Reproducible hairy root transformation and spot-inoculation methods to study root symbioses of pea

Pea has lagged behind other model legumes in the molecular study of nodulation and mycorrhizae-formation because of the difficulty to transform its roots and its poor growth on agar plates. Here we describe for pea 1) a transformation technique which permits the complementation of two known non-nodulating pea mutants, 2) a rhizobial inoculation method which allows the study of early cellular events giving rise to nodule primordia, and 3) a targeted fungal inoculation method which allows us to study short segments of mycorrhizal roots assured to be infected. These tools are certain to advance our knowledge of pea root symbioses

Dimensioning and Modulation Index Selection for the Hybrid Modular Multilevel Converter

The Hybrid MMC, comprising a mixture of fullbridge and half-bridge sub-modules, provides tolerance to DC faults without compromising the efficiency of the converter to a large extent. The inclusion of full-bridges creates a new freedom over the choice of ratio of AC to DC voltage at which the converter is operated, with resulting impact on the converter’s internal voltage, current and energy deviation waveforms, all of which impact the design of the converter. A design method accounting for this, and allowing the required level of derating of nominal sub-module voltage and up-rating of stack voltage capability to ensure correct operation at the extremes of the operating envelope is presented. A mechanism is identified for balancing the peak voltage that the full-bridge and halfbridge sub-modules experience over a cycle. Comparisons are made between converters designed to block DC side faults and converters that also add STATCOM capability. Results indicate that operating at a modulation index of 1.2 gives a good compromise between reduced power losses and additional required sub-modules and semiconductor devices in the converter. The design method is verified against simulation results and the operation of the converter at the proposed modulation index is demonstrated at laboratory-scale

The Extended Overlap Alternate Arm Converter:A Voltage Source Converter with DC Fault Ride-Through Capability and a Compact Design

The Alternate Arm Converter (AAC) was one of the first modular converter topologies to feature DC-side fault ride-through capability with only a small penalty in power efficiency. However, the simple alternation of its arm conduction periods (with an additional short overlap period) resulted in (i) substantial 6-pulse ripples in the DC current waveform, (ii) large DC-side filter requirements, and (iii) limited operating area close to an energy sweet-spot. This paper presents a new mode of operation called Extended Overlap (EO) based on the extension of the overlap period to 60 ◦ which facilitates a fundamental redefinition of the working principles of the AAC. The EO-AAC has its DC current path decoupled from the AC current paths, a fact allowing (i) smooth DC current waveforms, (ii) elimination of DC filters, and (iii) restriction lifting on the feasible operating point. Analysis of this new mode and EO- AAC design criteria are presented and subsequently verified with tests on an experimental prototype. Finally, a comparison with other modular converters demonstrates that the EO-AAC is at least as power efficient as a hybrid MMC (i.e. a DC fault ride-through capable MMC) while offering a smaller converter footprint because of a reduced requirement for energy storage in the submodules and a reduced inductor volume

Cascaded- and Modular-Multilevel Converter Laboratory Test System Options: A Review

The increasing importance of cascaded multilevel converters (CMCs), and the sub-category of modular multilevel converters (MMCs), is illustrated by their wide use in high voltage DC connections and in static compensators. Research is being undertaken into the use of these complex pieces of hardware and software for a variety of grid support services, on top of fundamental frequency power injection, requiring improved control for non-traditional duties. To validate these results, small-scale laboratory hardware prototypes are often required. Such systems have been built by many research teams around the globe and are also increasingly commercially available. Few publications go into detail on the construction options for prototype CMCs, and there is a lack of information on both design considerations and lessons learned from the build process, which will hinder research and the best application of these important units. This paper reviews options, gives key examples from leading research teams, and summarizes knowledge gained in the development of test rigs to clarify design considerations when constructing laboratory-scale CMCs.This work was supported in part by The University of Manchester supported by the National Innovation Allowance project ``VSC-HVDC Model Validation and Improvement'' and Dr. Heath's iCASE Ph.D. studentship supported through Engineering and Physical Sciences Research Council (EPSRC) and National Grid, in part by the Imperial College London supported by EPSRC through the HubNet Extension under Grant EP/N030028/1, in part by an iCASE Ph.D. Studentship supported by EPSRC and EDF Energy and the CDT in Future Power Networks under Grant EP/L015471/1, in part by University of New South Wales (UNSW) supported by the Solar Flagships Program through the Education Infrastructure Fund (EIF), in part by the Australian Research Council through the Discovery Early Career Research Award under Grant DECRA_DE170100370, in part by the Basque Government through the project HVDC-LINK3 under Grant ELKARTEK KK-2017/00083, in part by the L2EP research group at the University of Lille supported by the French TSO (RTE), and in part by the Hauts-de-France region of France with the European Regional Development Fund under Grant FEDER 17007725

Engaging diverse underserved communities to bridge the mammography divide

<p>Abstract</p> <p>Background</p> <p>Breast cancer screening continues to be underutilized by the population in general, but is particularly underutilized by traditionally underserved minority populations. Two of the most at risk female minority groups are American Indians/Alaska Natives (AI/AN) and Latinas. American Indian women have the poorest recorded 5-year cancer survival rates of any ethnic group while breast cancer is the number one cause of cancer mortality among Latina women. Breast cancer screening rates for both minority groups are near or at the lowest among all racial/ethnic groups. As with other health screening behaviors, women may intend to get a mammogram but their intentions may not result in initiation or follow through of the examination process. An accumulating body of research, however, demonstrates the efficacy of developing 'implementation intentions' that define when, where, and how a specific behavior will be performed. The formulation of intended steps in addition to addressing potential barriers to test completion can increase a person's self-efficacy, operationalize and strengthen their intention to act, and close gaps between behavioral intention and completion. To date, an evaluation of the formulation of implementation intentions for breast cancer screening has not been conducted with minority populations.</p> <p>Methods/Design</p> <p>In the proposed program, community health workers will meet with rural-dwelling Latina and American Indian women one-on-one to educate them about breast cancer and screening and guide them through a computerized and culturally tailored "implementation intentions" program, called <it>Healthy Living Kansas - Breast Health</it>, to promote breast cancer screening utilization. We will target Latina and AI/AN women from two distinct rural Kansas communities. Women attending community events will be invited by CHWs to participate and be randomized to either a mammography "implementation intentions" (<b>MI</b>^<b>2</b>) intervention or a comparison general breast cancer prevention informational intervention (<b>C</b>). CHWs will be armed with notebook computers loaded with our Healthy Living Kansas - Breast Health program and guide their peers through the program. Women in the <b>MI</b>^{<b>2 </b>}condition will receive assistance with operationalizing their screening intentions and identifying and addressing their stated screening barriers with the goal of guiding them toward accessing screening services near their community. Outcomes will be evaluated at 120-days post randomization via self-report and will include mammography utilization status, barriers, and movement along a behavioral stages of readiness to screen model.</p> <p>Discussion</p> <p>This highly innovative project will be guided and initiated by AI/AN and Latina community members and will test the practical application of emerging behavioral theory among minority persons living in rural communities.</p> <p>Trial Registration</p> <p>ClinicalTrials (NCT): <a href="http://www.clinicaltrials.gov/ct2/show/NCT01267110">NCT01267110</a></p

Effective anisotropic elastic constants of bimaterial interphases: comparison between experimental and analytical techniques

The effective elastic constants of a bimaterial composite were experimentally measured with the goal of validating the numerical predications of these constants made by homogenization theory. Secondly, solutions predicted by homogenization theory were compared to predictions made with more standard composite theories. Composite specimens consisting of titanium and epoxy were developed to mimic a porous titanium/tissue interphase. Tensile and shear tests (ASTM D3983) measured the stiffness along the porous coating/epoxy interphase ( E L ), across the interphase ( E T ) and in shear ( G LT ). No significant differences in moduli were found between the experimental measurements and predictions made with homogenization theory, nor between the experimental measurements and Hashin-Shtrikman estimates. Homogenization theory predicted results usually within 20% of Hashin-Shtrikman estimates, but typically more than 50% different from what is predicted by the rule of mixtures. However, homogenization theory allows calculation of anisotropic stiffness estimates and local strains, neither of which is possible using Hashin-Shtrikman estimates. With this experimental validation, the accuracy of homogenization theory for use in implant/tissue interface mechanics applications is confirmed. Since the composite interphase is anisotropic and more compliant in the transverse direction, with stiffness an order of magnitude lower across the interphase, local mechanics, tissue ingrowth and remodeling may be strongly directional dependent.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/46748/1/10856_2004_Article_BF00058722.pd

Experimental study on the influence of MMC control design on the propagation of AC grid-unbalance to a DC network

The Modular Multilevel Converter (MMC) has become the leading technology for delivering High Voltage DC (HVDC) power transmission, due to its scalability, harmonic quality and ability to ride through AC network faults. However, unbalanced AC-Grid conditions create harmonics at twice the fundamental AC-Grid frequency that can propagate to the DC-side as a result of the unbalance in the arm energy. These harmonics may risk excitation of network resonances as well as the maloperation of connected assets, and thus control action is needed to suppress such harmonics. Several different control solutions have been proposed in the literature and the aim of this paper is to provide a comparison of the performance of a sample of these techniques on a 1.2 kV/12 kVA lab-scale MMC demonstrator and cable emulator under unbalanced AC-Grid conditions. The sample of control methods chosen showcases a range of design complexity from basic direct modulation techniques with no DC current control to advanced energy based controllers. Experimental testing of these controllers under the same test condition not only validates the operation of each individual controller but also allows like-for-like comparison of their relative performance. It was found that the controllers tested were capable of significantly suppressing double line-frequency components on the DC -bus compared to the base case, however the more complex controllers had the additional benefit of being able to tune the transient response

Health behavior change among women with recent familial diagnosis of breast cancer

BACKGROUND: Although first-degree female relatives (FDFR) of women with breast cancer are at increased risk for the disease, little is understood about how familial diagnosis impacts health behaviors and what personal factors predict such changes. METHODS: Six hundred women, ages 18 and over with a FDFR recently diagnosed with breast cancer, were interviewed after the diagnosis and again in 6 months. Participants self-reported changes in physical activity, fruit and vegetable consumption, fat consumption, alcohol and tobacco use. The effect of baseline demographics, health status, perceptions of relative\u27s disease severity, personal risk, control over the disease and the effect of lifestyle behaviors on risk was assessed in relation to behavior changes. RESULTS: Forty-two percent reported improving one or more behaviors. Perception that the behavior was a risk factor for breast cancer was positively associated with change for all behaviors except smoking. Poor health status, obesity and perception of control over breast cancer were associated with improvements in physical activity, fruit and vegetable consumption and fat consumption. CONCLUSIONS: Diagnosis of breast cancer in a first-degree relative can provide motivation to improve health behavior. Educational interventions highlighting the importance of these behaviors in reducing breast cancer risk and promoting health in general may be effective in this population

A review of the efficacy of atomoxetine in the treatment of attention-deficit hyperactivity disorder in children and adult patients with common comorbidities

David B Clemow,1 Chris Bushe,2 Michele Mancini,3 Michael H Ossipov,4 Himanshu Upadhyaya1 1Eli Lilly and Company, Indianapolis, IN, USA; 2Eli Lilly, Windlesham, UK; 3Eli Lilly Italia S.p.A., Sesto Fiorentino, Italy; 4inVentiv Health Clinical, LLC, Blue Bell, PA, USA Abstract: Attention-deficit hyperactivity disorder (ADHD) is a common neuropsychiatric disorder that is often diagnosed during childhood, but has also increasingly been recognized to occur in adults. Importantly, up to 52% of children (including adolescents) and 87% of adults with ADHD also have a comorbid psychiatric disorder. The presence of a comorbid disorder has the potential to impact diagnosis and could affect treatment outcomes. Atomoxetine is a nonstimulant treatment for ADHD. Despite numerous published studies regarding efficacy of atomoxetine in the treatment of ADHD in patients with comorbid disorders, there is limited information about the impact of individual common comorbid disorders on the efficacy of atomoxetine for ADHD, especially with regard to adults. Moreover, a cumulative review and assessment of these studies has not been conducted. For this reason, we performed a literature review to find, identify, and cumulatively review clinical studies that examined the efficacy of atomoxetine in the treatment of patients with ADHD and comorbid psychiatric disorders. We found a total of 50 clinical studies (37 in children; 13 in adults) that examined the efficacy of atomoxetine in patients with ADHD and a comorbid disorder. The comorbidities that were studied in children or in adults included anxiety, depression, and substance use disorder. Overall, the presence of comorbidity did not&nbsp;adversely impact the efficacy of atomoxetine in treatment of ADHD symptoms in both patient populations. In the studies identified and assessed in this review, atomoxetine did not appear to exacerbate any of the comorbid conditions and could, therefore, be an important therapy choice for the treatment of ADHD in the presence of comorbid disorders. Keywords: comorbid psychiatric disorders, ADHD in children or adolescents, adult attention-deficit hyperactivity disorder, ADH