Analysis of microtubule movement on isolated Xenopus egg cortices provides evidence that the cortical rotation involves dynein as well as Kinesin Related Proteins and is regulated by local microtubule polymerisation

AbstractIn amphibians, the cortical rotation, a translocation of the egg cortex relative to the cytoplasm, specifies the dorsoventral axis. The cortical rotation involves an array of subcortical microtubules whose alignment is mediated by Kinesin–related proteins (KRPs), and stops as M-phase promoting factor (MPF) activation propagates across the egg. To dissect the role of different motor proteins in the cortical rotation and to analyse their regulation, we have developed an open cell assay system involving reactivation of microtubule movement on isolated cortices. Microtubule movements were dependent on ATP and consisted mainly of wriggling and flailing without net displacement, consistent with a tethering of microtubules to the cortex. Reactivated movements were inhibited by anti-KRP and anti-dynein antibodies perfused together but not separately, the KRP antibody alone becoming fixed to the cortex. Neither antibody could inhibit movement in the presence of MPF, indicating that arrest of the cortical rotation is not due to MPF-dependent inhibition of motor molecules. In contrast, D2O treatment of live eggs to protect microtubules from progressive depolymerisation prolonged the cortical rotation. We conclude that the cortical rotation probably involves cytoplasmic dynein as well as cortical KRPs and terminates as a result of local MPF-dependent microtubule depolymerisation

Replacing home visits with telephone calls to support parents implementing a sleep management intervention : findings from a pilot study and implications for future research

Background - Resource constraints may inhibit the provision of appropriate interventions for children with neurodisabilities presenting with behavioural sleep problems. Telephone calls (TC), as opposed to home visits (HV), may be a more resource efficient means of supporting these families. Objective - To conduct a preliminary investigation exploring the feasibility and acceptability of replacing HV with TC to support parents implementing sleep management strategies and to gather evidence to inform the design and methods of a full trial. Methods - Parents referred to a sleep management intervention routinely delivered by a community paediatric team were alternately allocated to receive implementation support via HV (n = 7) or TC (n = 8). Activity logs recorded the frequency, duration and mode of support. Parents and practitioners were interviewed about their experiences of receiving/delivering the intervention. Results - Intervention drop-out was low, the frequency, number of contacts and intervention duration appeared comparable. Parents allocated TC received less contact time. Parents valued implementation support irrespective of delivery mode and practitioners reported that despite initial reservations, implementation support via TC appeared to work well. Conclusions - TC appears an acceptable and convenient mode of delivering sleep support, valued by both parents and practitioners. We recommend a full-scale trial to investigate effectiveness

Examining the relationships between attendance, online engagement and summative examinations performance

Background: Non-attendance correlates with poor performance, but manual recording of attendance is problematic. Online activity reports may be a more efficient method of identifying at-risk students. Summary of work: This research is part of a prospective study examining physical attendance, online activity reports (Moodle), continuous assessments and summative examination performance. Ethical approval was granted by RCSI Ethics Committee. Two modules within the first year of the undergraduate medical program were identified for inclusion. Results: Data from 2 RCSI modules are presented (NM and AS). A single cohort of 365 students undertook both modules, 30 of whom were repeating. Comparison of medians showed significant reductions in all parameters within the repeat student group. In NM, regression analysis showed continuous assessment had the largest effect size on summative examinations for both first-time and repeat student groups (R2 = 0.545; R2 = 0.289). Among repeat students, online access of lecture notes had a larger effect size than physical attendance at small group tutorials, while both these indices were less contributory (R2 \u3c 0.1) for first-time students. In AS, continuous assessment showed the largest effect size for first-time students (R2 = 0.585), while online access of lecture notes was most contributory among repeat students (R2 = 0.35). Conclusions: Effect sizes are most notable for continuous assessment, but online activity correlates with summative performance and is more predictive for outcomes among repeat students than physical attendance. These indices may be useful to screen at-risk students for individual intervention and support

Keeping children safe : allegations concerning the abuse or neglect of children in care

For most looked after children and young people, foster and residential care provides a safe environment. This study has focused on the minority of children who do not always receive safe care and who, in some instances, experience abuse or neglect at the hands of those responsible for ensuring their wellbeing. Despite long-standing concerns about historic abuse in children's homes and about the implications of allegations for foster carers and their families, very little is known about the extent of these allegations. We know even less about the proportion of allegations that are substantiated, the nature of the abuse and neglect experienced by some children in care settings and the characteristics of the adults and children involved. The aim of this study was to investigate these important questions. It provides new UK evidence on: * the number of allegations against foster carers and residential social workers and the proportion of these that are substantiated * the extent and nature of confirmed abuse and neglect in foster and residential care * the characteristics of the children and adults concerned

Investing in health R&D: where we are, what limits us, and how to make progress in Africa

Global research and development (R&D) pipelines for diseases that disproportionately affect African countries appear to be inadequate, with governments struggling to prioritise investment in R&D. This article provides insights into the sources of investment in health science research, available research capacity and level of research output in Africa. The African region comprises 15% of the world's population, yet only accounted for 1.1% of global investments in R&D in 2016. There were substantial disparities within the continent, with Egypt, Nigeria and South Africa contributing 65.7% of the total R&D spending. In most countries of the Organisation for Economic Co-operation and Development, the largest source of R&D funding is the private sector. R&D in Africa is mainly funded by the public sector, with significant proportions of financing in many countries coming from international funding. Challenges that limit private sector investment include unstable political environments, poor governance and corruption. Evidence suggests various research output and research capacity limitations in Africa when considering a global context. Metrics that reflect this include university rankings, number of researchers, number of publications, clinical trials networks and pharmaceutical manufacturing capacity. Within the continent there are substantial regional disparities. Incentivising investment is crucial to foster current and future research output and research capacity. This paper outlines some of the many commendable initiatives under way. Innovative and collaborative financing mechanisms can stimulate further investment. Given the vast inequalities across Africa in R&D, strategies need to reflect the different capacities of countries to address this disparity

The soil microbiome at the Gi-FACE experiment responds to a moisture gradient but not to CO2 enrichment

The soil bacterial community at the Giessen free-air CO2 enrichment (Gi-FACE) experiment was analysed by tag-sequencing of the 16S rRNA gene. No substantial effects of CO2 levels on bacterial community composition were detected. However, the soil moisture gradient at Gi-FACE had a significant effect on bacterial community composition. Different groups within the Acidobacteria and Verrucomicrobia phyla were affected differently by soil moisture content. These results suggest that modest increases in atmospheric CO2 may cause only minor changes in soil bacterial community composition and indicate that the functional responses of the soil community to CO2 enrichment previously reported at Gi-FACE are due to other factors other than changes in bacterial community composition. These results suggest that modest increases in atmospheric CO2 may cause only minor changes in soil bacterial community composition and indicate that the soil functional responses to CO2 enrichment previously reported at Gi-FACE are due to factors other than changes in bacterial community composition. The effects of the moisture gradient revealed new information about the relationships between poorly known Acidobacteria and Verrucomicrobia and soil moisture content. This study contrasts with the relatively small number of other temperate grassland FACE microbiome studies in the use of moderate CO2 enrichment and the resulting minor changes in the soil microbiome. Thus, it will facilitate the development of further climate change mitigation studies. In addition, the moisture gradient found at Gi-FACE contributes new to knowledge in soil microbial ecology, particularly regarding the abundance and moisture relationships of the soil Verrucomicrobia

Fabrication of Thin, Luminescent, Single-crystal Diamond Membranes

The formation of single-crystal diamond membranes is an important prerequisite for the fabrication of high-quality optical cavities in this material. Diamond membranes fabricated using lift-off processes involving the creation of a damaged layer through ion implantation often suffer from residual ion damage, which severely limits their usefulness for photonic structures. The current work demonstrates that strategic etch removal of the most highly defective material yields thin, single-crystal diamond membranes with strong photoluminescence and a Raman signature approaching that of single-crystal bulk diamond. These optically-active membranes can form the starting point for fabrication of high-quality optical resonators.Comment: To appear in AP

Household finances of Carer's Allowance recipients

This report presents findings from qualitative research into the experiences of people claiming or with an underlying entitlement to Carer's Allowance (CA). The findings are based on in-depth interviews with 44 people who claimed CA or had an underlying entitlement to it, in three areas of the UK in summer 2013. The aim of the research was to better understand the following research questions: • How is CA viewed in terms of the household budget, and does this vary by type of household? • How do recipients use CA and what would be the impact of non-receipt? • How does the use of CA vary depending on who is being cared for and where? • What led to the decision to claim CA? • Has receipt of CA had different impacts at different times of the caring career? • What impact has caring had on labour market participation