontaneous, accompanied by an increase of disorder at the solid-solution interface. The higher adsorption capacity of poly(AA-co-EGDMA) to the antidepressants than to the poly(MA-co-EGDMA) demonstrates agreement with the interaction predicted by the computational simulation, showing its efficiency on predict the best functional monomer to synthesize polymeric adsorbents71CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO ARAUCÁRIA DE APOIO AO DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO DO ESTADO DO PARANÁ - FA481669/2013-2; 307432/2017-3; 472670/2012-33353/2014; 23038.007082/2014-03163/2014573672/2008-3INSTITUTO NACIONAL DE CIÊNCIA E TECNOLOGIA DE BIOLITÍTICA - INC

Desempenho de cultivares de alface tipo crespa sob altas temperaturas em Cáceres-MT

O cultivo de alface nas regiões tropicais tem sido dificultado pelas altas temperaturas e ampla luminosidade, afetando substancialmente o ciclo e a produção da cultura. Com o objetivo de avaliar o comportamento de cultivares de alface crespa, um experimento foi conduzido com 14 cultivares no delineamento experimental em blocos casualizados, com quatro repetições. As plantas foram arranjadas no esquema de quinquôncio, espaçadas a cada 0,30 metros e 0,25 metros, em parcelas contendo 20 plantas. Foram avaliadas a massa fresca total e comercial, massa seca, diâmetro da planta, número de folhas por planta, comprimento do caule e indícios de pendoamento nas cinco plantas centrais de cada parcela. Verificou-se que as cultivares Cinderela, Marisa e Elba são promissoras para o cultivo na região, no período de outubro à novembro, apesar de terem apresentado uma leve tendência ao alongamento do caule. Outra cultivar interessante avaliada foi a Simpson Elite, pois apresentou boa produção de massa fresca, diâmetro da planta, além de um menor tamanho do caule, mostrando tolerância á temperaturas mais elevadas. Em relação à característica comprimento do caule que indica resistência ao pendoamento, conclui-se que as cultivares que apresentam o melhor desempenho são as cultivares Vanda e Isabela, destacando que estas podem ser mantidas por um período maior no campo