The Institute of Archaeology & Siegfried H. Horn Museum Newsletter Volume 32.4

Jordan Field School 2011, Øystein S. LaBianca \u27Umayri 2011, Douglas R. Clark Random Surveyhttps://digitalcommons.andrews.edu/iaham-news/1048/thumbnail.jp

Polar Bear Conservation in Canada: Defining the Policy Problems

Conservation of polar bears (Ursus maritimus) in Canada is based on the goals and principles of the 1973 International Agreement on the Conservation of Polar Bears and Their Habitat, and has long been considered an exemplar of science-based wildlife management. However, accelerating social and ecological changes in the Arctic raise questions about the polar bear management regime’s ability to adapt successfully to new challenges. We apply the analytic framework of the policy sciences to develop a comprehensive orientation to this evolving situation, and we suggest possible ways to define and advance shared goals of stakeholders and other participants. We conclude that the decision process in polar bear management does not sufficiently foster identification and securing of common interests among participants who express multiple, competing perspectives in an arena that has been increasingly fragmented and symbolically charged by issues such as the recent listing of polar bears under the U.S. Endangered Species Act. The fundamental challenge for polar bear conservation in Canada is to design a better decision process so that it can constructively reconcile the various perspectives, demands, and expectations of stakeholders.Au Canada, la conservation des ours polaires (Ursus maritimus) respecte les objectifs et les principes de l’Accord international sur la conservation des ours blancs et leur habitat de 1973, qui est considéré depuis longtemps comme un modèle de gestion de la faune fondée sur la science. Cependant, l’évolution de plus en plus rapide des changements d’ordre social et écologique dans l’Arctique a pour effet de soulever des questions sur l’aptitude du régime de gestion de l’ours polaire à bien s’adapter aux nouveaux défis. Nous utilisons le cadre de référence analytique de la science des politiques pour aboutir à une orientation exhaustive de cette situation en pleine évolution, et nous suggérons des manières possibles de définir et de formuler des objectifs partagés par les parties prenantes et d’autres participants. Nous concluons que le processus de décision en matière de gestion de l’ours polaire n’encourage pas suffisamment l’identification et l’engagement d’intérêts communs entre les participants qui expriment des perspectives multiples et concurrentes dans un domaine de plus en plus fragmenté et symboliquement caractérisé par des enjeux tels que la liste récente d’ours polaires en vertu de la loi américaine sur les espèces en voie de disparition (U.S. Endangered Species Act). Le défi fondamental en ce qui a trait à la conservation des ours polaires au Canada consiste à concevoir un meilleur processus de décision pouvant réconcilier, de manière constructive, les diverses perspectives, exigences et attentes des parties prenantes

Laser Guide Star for Large Segmented-Aperture Space Telescopes, Part I: Implications for Terrestrial Exoplanet Detection and Observatory Stability

Precision wavefront control on future segmented-aperture space telescopes presents significant challenges, particularly in the context of high-contrast exoplanet direct imaging. We present a new wavefront control architecture that translates the ground-based artificial guide star concept to space with a laser source aboard a second spacecraft, formation flying within the telescope field-of-view. We describe the motivating problem of mirror segment motion and develop wavefront sensing requirements as a function of guide star magnitude and segment motion power spectrum. Several sample cases with different values for transmitter power, pointing jitter, and wavelength are presented to illustrate the advantages and challenges of having a non-stellar-magnitude noise limited wavefront sensor for space telescopes. These notional designs allow increased control authority, potentially relaxing spacecraft stability requirements by two orders of magnitude, and increasing terrestrial exoplanet discovery space by allowing high-contrast observations of stars of arbitrary brightness.Comment: Submitted to A

Mutagenesis of Trichoderma reesei endoglucanase I: impact of expression host on activity and stability at elevated temperatures.

BackgroundTrichoderma reesei is a key cellulase source for economically saccharifying cellulosic biomass for the production of biofuels. Lignocellulose hydrolysis at temperatures above the optimum temperature of T. reesei cellulases (~50°C) could provide many significant advantages, including reduced viscosity at high-solids loadings, lower risk of microbial contamination during saccharification, greater compatibility with high-temperature biomass pretreatment, and faster rates of hydrolysis. These potential advantages motivate efforts to engineer T. reesei cellulases that can hydrolyze lignocellulose at temperatures ranging from 60-70°C.ResultsA B-factor guided approach for improving thermostability was used to engineer variants of endoglucanase I (Cel7B) from T. reesei (TrEGI) that are able to hydrolyze cellulosic substrates more rapidly than the recombinant wild-type TrEGI at temperatures ranging from 50-70°C. When expressed in T. reesei, TrEGI variant G230A/D113S/D115T (G230A/D113S/D115T Tr_TrEGI) had a higher apparent melting temperature (3°C increase in Tm) and improved half-life at 60°C (t1/2 = 161 hr) than the recombinant (T. reesei host) wild-type TrEGI (t1/2 = 74 hr at 60°C, Tr_TrEGI). Furthermore, G230A/D113S/D115T Tr_TrEGI showed 2-fold improved activity compared to Tr_TrEGI at 65°C on solid cellulosic substrates, and was as efficient in hydrolyzing cellulose at 60°C as Tr_TrEGI was at 50°C. The activities and stabilities of the recombinant TrEGI enzymes followed similar trends but differed significantly in magnitude depending on the expression host (Escherichia coli cell-free, Saccharomyces cerevisiae, Neurospora crassa, or T. reesei). Compared to N.crassa-expressed TrEGI, S. cerevisiae-expressed TrEGI showed inferior activity and stability, which was attributed to the lack of cyclization of the N-terminal glutamine in Sc_TrEGI and not to differences in glycosylation. N-terminal pyroglutamate formation in TrEGI expressed in S. cerevisiae was found to be essential in elevating its activity and stability to levels similar to the T. reesei or N. crassa-expressed enzyme, highlighting the importance of this ubiquitous modification in GH7 enzymes.ConclusionStructure-guided evolution of T. reesei EGI was used to engineer enzymes with increased thermal stability and activity on solid cellulosic substrates. Production of TrEGI enzymes in four hosts highlighted the impact of the expression host and the role of N-terminal pyroglutamate formation on the activity and stability of TrEGI enzymes