Age distribution, tobacco smoke habit and DNA ploidy status in oral potentially malignant disordes (OPMDs) and oral squamous cell carcinomas (OSCCs) patients.

<p>The bottom and the top of each box show the first and third quartile while the line inside the box represents the median (second quartile). The tips of the whiskers represent the minimum and the maximum data value. The number of patients for each category is indicated at the bottom of the corresponding box. The boxes corresponding to DNA diploid OPMDs/OSCCs are white while those corresponding to DNA aneuploid OPMDs/OSCCs have a striped pattern. Significant (MW test) P-values (P < 0.05) are indicated. The FDR q-value method was applied for multiple testing (n = 8) correction and the resulting q-values are indicated.</p

Patients’ characteristics and differences in age distribution among patient subgroups.

<p>Patients’ characteristics and differences in age distribution among patient subgroups.</p

Mean number of genomic aberrations (MNGA) per patient and age or smoking status in oral potentially malignant disordes (OPMDs) and oral squamous cell carcinomas (OSCCs) patients.

<p>The average number of aberrations per patient is represented as gray dots superimposed over boxes. These present a thick horizontal line indicating the median number per group, and delimitate the 25^th and 75^th percentile, while whiskers show the 95% confidence interval. A) average number of aberrations per patients’ age; B) average number of aberrations per patients’ smoking habit.</p

Patients’ tobacco consumption details (54 former and 76 current smokers).

<p>Patients’ tobacco consumption details (54 former and 76 current smokers).</p

Relationship between ploidy status, age, and smoke habit, in OPMDs^a and OSCCs^b patients.

<p>Relationship between ploidy status, age, and smoke habit, in OPMDs<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0184425#t004fn001" target="_blank">^a</a> and OSCCs<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0184425#t004fn002" target="_blank">^b</a> patients.</p

Additional file 3: Figure S3. of Cooperative antitumor activities of carnosic acid and Trastuzumab in ERBB2+ breast cancer cells

CA and Tz cooperative inhibition of ERBB2+ cell survival is transient. Blue lines represent cell survival of SKBR-3 and BT474 cells continuously cultured with CA + Tz (cultures #2). Red lines represent cell survival of SKBR-3 and BT474 cells cultured for up to 6d with CA + Tz and for further 8d with control medium (cultures #1). Cell survival is expressed as arbitrary units (A.U.) after exposure of cultures to Alamar Blue for 4 h. The arrow represents the time point at which the medium containing the two drugs was replaced with control medium in cultures #1. Mean values and standard deviation (indicated as vertical bars) from four independent replicates (n = 4) are shown. P < 0.001 (***) (TIFF 817 kb

Le Courrier

28 mai 18341834/05/28 (N148)

Additional file 6: Figure S6. of Cooperative antitumor activities of carnosic acid and Trastuzumab in ERBB2+ breast cancer cells

Representative DNA content histograms obtained by high resolution DNA flow cytometry from SKBR-3 and BT474 cells, top and bottom row respectively. X axes show DNA content measured as intensity of fluorescent light emitted by DNA bound DAPI at 435 nm; Y axes show number of nuclei (counts). Arrow heads indicate G0/G1 and G2/M peaks. Control treatment, Ctr; Carnosic acid treatment; CA; Trastuzumab treatment, Tz; Carnosic plus Trastuzumab treatment, CA + Tz. (TIFF 396 kb

Additional file 1: Table S1. of Cooperative antitumor activities of carnosic acid and Trastuzumab in ERBB2+ breast cancer cells

Antibodies used for immunoblot analysis. (DOCX 78 kb

Number of bioptic samples used to isolate the nuclei suspension and perform hr DNA-FCM analysis subdivided by oral mucosa subsite and histology.

<p>¹ Non-dysplastic oral potentially malignant disorder.</p><p>² Dysplastic oral potentially malignant disorder.</p><p>³ Oral squamous cell carcinoma, OSCC.</p><p>Number of bioptic samples used to isolate the nuclei suspension and perform hr DNA-FCM analysis subdivided by oral mucosa subsite and histology.</p