574 research outputs found
Thermal Stability of Polystyrene Latex Self-Assembled Arrays Studied by Atomic Force Microscopy
Preliminary observation of the heat softening of self-assembled polystyrene latex arrays on the submicron scale using atomic force microscopy is reported. Samples of latex self-assembled arrays have been studied in the temperature range from the room temperature up to 120°C. It has been shown that the submicron scale structure remains stable up to the softening point of polystyrene. The experimental set-up used is described and the results obtained are discussed
Science and Technology for A Sustainable Human Development
At the end of the NanoWorld Conference in San Francisco 25 April 2018 it
was decided to formalize the move of the NanoWorld Journal Headquarter from
Texas (USA) at United Scientific Group to Bergamo (Europe) at Fondazione
ELBA Nicolini, moving also to Paris the Nano World Conferences from March
4-6 2019, previously in San Francisco and Boston
Langmuir-Blodgett Protein Multilayer Nanofilms by XFEL
Serial femtosecond crystallography (SFX) at X-ray free electron lasers (XFELs) has created many new opportunities for protein crystallography, including
radiation damage mitigation and the study of dynamics at room temperature. This field is rapidly evolving, requiring new methods of macromolecule organization
into diffracting arrays, since current methods of sample preparation and delivery are often the bottleneck which limits productivity. We propose here the LangmuirBlodgett (LB) protein nanofilm technology as a novel approach for direct \u201con chip\u201d protein molecules organization into the 3D ordered diffracting arrays. The combination of this advanced technology with the XFEL for fixed target SFX
has the potential to become an important tool for the structure determination of proteins that are difficult to crystallize, such as membrane proteins of life science interest and with pharmaceutical industry impac
Langmuir-Blodgett Technology for Drugs Production and Delivery: Insights and Implications from an In Silico Study
Insulin dynameomics here reported as a database pertaining to a series of
Molecular Dynamics (MD) ran for Protein Data Bank (PDB) entries consisting
in bioinformatics and molecular dynamics simulation of Langmuir-Blodgett
and classical insulin with and without insulin receptor, appears to have profound
implications for drug design and endocrinology. The slight differences in
conformation and dynamics here reported may indeed explain why LB-insulin is
more stable when binds to its receptor (lower free energy) and this could be useful
when designing new drugs and pharmaceuticals
A Worldwide Strategy for Energy, Environment and Space Should Emerge in Paris as the Priority of Humanity: A Review NanoWorld Journal
Abstract Climate change, need for alternative energy and increasing asteroids in size and number represent key issues yielding growing challenges that must be tackled with more determination by the world community. The meeting set in Paris for December by the United Nations represents an unique opportunity to outline a common strategy for them as here outlined
NAPPA-Based Vaccines for a New Proteogenomics Approach for Public Health
Vaccinology was developed for the first time in 1796 when Jenner
empirically implemented a vaccine against smallpox using animal-to-human
cowpox inoculation. Since then, it has become a very complex science due to
the merging of disciplines ranging from structural and functional, cellular and
molecular biology and immunology to bioinformatics and nanobiotechnology,
as well as systems biology and synthetic biology and engineering. In the frame
of evidence-based medicine (EBM), evidence-based vaccinology emerged
as an important sub-field: vaccinology has nowadays become more and more
predictive and personalized. With the discovery that many patients with cancer
develop antibodies against p53 (the so-called oncoantibodies), it was evident
that oncoprotein are immunogenic and can be used for immunotherapeutics
purposes. In this manuscript, we report Nucleic Acid Programmable Protein
Arrays (NAPPA)-based Quartz Crystal Microbalance (QCM) measurement of
p53 immunogenicity and kinetics, in the perspective of developing an effective
p53 therapy. NAPPA-based QCM_D can be a useful platform for proving
the immunogenicity of oncoprotein-based vaccines. Recently, the field of
vaccinology has extended from vaccines for infectious diseases to vaccines not
only preventive but also therapeutic for chronic-degenerative diseases such as
cancer. Peptide-based immunotherapeutics has been proven to be quite effective
for cancer treatment and NAPPA-based QCM_D has the promise of providing
clinicians with quick, rapid and cheap measurement of oncoprotein kinetics and
bindings with immune cells. Moreover, it can be a precious tool for implementing
personalized and predictive vaccinology
Synchrotron powder diffraction study of radiation damage in Langmuir Blodgett nanotemplate crystallised protein
Polycrystalline samples of lysozyme were prepared with and without a Langmuir-Blodgett (LB) thin film template via both the hanging drop method and batch crystallisation. Powder diffraction methods are used to compare these samples and to measure their resistance to radiation damage at room temperature. The X-ray induced amorphisation of the samples was followed as a function of time and it was shown that diffraction does not entirely disappear even at very long exposure times. Two distinct kinetic timescales are evident suggesting that early and late stage processes are quite different. Radiation damage was also shown to be localized in the sample in the region where the beam impinges
Protein Crystallization by Anodic Porous Alumina (APA) Template: The Example of Hen Egg White Lysozyme (HEWL)
In this communication, we report anodic porous alumina (APA) template induced crystallization. The APA nanotemplate was prepared on the glass substrate for the hen egg white lysozyme (HEWL) crystal growth. The changes in the lysozyme crystals morphology, namely in the a/c axis ratio, were observed in the crystal grown by APA nanotemplate, but not in the crystal obtained with classical hanging drop vapor diffusion method, under the same experimental conditions. The comparison of the diffraction data of the two crystals as well as bioinformatics and data mining approaches and molecular dynamics simulations suggest a possible explanation of the nanotemplate crystallization phenomenon and shed light on the APA-induced nanocrystallography
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