Use of a commercial enzyme immunoassay to monitor dengue virus replication in cultured cells

Current methods for dengue virus quantitation are either time consuming, technically demanding or costly. As an alternative, the commercial enzyme immunoassay Platelia™ Dengue NS1 AG (Bio-Rad Laboratories) was used to monitor semiquantitatively dengue virus replication in cultured cells. The presence of NS1 protein was evaluated in supernatants from Vero and C6/36 HT cells infected with dengue virus. The amount of NS1 detected in the supernatants of infected cells was proportional to the initial MOI used and to the time of post infection harvest. This immunoassay was also able to detect the presence of NS1 in the supernatants of infected human macrophages. Inhibition of dengue virus replication in C6/36 HT cells treated with lysosomotropic drugs was readily monitored with the use of this assay. These results suggest that the Platelia™ Dengue NS1 AG kit can be used as a fast and reliable surrogate method for the relative quantitation of dengue virus replication in cultured cells

Use of a commercial enzyme immunoassay to monitor dengue virus replication in cultured cells-0

Ent times post-infection, the media were collected and tested for the presence of NS1 protein with Platelia™ Dengue NS1 Ag kit. (C) Ten micro liters of the supernatant media collected from the experiments shown in (A) were mixed with 40 μl of diluent buffer provided with the kit and used to measure NS1 protein levels with Platelia™ Dengue NS1 Ag kit. Points in (A) and (B) are mean values ± SD of three independent experiments.<p><b>Copyright information:</b></p><p>Taken from "Use of a commercial enzyme immunoassay to monitor dengue virus replication in cultured cells"</p><p>http://www.virologyj.com/content/5/1/51</p><p>Virology Journal 2008;5():51-51.</p><p>Published online 25 Apr 2008</p><p>PMCID:PMC2386455.</p><p></p

Use of a commercial enzyme immunoassay to monitor dengue virus replication in cultured cells-4

Ere collected and used for: measurement of NS1 protein levels with Platelia™ Dengue NS1 Ag kit (open symbols) and for focus forming units (FFU) calculation (solid symbols). Points are mean values of three independent experiments.<p><b>Copyright information:</b></p><p>Taken from "Use of a commercial enzyme immunoassay to monitor dengue virus replication in cultured cells"</p><p>http://www.virologyj.com/content/5/1/51</p><p>Virology Journal 2008;5():51-51.</p><p>Published online 25 Apr 2008</p><p>PMCID:PMC2386455.</p><p></p

Use of a commercial enzyme immunoassay to monitor dengue virus replication in cultured cells-3

Ent times post-infection, the media were collected and tested for the presence of NS1 protein with Platelia™ Dengue NS1 Ag kit. (C) Ten micro liters of the supernatant media collected from the experiments shown in (A) were mixed with 40 μl of diluent buffer provided with the kit and used to measure NS1 protein levels with Platelia™ Dengue NS1 Ag kit. Points in (A) and (B) are mean values ± SD of three independent experiments.<p><b>Copyright information:</b></p><p>Taken from "Use of a commercial enzyme immunoassay to monitor dengue virus replication in cultured cells"</p><p>http://www.virologyj.com/content/5/1/51</p><p>Virology Journal 2008;5():51-51.</p><p>Published online 25 Apr 2008</p><p>PMCID:PMC2386455.</p><p></p

Use of a commercial enzyme immunoassay to monitor dengue virus replication in cultured cells-1

Ere collected and used for: measurement of NS1 protein levels with Platelia™ Dengue NS1 Ag kit (open symbols) and for focus forming units (FFU) calculation (solid symbols). Points are mean values of three independent experiments.<p><b>Copyright information:</b></p><p>Taken from "Use of a commercial enzyme immunoassay to monitor dengue virus replication in cultured cells"</p><p>http://www.virologyj.com/content/5/1/51</p><p>Virology Journal 2008;5():51-51.</p><p>Published online 25 Apr 2008</p><p>PMCID:PMC2386455.</p><p></p

Assessing the Potential Distributions of the Invasive Mosquito Vector Aedes albopictus and Its Natural Wolbachia Infections in México

The Asian tiger mosquito Aedes albopictus is currently the most invasive vector species, with a widespread global distribution. Aedes albopictus is the potential vector of diverse arboviruses, including Zika and dengue. This study updated the ecological niche model of Ae. albopictus and inferred the potential distribution of natural Wolbachia infections in Ae. albopictus in México. The ecological niche models were constructed based on diverse model settings to better estimate the potential distributions and uncertainty indices of both Ae. albopictus and its natural Wolbachia infections in México. The distribution of Ae. albopictus covered the states across Northern México, the Gulf of México, the Pacific Coast of México, Central México, and the southeast of México. The ecological niche model of the natural Wolbachia infections in Ae. albopictus populations anticipated the occurrence of natural Wolbachia infections in the southeast of México, the Chiapas border with Guatemala, and Veracruz. These results can be used to prioritize vector surveillance and control programs in México for strategic and future decision-making; however, it is still necessary to establish active surveillance programs to assess model predictions based on the independent sampling of Ae. albopictus from different invasion zones in México. Finally, vector surveillance should also screen the natural Wolbachia infections in Ae. albopictus to validate Wolbachia predictions across México, particularly in the southeast of México

Evidence that the 45 kDa glycoprotein, part of a putative dengue virus receptor complex in the mosquito cell line C6/36, is a heat shock-related protein

Artículo científic

Using demographic data to understand the distribution of H1N1 and COVID-19 pandemics cases among federal entities and municipalities of Mexico

Background The novel coronavirus disease (COVID-19) pandemic is the second global health emergency the world has faced in less than two decades, after the H1N1 Influenza pandemic in 2009–2010. Spread of pandemics is frequently associated with increased population size and population density. The geographical scales (national, regional or local scale) are key elements in determining the correlation between demographic factors and the spread of outbreaks. The aims of this study were: (a) to collect the Mexican data related to the two pandemics; (b) to create thematic maps using federal and municipal geographic scales; (c) to investigate the correlations between the pandemics indicators (numbers of contagious and deaths) and demographic patterns (population size and density). Methods The demographic patterns of all Mexican Federal Entities and all municipalities were taken from the database of “Instituto Nacional de Estadística y Geografía” (INEGI). The data of “Centro Nacional de Programas Preventivos y Control de Enfermedades” (CENAPRECE) and the geoportal of Mexico Government were also used in our analysis. The results are presented by means of tables, graphs and thematic maps. A Spearman correlation was used to assess the associations between the pandemics indicators and the demographic patterns. Correlations with a p value < 0.05 were considered significant. Results The confirmed cases (ccH1N1) and deaths (dH1N1) registered during the H1N1 Influenza pandemic were 72.4 thousand and 1.2 thousand respectively. Mexico City (CDMX) was the most affected area by the pandemic with 8,502 ccH1N1 and 152 dH1N1. The ccH1N1 and dH1N1 were positively correlated to demographic patterns; p-values higher than the level of marginal significance were found analyzing the % ccH1N1 and the % dH1N1 vs the population density. The COVID-19 pandemic data indicated 75.0 million confirmed cases (ccCOVID-19) and 1.6 million deaths (dCOVID-19) worldwide, as of date. The CDMX, where 264,330 infections were recorded, is the national epicenter of the pandemic. The federal scale did not allow to observe the correlation between demographic data and pandemic indicators; hence the next step was to choose a more detailed geographical scale (municipal basis). The ccCOVID-19 and dCOVID-19 (municipal basis) were highly correlated with demographic patterns; also the % ccCOVID-19 and % dCOVID-19 were moderately correlated with demographic patterns. Conclusion The magnitude of COVID-19 pandemic is much greater than the H1N1 Influenza pandemic. The CDMX was the national epicenter in both pandemics. The federal scale did not allow to evaluate the correlation between exanimated demographic variables and the spread of infections, but the municipal basis allowed the identification of local variations and “red zones” such as the delegation of Iztapalapa and Gustavo A. Madero in CDMX