Neurotrophin and Trk expression by cells of the human lamina cribrosa following oxygen-glucose deprivation

BACKGROUND: Ischemia within the optic nerve head (ONH) may contribute to retinal ganglion cell (RGC) loss in primary open angle glaucoma (POAG). Ischemia has been reported to increase neurotrophin and high affinity Trk receptor expression by CNS neurons and glial cells. We have previously demonstrated neurotrophin and Trk expression within the lamina cribrosa (LC) region of the ONH. To determine if ischemia alters neurotrophin and Trk protein expression in cells from the human LC, cultured LC cells and ONH astrocytes were exposed to 48 hours of oxygen-glucose deprivation (OGD). Also cells were exposed to 48 hours of OGD followed by 24 hours of recovery in normal growth conditions. Cell number, neurotrophin and Trk receptor protein expression, neurotrophin secretion, and Trk receptor activation were examined. RESULTS: Cell number was estimated using an assay for cell metabolism following 24, 48 and 72 hours of OGD. A statistically significant decrease in LC and ONH astrocyte cell number did not occur until 72 hours of OGD, therefore cellular protein and conditioned media were collected at 48 hours OGD. Protein expression of NGF, BDNF and NT-3 by LC cells and ONH astrocytes increased following OGD, as did NGF secretion. Recovery from OGD increased BDNF protein expression in LC cells. In ONH astrocytes, recovery from OGD increased NGF protein expression, and decreased BDNF secretion. Trk A expression and activation in LC cells was increased following OGD while expression and activation of all other Trk receptors was decreased. A similar increase in Trk A expression and activation was observed in ONH astrocytes following recovery from OGD. CONCLUSIONS: In vitro conditions that mimic ischemia increase the expression and secretion of neurotrophins by cells from the ONH. Increased Trk A expression and activation in LC cells following OGD and in ONH astrocytes following recovery from OGD suggest autocrine/paracrine neurotrophin signaling could be a response to ONH ischemia in POAG. Also, the increase in NGF, BDNF and NT-3 protein expression and NGF secretion following OGD also suggest LC cells and ONH astrocytes may be a paracrine source of neurotrophins for RGCs

Response of Bose gases in time-dependent optical superlattices

The dynamic response of ultracold Bose gases in one-dimensional optical lattices and superlattices is investigated based on exact numerical time evolutions in the framework of the Bose-Hubbard model. The system is excited by a temporal amplitude modulation of the lattice potential, as it was done in recent experiments. For regular lattice potentials, the dynamic signatures of the superfluid to Mott-insulator transition are studied and the position and the fine-structure of the resonances is explained by a linear response analysis. Using direct simulations and the perturbative analysis it is shown that in the presence of a two-colour superlattice the excitation spectrum changes significantly when going from the homogeneous Mott-insulator the quasi Bose-glass phase. A characteristic and experimentally accessible signature for the quasi Bose-glass is the appearance of low-lying resonances and a suppression of the dominant resonance of the Mott-insulator phase.Comment: 20 pages, 9 figures; added references and corrected typo

Policy opportunities

Recommendations are given regarding National Science Foundation (NSF) astronomy programs and the NASA Space Astrophysics program. The role of ground based astronomy is reviewed. The role of National Optical Astronomy Observatories (NOAO) in ground-based night-time astronomical research is discussed. An enhanced Explored Program, costs and management of small and moderate space programs, the role of astrophysics within NASA's space exploration initiative, suborbital and airborne astronomical research, the problems of the Hubble Space Telescope, and astronomy education are discussed. Also covered are policy issues related to the role of science advisory committees, international cooperation and competition, archiving and distribution of astronomical data, and multi-wavelength observations of variable sources

Adding Soft-Skills to the Hard Target of Adequacy: The Case for Rearticulation Based on a Multifocal Analysis

The purpose of this study is to expand the definition of adequacy by adding soft skills as a measure of school productivity. The singular focus on academic standards inherent in education policy has prevented scholars from seeing the concept of adequacy through myriad perspectives and has contributed to a resegregation of schools. Education policy includes legal, historical, and political perspectives; research inquiries must accommodate these multiple foci. This study made use of multifocal analysis to investigate the development of the concept of adequacy in South Carolina. Conclusions suggest an expanded definition of adequacy has potential for addressing school financing policy, but also for making historical, political and legal contributions to educational and economic policies aimed at repurposing schools

Use of Combined Systemic Hypothermia and Local Heat Treatment to Enhance Temperature Differences Between Tumor and Normal Tissues

The feasibility of combining local heat treatment with wholebody hypothermia in an effort to improve therapeutic gain was assessed. Superficial, non perfused phantom tumors were fashioned in eight anesthetized mongrel dogs by transplantation of the spleen from the abdomen to a subcutaneous site on the hind limb. After pretreatment of the animal with the vasodilator hydralazine (0.5 mg/kg, IV) to enhance normal tissue perfusion, the spleen implant was heated with a 2450-MHz microwave diathermy apparatus, first with the animal\u27s core body temperature in the normal range (39°C) and then after the animal had been packed in ice to reduce core temperature to 30°C. Applied power density and temperatures in both the phantom tumor and underlying muscle tissue were recorded during brief interruptions of diathermy until steady-state temperatures had been achieved. Under normothermic conditions with time-averaged applied power of 0.038 W/ml to phantom tumor and 0.014 W/ml to underlying muscle, tumor temperature rose to 45.9 ± l.8°C, while muscle temperature remained at 40.5 ± 0.7°C. During whole-body hypothermia applied power could be increased to 0.114 W/ml in phantom tumor and to 0.025 W/ml in muscle. Muscle temperature rose only to 33.8 ± l.6°C, while that of the nonperfused phantom tumor rose to 53.6 ± 4.3°C with systemic hypothermia. These results are in agreement with predictions based on the bioheat transfer equation, i.e., heat extraction from well-perfused normal tissues is greatly augmented by cooling of the arterial blood, allowing greater power input to the tumor-bearing region, higher tumor temperatures, and enhanced therapeutic gain during local heat treatments of poorly perfused tumor nodules

Subsonic and Transonic Wind Tunnel Testing of Two Inflatable Aerodynamic Decelerators

Two inflatable aerodynamic decelerator designs were tested in the Transonic Dynamics Tunnel at the NASA Langley Research Center: a tension cone and an isotensoid. The tension cone consists of a flexible tension shell attached to a torus and the isotensoid employs a ram-air inflated envelope. Tests were conducted at Mach numbers from 0.3 to 1.08 and Reynolds numbers from 0.59 to 2.46 million. The main objective of these tests was to obtain static aerodynamic coefficients at subsonic and transonic speeds to supplement supersonic aerodynamic data for these same two designs. The axial force coefficients of both designs increased smoothly from subsonic through transonic Mach numbers. Dynamic data show significant oscillation of the tension cone and minimal oscillation of the isotensoid. The transonic and subsonic data will be used to assemble an inflatable decelerator aerodynamic database for use in computational analyses and system studies

Spitzer, Near-Infrared, and Submillimeter Imaging of the Relatively Sparse Young Cluster, Lynds 988e

We present {\it Spitzer} images of the relatively sparse, low luminosity young cluster L988e, as well as complementary near-infrared (NIR) and submillimeter images of the region. The cluster is asymmetric, with the western region of the cluster embedded within the molecular cloud, and the slightly less dense eastern region to the east of, and on the edge of, the molecular cloud. With these data, as well as with extant H$\alpha$ data of stars primarily found in the eastern region of the cluster, and a molecular $^{13}$CO gas emission map of the entire region, we investigate the distribution of forming young stars with respect to the cloud material, concentrating particularly on the differences and similarities between the exposed and embedded regions of the cluster. We also compare star formation in this region to that in denser, more luminous and more massive clusters already investigated in our comprehensive multi-wavelength study of young clusters within 1 kpc of the Sun.Comment: 21 pages, 6 tables, 13 figures. Full resolution figures at: http://astro.pas.rochester.edu/~tom/Preprints/L988e.pd