Evaluation of the Graduate Leader Fund: Factors relating to quality: findings from the baseline study

This is the final version of the report. Available from the Department for Education via the link in this record.

Reinterpretation and Long-Term Preservation of Data and Code

Careful preservation of experimental data, simulations, analysis products, and theoretical work maximizes their long-term scientific return on investment by enabling new analyses and reinterpretation of the results in the future. Key infrastructure and technical developments needed for some high-value science targets are not in scope for the operations program of the large experiments and are often not effectively funded. Increasingly, the science goals of our projects require contributions that span the boundaries between individual experiments and surveys, and between the theoretical and experimental communities. Furthermore, the computational requirements and technical sophistication of this work is increasing. As a result, it is imperative that the funding agencies create programs that can devote significant resources to these efforts outside of the context of the operations of individual major experiments, including smaller experiments and theory/simulation work. In this Snowmass 2021 Computational Frontier topical group report (CompF7: Reinterpretation and long-term preservation of data and code), we summarize the current state of the field and make recommendations for the future.Comment: Snowmass 2021 Computational Frontier CompF7 Reinterpretation and long-term preservation of data and code topical group repor

High-Throughput Molecular Cancer Cell Line Characterization Using Digital Multiplex Ligation-Dependent Probe Amplification for Improved Standardization of in Vitro Research.

Tumor cell lines are widely used for cancer research, but challenges regarding quality control of cell line identity, cross contamination, and tumor somatic molecular stability remain, demanding novel approaches beyond conventional short tandem repeat profiling. A total of 21 commonly used multiple myeloma cell lines obtained from public repositories were analyzed by digital multiplex ligation-dependent probe amplification (digitalMLPA) to characterize germline single-nucleotide polymorphisms, insertions/deletions, and somatic copy number aberrations (CNAs). Using generated profiles and an in-house developed analytical pipeline, blinded experiments were performed to determine capability of digitalMLPA to predict cell line identity and potential spike-in DNA contamination in 41 anonymized cell line samples. The dominant cell line was correctly identified in all cases, and cross contamination was correctly detected in 33 of 37 samples with spike-in DNA; there were no false-positive predictions. The four samples in which spike in was not detected all carried low levels of contamination (1%), whereas levels of contamination ≥5% were correctly identified in all cases. Unsupervised clustering of CNA profiles identified shared commonalities that correlated with initiating Ig heavy locus translocation events. Longitudinal CNA assessment of nine cell lines revealed changes under standard culturing conditions not detected by insertion/deletion profiling alone. Results suggest that digitalMLPA can be utilized as a high-throughput tool for advanced quality assurance for in vitro cancer research

Role of Asian summer monsoon subsystems in the inter-hemispheric progression of deglaciation

The responses of Asian monsoon subsystems to both hemispheric climate forcing and external orbital forcing are currently issues of vigorous debate. The Indian summer monsoon is the dominant monsoon subsystem in terms of energy flux, constituting one of Earth’s most dynamic expressions of ocean–atmosphere interactions. Yet, the Indian summer monsoon is grossly under-represented in Asian monsoon palaeoclimate records. Here, we present high-resolution records of Indian summer monsoon- induced rainfall and fluvial runoff recovered in a sediment core from the Bay of Bengal across Termination II, 139–127 thousand years ago, including coupled measurements of the oxygen isotopic composition and Mg/Ca, Mn/Ca, Nd/Ca and U/Ca ratios in surface-ocean-dwelling foraminifera. Our data reveal a millennial-scale transient strengthening of the Asian monsoon that punctuates Termination II associated with an oscillation of the bipolar seesaw. The progression of deglacial warming across Termination II emerges first in the Southern Hemisphere, then the tropics in tandem with Indian summer monsoon strengthening, and finally the Northern Hemisphere. We therefore suggest that the Indian summer monsoon was a conduit for conveying Southern Hemisphere latent heat northwards, thereby promoting subsequent Northern Hemisphere deglaciation

Seasonal variation in hospital encounters with hypoglycaemia and hyperglycaemia

Aim To assess whether rates of hospital encounters with hypoglycaemia and hyperglycaemia display seasonal variation. Methods Time series analyses of the monthly rates of hospital encounters (emergency room visits or inpatient admissions) with hypoglycaemia and hyperglycaemia from 2003 to 2012 using linked healthcare databases in Ontario, Canada. Results Over the study period, there were 129 887 hypoglycaemia and 79 773 hyperglycaemia encounters. The characteristics of people at the time of their encounters were similar across the seasons in 2008 (median age 68 years for hypoglycaemia encounters and 53 years for hyperglycaemia encounters; 50% female; 90% with diabetes). We observed moderate seasonality in both types of encounters (R2 autoregression coefficient 0.58 for hypoglycaemia; 0.59 for hyperglycaemia). The rate of hypoglycaemia encounters appeared to peak between April and June, when on average, there was an additional 49 encounters per month (0.36 encounters per 100 000 persons per month) compared with the other calendar months (5% increase). The rate of hyperglycaemia encounters appeared to peak in January, when on average, there was an additional 69 encounters per month (0.50 encounters per 100 000 persons per month) compared with the other calendar months (11% increase). Conclusions In our region, there is seasonal variation in the rate of hospital encounters with hypoglycaemia and hyperglycaemia. Our findings may help to highlight periods of vulnerability for people, may inform future epidemiological studies and may aid in the appropriate planning of healthcare resources

A label-free, quantitative assay of amyloid fibril growth based on intrinsic fluorescence.

Kinetic assay of seeded growth: The graph shows the variation in intrinsic fluorescence intensity of amyloid fibrils. Fluorescence increases during the seeded aggregation of α-synuclein seeds with α-synuclein monomeric protein (blue curve) but not when α-synuclein seeds are incubated with β-synuclein monomeric protein (black curve), thus showing that no seeded growth occurred in this case

The stellar population histories of early-type galaxies. III. The Coma Cluster

We present stellar population parameters of twelve early-type galaxies (ETGs) in the Coma Cluster based on spectra obtained using the Low Resolution Imaging Spectrograph on the Keck II Telescope. Our data allow us to examine in detail the zero-point and scatter in their stellar population properties. Our ETGs have SSP-equivalent ages of on average 5-8 Gyr with the models used here, with the oldest galaxies having ages of ~10 Gyr old. This average age is identical to the mean age of field ETGs. Our ETGs span a large range in velocity dispersion but are consistent with being drawn from a population with a single age. Specifically, ten of the twelve ETGs are consistent within their formal errors of having the same age, 5.2+/-0.2 Gyr, over a factor of more than 750 in mass. We therefore find no evidence for downsizing of the stellar populations of ETGs in the core of the Coma Cluster. We suggest that Coma Cluster ETGs may have formed the majority of their mass at high redshifts but suffered small but detectable star formation events at z~0.1-0.3. Previous detections of 'downsizing' from stellar populations of local ETGs may not reflect the same downsizing seen in lookback studies of RSGs, as the young ages of the local ETGs represent only a small fraction of their total masses. (abridged)Comment: 49 pages, 20 figures (19 EPS, 1 JPEG). MNRAS, in press. For version with full resolution of Fig. 1 see http://www.astro.rug.nl/~sctrager/coma.pdf; for Table 2, see http://www.astro.rug.nl/~sctrager/coma_table2.pdf; for Table B3, see http://www.astro.rug.nl/~sctrager/coma_tableB3.pd

Paramagnetic GaN:Fe and ferromagnetic (Ga,Fe)N - relation between structural, electronic, and magnetic properties

We report on the metalorganic chemical vapor deposition (MOCVD) of GaN:Fe and (Ga,Fe)N layers on c-sapphire substrates and their thorough characterization via high-resolution x-ray diffraction (HRXRD), transmission electron microscopy (TEM), spatially-resolved energy dispersive X-ray spectroscopy (EDS), secondary-ion mass spectroscopy (SIMS), photoluminescence (PL), Hall-effect, electron-paramagnetic resonance (EPR), and magnetometry employing a superconducting quantum interference device (SQUID). A combination of TEM and EDS reveals the presence of coherent nanocrystals presumably FexN with the composition and lattice parameter imposed by the host. From both TEM and SIMS studies, it is stated that the density of nanocrystals and, thus the Fe concentration increases towards the surface. In layers with iron content x<0.4% the presence of ferromagnetic signatures, such as magnetization hysteresis and spontaneous magnetization, have been detected. We link the presence of ferromagnetic signatures to the formation of Fe-rich nanocrystals, as evidenced by TEM and EDS studies. This interpretation is supported by magnetization measurements after cooling in- and without an external magnetic field, pointing to superparamagnetic properties of the system. It is argued that the high temperature ferromagnetic response due to spinodal decomposition into regions with small and large concentration of the magnetic component is a generic property of diluted magnetic semiconductors and diluted magnetic oxides showing high apparent Curie temperature.Comment: 21 pages, 30 figures, submitted to Phys. Rev.