Analysis of Case-Parent Trios Using a Loglinear Model with Adjustment for Transmission Ratio Distortion

Transmission of the two parental alleles to offspring deviating from the Mendelian ratio is termed Transmission Ratio Distortion (TRD), occurs throughout gametic and embryonic development. TRD has been well-studied in animals, but remains largely unknown in humans. The Transmission Disequilibrium Test (TDT) was first proposed to test for association and linkage in case-trios (affected offspring and parents); adjusting for TRD using control-trios was recommended. However, the TDT does not provide risk parameter estimates for different genetic models. A loglinear model was later proposed to provide child and maternal relative risk (RR) estimates of disease, assuming Mendelian transmission. Results from our simulation study showed that case-trios RR estimates using this model are biased in the presence of TRD; power and Type 1 error are compromised. We propose an extended loglinear model adjusting for TRD. Under this extended model, RR estimates, power and Type 1 error are correctly restored. We applied this model to an intrauterine growth restriction dataset, and showed consistent results with a previous approach that adjusted for TRD using control-trios. Our findings suggested the need to adjust for TRD in avoiding spurious results. Documenting TRD in the population is therefore essential for the correct interpretation of genetic association studies

Advantages and Disadvantages of Traditional Abaca, Genetically Modified Abaca, and Cross Hybrid Abaca

Abaca (Musa Textilis Née), also known as Manila hemp, is a plant native to the Philippines, which expanded through different parts of Asia. It is a biodegradable and sustainable source of fiber known for its high quality. Due to viruses, poor government support, and lack of fundings, the abaca fiber industry is encountering obstacles in keeping up with the global demands. Over time, researchers developed new abaca types such as crossbred abaca and genetically modified abaca to solve these problems, but these have their own deficiencies as well. The purpose of the study was to identify, assess, and elaborate on the advantages and disadvantages of each of the abaca types. This was accomplished through the evaluation of literature and the collection of data in interviews. The study was a systematic review focusing on meta-synthesis, with information derived from previously published research or related literature and information from experts in the field. It was determined that traditional abaca is vastly preferred over genetically modified abaca and cross hybrid abaca, due to a number of reasons, including lack of research and economic viability. Traditional abaca was found to be more sustainable overall. It was recommended that traditional abaca should be promoted more to spread awareness, and that farmers should be educated regarding the proper process of caring for abaca plants. Stigma regarding cross hybrid and genetically modified abaca should be addressed as well

Ultracold Gas of Dipolar NaCs Ground State Molecules

We report on the creation of bosonic NaCs molecules in their absolute rovibrational ground state via stimulated Raman adiabatic passage. We create ultracold gases with up to 22,000 dipolar NaCs molecules at a temperature of 300(50) nK and a peak density of $1.0(4) \times 10^{12}$ cm$^{-3}$. We demonstrate comprehensive quantum state control by preparing the molecules in a specific electronic, vibrational, rotational, and hyperfine state. Employing the tunability and strength of the permanent electric dipole moment of NaCs, we induce dipole moments of up to 2.6 D. Dipolar systems of NaCs molecules are uniquely suited to explore strongly interacting phases in dipolar quantum matter.Comment: 6 pages, 5 figure

Efficient Pathway to NaCs Ground State Molecules

We present a study of two-photon pathways for the transfer of NaCs molecules to their rovibrational ground state. Starting from NaCs Feshbach molecules, we perform bound-bound excited state spectroscopy in the wavelength range from 900~nm to 940~nm, covering more than 30 vibrational states of the c \, ^3\Sigma^+, b \, ^3\Pi, and B \, ^1\Pi electronic states. Analyzing the rotational substructure, we identify the highly mixed c \, ^3\Sigma^+_1 \, |v=22 \rangle \sim b \, ^3\Pi_1 \, | v=54\rangle state as an efficient bridge for stimulated Raman adiabatic passage (STIRAP). We demonstrate transfer into the NaCs ground state with an efficiency of up to 88(4)\%. Highly efficient transfer is critical for the realization of many-body quantum phases of strongly dipolar NaCs molecules and high fidelity detection of single molecules, for example, in spin physics experiments in optical lattices and quantum information experiments in optical tweezer arrays.Comment: 17 pages, 8 figure

2D Visualization of the Psoriasis Transcriptome Fails to Support the Existence of Dual-Secreting IL-17A/IL-22 Th17 T Cells.

The present paradigm of psoriasis pathogenesis revolves around the IL-23/IL-17A axis. Dual-secreting Th17 T cells presumably are the predominant sources of the psoriasis phenotype-driving cytokines, IL-17A and IL-22. We thus conducted a meta-analysis of independently acquired RNA-seq psoriasis datasets to explore the relationship between the expression of IL17A and IL22. This analysis failed to support the existence of dual secreting IL-17A/IL-22 Th17 cells as a major source of these cytokines. However, variable relationships amongst the expression of psoriasis susceptibility genes and of IL17A, IL22, and IL23A were identified. Additionally, to shed light on gene expression relationships in psoriasis, we applied a machine learning nonlinear dimensionality reduction strategy (t-SNE) to display the entire psoriasis transcriptome as a 2-dimensonal image. This analysis revealed a variety of gene clusters, relevant to psoriasis pathophysiology but failed to support a relationship between IL17A and IL22. These results support existing theories on alternative sources of IL-17A and IL-22 in psoriasis such as a Th22 cells and non-T cell populations

A High Phase-Space Density Gas of NaCs Feshbach Molecules

We report on the creation of ultracold gases of bosonic Feshbach molecules of NaCs. The molecules are associated from overlapping gases of Na and Cs using a Feshbach resonance at 864.12(5) G. We characterize the Feshbach resonance using bound state spectroscopy, in conjunction with a coupled-channel calculation. By varying the temperature and atom numbers of the initial atomic mixtures, we demonstrate the association of NaCs gases over a wide dynamic range of molecule numbers and temperatures, reaching 70 nK for our coldest systems and a phase-space density (PSD) near 0.1. This is an important stepping-stone for the creation of degenerate gases of strongly dipolar NaCs molecules in their absolute ground state.Comment: 6 pages, 5 figures, supplemental materia

How Can I Help You?Eligibility Worker: Navigating Patients Through the Social Services Maze

Introduction: Vermont has programs to assist low income individuals in obtaining basic needs such as health insurance, food security, fuel assistance, housing and transportation. However, these services are often underutilized by eligible individuals. Major barriers to enrollment include lack of knowledge about available programs and their income cutoffs, cumbersome application processes, literacy barriers, and lack of transportation to application sites. In other states, efforts to reduce these barriers have included shortened application forms, removal of asset tests, mail-in applications, media outreach, and eligibility workers placed in outreach agencies. Many studies suggest that the presence of an eligibility worker at a community health center can help overcome some social service enrollment barriers.https://scholarworks.uvm.edu/comphp_gallery/1012/thumbnail.jp

Protocol of a systematic review and network meta-analysis for the prevention and treatment of perinatal depression

Introduction Perinatal depression is common and can often lead to adverse health outcomes for mother and child. Multiple pharmacological and non-pharmacological treatments have been evaluated against usual care or placebo controls in meta-analyses for preventing and treating perinatal depression compared. It is not yet established which of these candidate treatments might be the optimal approach for prevention or treatment. Methods and analysis A systematic review and Bayesian network meta-analyses will be conducted. Eight electronic databases shall be searched for randomised controlled trials that have evaluated the effectiveness of treatments for prevention and/or treatment of perinatal depression. Screening of articles shall be conducted by two reviewers independently. One network meta-analysis shall evaluate the effectiveness of interventions in preventing depression during the perinatal period. A second network meta-analysis shall compare the effectiveness of treatments for depression symptoms in women with perinatal depression. Bayesian 95% credible intervals shall be used to estimate the pooled mean effect size of each treatment, and surface under cumulative ranking area will be used to rank the treatments\u27 effectiveness. Ethics and dissemination We shall report our findings so that healthcare providers can make informed decisions on what might be the optimal approach for addressing perinatal depression to prevent cases and improve outcomes in those suffering from depression through knowledge exchange workshops, international conference presentations and journal article publications. PROSPERO registration number CRD42020200081