La gestione della privacy nell\u27accesso ai dati clinici tramite LDAP

not availableNell\u27Istituto di Fisiologia Clinica del CNR all\u27attivit? di ricerca ? affiancata un\u27intensa attivit? assistenziale erogata tramite ricovero o prestazioni ambulatoriali. Negli ultimi anni il sistema informativo dell\u27Istituto ? cresciuto rapidamente fino a coprire in maniera capillare l\u27intera struttura distribuita sulle diverse sedi del territorio nazionale. Il sistema informativo ? quindi cresciuto nel tempo integrando i sottosistemi nati e sviluppati all\u27interno dei diversi reparti, ognuno con le proprie caratteristiche e peculiarit?. I problemi relativi alla sicurezza dei dati si sono conseguentemente moltiplicati e sono stati affrontati mediante l\u27utilizzo di diverse tecnologie e da vari punti di vista. La robustezza del sistema per? viene vanificata se le credenziali di accesso ai dati sono deboli o se una revoca dei diritti di accesso ai dati non si riflette sui meccanismi di autenticazione e autorizzazione implementati per lo specifico servizio. Le problematiche descritte sono state affrontate e risolte tramite l\u27utilizzo del protocollo LDAP (Lightweight Directory Access Protocol)

Comparative Protein Profile of Parathyroid Adenoma and Carcinoma: a Case Report

Parathyroid tumours are heterogeneous and parathyroid carcinoma (PC) is a rare endocrine malignancy, accounting for less than 1% of all cases of sporadic primary hyperparathyroidism. In clinical practice, PC diagnosis is challenging and in the majority of cases is made postoperatively at histology. Here, we report the case of a Caucasian man who presented an adenoma and a carcinoma localized in two different parathyroid glands. After surgical removal, tumour tissues were processed to obtain proteome profiles. Briefly, 40 µg aliquots of protein extracts from both adenoma and carcinoma samples were treated with cyanine dyes (Cy5, Cy2, and Cy3) and separated using Differential Gel Electrophoresis (DIGE). Comparative analysis resulted in 37 differentially expressed spots. For mass spectrometry identification, a preparative 2-DE was performed, gel was silver stained, protein spots were digested by trypsin and resulting peptides analyzed by LC-MS/MS. Among 37 differentially expressed proteins, 31 showed an increased expression in carcinoma compared to adenoma. The most significant proteins were Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1), Annexin A4 (ANXA4) and Chloride intracellular channel protein 1 (CLIC1) with fold change of 6, 3 and 2.3, respectively. Previous immunohystochemical studies have suggested UCHL1 together with parafibromin as markers of parathyroid carcinoma. Our proteomic results, obtained in the same patient for two different tumours, are a strong evidence in favour of UCHL1 and other proteins as candidate positive markers in differential diagnosis of parathyroid tumours. The use of proteomic can increase the knowledge of the molecular steps involved in PC pathogenesis

Open-source Teleconsulting System for International Cooperative Medical Decision Making in Congenital Heart Diseases

A project was developed by Heart Hospital in Massa, supported by Cuore un Mondo Association and Tuscany Region, for medical cooperation with Balkan Countries in diagnosis and care of congenital heart diseases. First step was to set up a telemedicine network aimed at multi-center cooperative medical decision making. Tele-echocardiography was initially implemented at pediatric clinical centres of Banja Luka, Rijeka and Tirana, using videoconference equipment for transmitting over Internet sequences of medical images. Actually videoconference systems are expensive, use proprietary technology and have limited functions. To overcome these limitations we designed a low-cost Open-Source-based device allowing both on-line streaming of video signals for real-time interaction and off-line transmission of diagnostic images (DICOM and not) in addition to videoconference and image storage/management functions. This device is also challenging for use also in remote Countries not able to acquire expensive medical technology

Parathyroid Carcinoma and Adenoma Co-existing in One Patient: Case Report and Comparative Proteomic Analysis.

editorial reviewedBACKGROUND/AIM: The lack of specific parathyroid carcinoma (PC) biomarkers in clinical practice points out the importance of analyzing the proteomic signature of this cancer. We performed a comparative proteomic analysis of PC and parathyroid adenoma (PA) co-existing in the same patient. PATIENTS AND METHODS: PC and PA were taken from a 63-year-old patient. Using two-dimensional differential gel electrophoresis (2D-DIGE) coupled to mass spectrometry we examined the differences between PC and PA proteins. For validation, additional PC and PA samples were obtained from 10 patients. Western blot analysis was used to validate the difference of expression observed with 2D-DIGE analysis. Bioinfomatic analysis was performed using QIAGEN's Ingenuity Pathways Analysis (IPA) to determine the predominant canonical pathways and interaction networks involved. RESULTS: Thirty-three differentially expressed proteins were identified in PC compared to PA. Among these, ubiquitin C-terminal hydrolase-L1 (UCH-L1) was highly overexpressed in PC. The result was confirmed by Western Blot analysis in additional PC samples. CONCLUSION: Our comparative proteomic analysis of co-existing neoplasms allowed detecting specific and peculiar differences between PC and PA overcoming population biological variability

Parathyroid Carcinoma and Adenoma Co-existing in One Patient: Case Report and Comparative Proteomic Analysis

Background/Aim: The lack of specific parathyroid carcinoma (PC) biomarkers in clinical practice points out the importance of analyzing the proteomic signature of this cancer. We performed a comparative proteomic analysis of PC and parathyroid adenoma (PA) co-existing in the same patient. Patients and Methods: PC and PA were taken from a 63-year-old patient. Using two-dimensional differential gel electrophoresis (2D-DIGE) coupled to mass spectrometry we examined the differences between PC and PA proteins. For validation, additional PC and PA samples were obtained from 10 patients. Western blot analysis was used to validate the difference of expression observed with 2D-DIGE analysis. Bioinfomatic analysis was performed using QIAGEN’s Ingenuity Pathways Analysis (IPA) to determine the predominant canonical pathways and interaction networks involved. Results: Thirty-three differentially expressed proteins were identified in PC compared to PA. Among these, ubiquitin C-terminal hydrolase-L1 (UCH-L1) was highly overexpressed in PC. The result was confirmed by Western Blot analysis in additional PC samples. Conclusion: Our comparative proteomic analysis of co-existing neoplasms allowed detecting specific and peculiar differences between PC and PA overcoming population biological variability