Perception of elderly people who live alone about their conditions of life and health / Percepção de idosos que moram sozinhos acerca de suas condições de vida e saúde

Objetivo: compreender a percepção de idosos que moram sozinhos acerca de suas condições de vida e saúde. Método: estudo qualitativo, realizado com 21 idosos que viviam sozinhos em João Pessoa, Paraíba, Brasil. Os dados foram coletados por meio de entrevistas, utilizando um instrumento semiestruturado contendo dados sociodemográficos e perguntas sobre condições de vida e de saúde, sendo processados no software Iramuteq, sendo utilizado o método da Classificação Hierárquica Descendente. Resultados: foram conformadas cinco classes ou categorias: O que leva o idoso morar sozinho; Riscos de queda para o idoso; Comorbidades dos idosos; Satisfação e insatisfação do idoso que mora só; e Atenção à saúde do idoso. Conclusão: conhecer as necessidades, os riscos e a vulnerabilidade dos idosos que moram sozinhos auxilia no planejamento de cuidado, com foco na prevenção de quedas e desgastes emocionais provenientes da solidão, no tratamento e reabilitação das doenças crônicas e suas consequências.

Characterisation of the binding site and electrophysiological effects of Bunodosoma granulifera

SIGLEAvailable from British Library Document Supply Centre- DSC:DXN055888 / BLDSC - British Library Document Supply CentreGBUnited Kingdo

Body in Motion, Street Dance y agencias del patrimonio cultural: una experiencia de investigación (Sobral-CE)

O presente estudo discute sobre a classificação da prática social da Dança de Rua/Street Dance, como patrimônio cultural, mais especificamente, tendo como delimitação a história do tempo presente e como método a prática de compartilhar experiências cotidianas com um grupo de dança denominado Street Dance - Jovens em Movimento – grupo este formado por jovens advindos da periferia da cidade brasileira de Sobral, localizada no norte do estado do Ceará.The present study discusses the classification of the social practice of Street Dance as a cultural heritage, more specifically, having as delimitation the history of the present time and as a method the practice of sharing daily experiences with a dance group called Street Dance. - Young People in Movement - group formed by young people coming from the outskirts of the Brazilian city of Sobral, located in the north of the state of Ceará.El presente estudio discute la clasificación de la práctica social de Street Dance como patrimonio cultural, más específicamente, teniendo como delimitación la historia de la actualidad y como método la práctica de compartir experiencias diarias con un grupo de baile llamado Street Dance. - Jóvenes en movimiento: grupo formado por jóvenes procedentes de las afueras de la ciudad brasileña de Sobral, ubicada en el norte del estado de Ceará

Mapping the functional anatomy of BgK on Kv1.1, Kv1.2, and Kv1.3. : Clues to design analogs with enhanced selectivity

BgK is a peptide from the sea anemone Bunodosoma granulifera, which blocks Kv1.1, Kv1.2, and Kv1.3 potassium channels. Using 25 analogs substituted at a single position by an alanine residue, we performed the complete mapping of the BgK binding sites for the three Kv1 channels. These binding sites included three common residues (Ser-23, Lys-25, and Tyr-26) and a variable set of additional residues depending on the particular channel. Shortening the side chain of Lys-25 by taking out the four methylene groups dramatically decreased the BgK affinity to all Kv1 channels tested. However, the analog K25Orn displayed increased potency on Kv1.2, which makes this peptide a selective blocker for Kv1.2 (K(D) 50- and 300-fold lower than for Kv1.1 and Kv1.3, respectively). BgK analogs with enhanced selectivity could also be made by substituting residues that are differentially involved in the binding to some of the three Kv1 channels. For example, the analog F6A was found to be >500-fold more potent for Kv1.1 than for Kv1.2 and Kv1.3. These results provide new information about the mechanisms by which a channel blocker distinguishes individual channels among closely related isoforms and give clues for designing analogs with enhanced selectivity

Delineation of the functional site of alpha-dendrotoxin - the functional topographies of dendrotoxins are different but share a conserved core with those of other kv1 potassium channel-blocking toxins

We identified the residues that are important for the binding of α-dendrotoxin (αDTX) to Kv1 potassium channels on rat brain synaptosomal membranes, using a mutational approach based on site-directed mutagenesis and chemical synthesis. Twenty-six of its 59 residues were individually substituted by alanine. Substitutions of Lys5 and Leu9 decreased affinity more than 1000-fold, and substitutions of Arg3, Arg4, Leu6, and Ile8 by 5-30-fold. Substitution of Lys5 by norleucine or ornithine also greatly altered the binding properties of αDTX. All of these analogs displayed similar circular dichroism spectra as compared with the wild-type αDTX, indicating that none of these substitutions affect the overall conformation of the toxin. Substitutions of Ser38 and Arg46 also reduced the affinity of the toxin but, in addition, modified its dichroic properties, suggesting that these two residues play a structural role. The other residues were excluded from the recognition site because their substitutions caused no significant affinity change. Thus, the functional site of αDTX includes six major binding residues, all located in its N-terminal region, with Lys5 and Leu9 being the most important. Comparison of the functional site of αDTX with that of DTX-K, another dendrotoxin (Smith, L. A., Reid, P. F., Wang, F. C., Parcej, D. N., Schmidt, J. J., Olson, M. A., and Dolly, J. O. (1997)Biochemistry 36, 7690-7696), reveals that they only share the predominant lysine and probably a leucine residue; the additional functional residues differ from one toxin to the other. Comparison of the functional site of αDTX with those of structurally unrelated potassium channel-blocking toxins from venomous invertebrates revealed the common presence of a protruding key lysine with a close important hydrophobic residue (Leu, Tyr, or Phe) and few additional residues. Therefore, irrespective of their phylogenetic origin, all of these toxins may have undergone a functional convergence. The functional site of αDTX is topographically unrelated to the 'antiprotease site' of the structurally analogous bovine pancreatic trypsin inhibitor