428 research outputs found
The Feynman effective classical potential in the Schr\"odinger formulation
New physical insight into the correspondence between path integral concepts
and the Schr\"odinger formulation is gained by the analysis of the effective
classical potential, that is defined within the Feynman path integral
formulation of statistical mechanics. This potential is related to the
quasi-static response of the equilibrium system to an external force. These
findings allow for a comprehensive formulation of dynamical approximations
based on this potential.Comment: 10 pages, 4 figure
Complexes of Pd(II) and Pt(II) with 9-Aminoacridine: Reactions with DNA and Study of Their Antiproliferative Activity
Four new metal complexes {M = Pd(II) or Pt(II)} containing the ligand 9-aminoacridine (9AA) were
prepared. The compounds were characterized by FT-IR and 1H, 13C, and 195Pt NMR spectroscopies. Crystal structure of the palladium complex of formulae [Pd(9AA)(μ-Cl)]2 · 2DMF was determined by X-ray diffraction. Two 9-acridine molecules in the imine form bind symmetrically to the metal ions in a bidentate fashion through the imine nitrogen atom and the C(1) atom of the aminoacridine closing a new five-membered ring. By reaction with phosphine or pyridine, the Cl bridges broke and compounds with general formulae [Pd(9AA)Cl(L)] (where L = PPh3 or py) were formed. A mononuclear complex of platinum of formulae [Pt(9AA)Cl(DMSO)] was
also obtained by direct reaction of 9-aminoacridine and the complex [PtCl2(DMSO2]. The capacity of the compounds to modify the secondary and tertiary structures of DNA was evaluated by means of circular dichroism and electrophoretic mobility. Both palladium and platinum compounds proved active in the modification of both the secondary and tertiary DNA structures. AFM images showed noticeable modifications of the morphology of the plasmid pBR322 DNA by the
compounds probably due to the intercalation of the complexes between base pairs of the DNA molecule. Finally, the palladium complex was tested for antiproliferative activity against three different human tumor cell lines. The results suggest that the palladium complex of formula [Pd(9AA)(μ-Cl)]2 has significant antiproliferative activity, although it is less active than cisplatin
Targeting of sterically stabilised pH-sensitive liposomes to human T-leukaemia cells
The main aim of this work was to develop novel targeted sterically stabilised pH-sensitive liposomes tailored to promote efficient intracellular delivery of therapeutic molecules into human T-leukaemia cells. Our results indicate that the targeting moiety (thiolated transferrin) was successfully coupled to the distal reactive maleimide terminus of poly(ethylene glycol)-phospholipid conjugates incorporated in the liposomal bilayer. Results from atomic force microscopy studies, performed to characterise vesicle surface topology, indicated that, to a certain extent, thiolated transferrin has the ability to associate in a non-specific manner with the lipid membrane of pegylated liposomes. This is an issue not commonly reported in the literature but which is crucial to demonstrate the targeting proof of principle. Nevertheless, fluorimetric studies together with confocal microscopy clearly demonstrate that liposomes bearing covalently coupled transferrin associate more extensively to human T-leukaemia cells in vitro than non-targeted liposomes. Cell mechanistic studies indicate that targeted liposomes bind specifically to transferrin receptors and are internalised via receptor-dependent endocytotic pathway. In addition, the biophysical features exhibited by the developed liposomes, namely their ability to promote pH-triggered cytoplasmic delivery of loaded material, make them promising delivery systems for in vivo targeting of therapeutic molecules to tumours.http://www.sciencedirect.com/science/article/B6T6C-4DVT9WH-1/1/5592c4a7248e7be29f239e55046f842
MoDeST: a compositional modeling formalism for hard and softly timed systems
This paper presents Modest (MOdeling and DEscription language for Stochastic Timed systems), a formalism that is aimed to support (i) the modular description of reactive system's behaviour while covering both (ii) functional and (iii) nonfunctional system aspects such as timing and quality-of-service constraints in a single specification. The language contains features such as simple and structured data types, structuring mechanisms like parallel composition and abstraction, means to control the granularity of assignments, exception handling, and non-deterministic and random branching and timing. Modest can be viewed as an overarching notation for a wide spectrum of models, ranging from labeled transition systems, to timed automata (and probabilistic variants thereof) as well as prominent stochastic processes such as (generalized semi-)Markov chains and decision processes. The paper describes the design rationales and details of the syntax and semantics
Surgical management of lower extremity lymphedema: A comprehensive review
Lymphedema refers to the accumulation of protein-rich fluid in the interstitial spaces. This can occur secondary to congenital malformation of the lymphatic channels or nodes or as a result of an insult that damages appropriately formed channels and nodes. Stagnant, protein-rich lymph initiates an inflammatory response that leads to adipocyte proliferation, fibrous tissue deposition, and increased susceptibility to infections. The end result is permanent disfigurement and dermal changes. Early and accurate diagnosis is essential, since lymphedema is a chronic and progressive problem. When lymphedema affects the lower extremity, it is important to manage it in a way that preserves function and mobility. Early diagnosis also allows for a proactive rather than reactive approach to treatment and utilization of novel physiologic procedures, such as lymphovenous anastomosis and vascularized lymph node transfer. Such interventions slow down disease progression and reduce morbidity by allowing the surgeon to salvage the remaining functional lymphatic channels.Whenphysiologicproceduresfailorwhenfacedwithadelayedpresentation, the addition of excisional procedures can provide a more comprehensive treatment of this debilitating disease. The aim of this article is to review the most current concepts in the surgical management of lower extremity lymphedema
Single-channel transmission in gold one-atom contacts and chains
We induce superconductivity by proximity effect in thin layers of gold and
study the number of conduction channels which contribute to the current in
one-atom contacts and atomic wires. The atomic contacts and wires are
fabricated with a Scanning Tunneling Microscope. The set of transmission
probabilities of the conduction channels is obtained from the analysis of the
characteristic curve which is highly non-linear due to multiple Andreev
reflections. In agreement with theoretical calculations we find that there is
only one channel which is almost completely open.Comment: 4 pages, 2 figures. To be published in Phys. Rev. B, Rapid
Communications (2003
Evolution of Robustness to Noise and Mutation in Gene Expression Dynamics
Phenotype of biological systems needs to be robust against mutation in order
to sustain themselves between generations. On the other hand, phenotype of an
individual also needs to be robust against fluctuations of both internal and
external origins that are encountered during growth and development. Is there a
relationship between these two types of robustness, one during a single
generation and the other during evolution? Could stochasticity in gene
expression have any relevance to the evolution of these robustness? Robustness
can be defined by the sharpness of the distribution of phenotype; the variance
of phenotype distribution due to genetic variation gives a measure of `genetic
robustness' while that of isogenic individuals gives a measure of
`developmental robustness'. Through simulations of a simple stochastic gene
expression network that undergoes mutation and selection, we show that in order
for the network to acquire both types of robustness, the phenotypic variance
induced by mutations must be smaller than that observed in an isogenic
population. As the latter originates from noise in gene expression, this
signifies that the genetic robustness evolves only when the noise strength in
gene expression is larger than some threshold. In such a case, the two
variances decrease throughout the evolutionary time course, indicating increase
in robustness. The results reveal how noise that cells encounter during growth
and development shapes networks' robustness to stochasticity in gene
expression, which in turn shapes networks' robustness to mutation. The
condition for evolution of robustness as well as relationship between genetic
and developmental robustness is derived through the variance of phenotypic
fluctuations, which are measurable experimentally.Comment: 25 page
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